scholarly journals Quantitative Polymerase Chain Reaction for Transforming Growth Factor-b Applied to a Field Study of Fish Health in Chesapeake Bay Tributaries

2000 ◽  
Vol 108 (5) ◽  
pp. 447
Author(s):  
Craig A. Harms ◽  
Christopher A. Ottinger ◽  
Vicki S. Blazer ◽  
Christine L. Densmore ◽  
Laurence H. Pieper ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Field Study ◽  
Chesapeake Bay ◽  
Transforming Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Fish Health ◽  
Chain Reaction ◽  
Factor B ◽  
Polymerase Chain

A competitive quantitative polymerase chain reaction assay for bovine transforming growth factor-B1 mRNA

Life Sciences ◽  
1996 ◽  
Vol 59 (25-26) ◽  
pp. 2157-2165 ◽  
Author(s):  
Joseph J. Lanzillo ◽  
Erin K. Maloney ◽  
Alexander C. White ◽  
Joanne Stevens ◽  
Barry Fanburg
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Polymerase Chain Reaction Assay ◽  
Transforming Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Circulating microRNA Signature Associated to Interstitial Lung Abnormalities in Respiratory Asymptomatic Subjects

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1556
Author(s):  
Blanca Ortiz-Quintero ◽  
Ivette Buendía-Roldán ◽  
Eric Gustavo Ramírez-Salazar ◽  
Yalbi I Balderas-Martínez ◽  
Sandra Lizbeth Ramírez-Rodríguez ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Transforming Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Circulating Microrna ◽  
Chain Reaction ◽  
Lung Abnormalities ◽  
Polymerase Chain ◽  
Asymptomatic Subjects

Interstitial lung abnormalities (ILA) are observed in around 9% of older respiratory asymptomatic subjects, mainly smokers. Evidence suggests that ILA may precede the development of interstitial lung diseases and may evolve to progressive fibrosis. Identifying biomarkers of this subclinical status is relevant for early diagnosis and to predict outcome. We aimed to identify circulating microRNAs (miRNAs) associated to ILA in a cohort of respiratory asymptomatic subjects older than 60 years. We identified 81 subjects with ILA from our Lung-Aging Program in Mexico City (n = 826). We randomly selected 112 subjects without ILA (Ctrl) from the same cohort. Using polymerase chain reaction PCR-Array technology (24 ILA and 24 Ctrl, screening cohort) and reverse-transcriptase quantitative polymerase chain reaction (RT-qPCR) (57 ILA and 88 Ctr, independent validation cohort) we identified seven up-regulated miRNAs in serum of ILA compared to Ctrl (miR-193a-5p, p < 0.0001; miR-502-3p, p < 0.0001; miR-200c-3p, p = 0.003; miR-16-5p, p = 0.003; miR-21-5p, p = 0.002; miR-126-3p, p = 0.004 and miR-34a-5p, p < 0.005). Pathways regulated by these miRNAs include transforming growth factor beta (TGF-β), Wnt, mammalian target of rapamycin (mTOR), Insulin, mitogen-activated protein kinase (MAPK) signaling, and senescence. Receiver operator characteristic (ROC) curve analysis indicated that miR-193a-5p (area under the curve AUC: 0.75) and miR-502-3p (AUC 0.71) have acceptable diagnostic value. This is the first identification of circulating miRNAs associated to ILA in respiratory asymptomatic subjects, providing potential non-invasive biomarkers and molecular targets to better understand the pathogenic mechanisms associated to ILA.

Macrophage migration inhibitory factor suppresses transforming growth factor-β2 secretion in cultured rat testicular peritubular cells

2005 ◽  
Vol 17 (4) ◽  
pp. 435 ◽  
Author(s):  
Ruth Müller ◽  
Jörg Klug ◽  
Miriam Rodewald ◽  
Andreas Meinhardt
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inflammatory Cytokine ◽  
Transforming Growth Factor ◽  
Macrophage Migration ◽  
Direct Effects ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Peritubular Cells

Cytokines have direct effects on testicular cell functions and a number of cytokines are produced constitutively within the testis, even in the absence of immune-activation events. There is clear evidence that cytokines play a dual role as important regulatory factors in the normal function of the testis, as well as in testicular inflammation. The pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) is expressed locally in the testis and has direct effects on peritubular cells, which, in turn, produce anti-inflammatory mediators, including transforming growth factor (TGF)-β2. In the present study, we investigated the function of MIF by examining its effect on the secretion of TGF-β2 in peritubular cells. Expression of TGF-β2 mRNA was shown by reverse transcription–polymerase chain reaction in peritubular cells isolated from 19-day-old rat testis. The addition of recombinant MIF to cultured peritubular cells resulted in a dose-dependent decrease in TGF-β2 secretion up to 52% of control levels after 48 h, which was significant for all doses investigated (10–100 ng mL−1 MIF). Inhibition of TGF-β2 secretion was sustained for 72 h for the highest dose of MIF used (100 ng mL−1). No effect of MIF was observed on TGF-β2 mRNA expression levels, as shown by real-time polymerase chain reaction. These results suggest that the pro-inflammatory cytokine MIF can shift the cytokine balance from the immunosuppressive state towards an inflammatory reaction, potentially through the inhibition of TGF-β2 secretion by peritubular cells.

Gene Expression Profiling and Cancer-Related Pathways in Type I Endometrial Carcinoma

2010 ◽  
Vol 20 (5) ◽  
pp. 724-731 ◽  
Author(s):  
Fatma S.A. Saghir ◽  
Isa Mohamed Rose ◽  
Ahmad Zailani Hatta Mohd Dali ◽  
Zainab Shamsuddin ◽  
A Rahman A. Jamal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Normal Tissue ◽  
Transforming Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Rna Isolation ◽  
Gene Set Enrichment Analysis ◽  
Type I ◽  
Chain Reaction ◽  
Polymerase Chain

Introduction:Malignant transformation of type I endometrium involves alteration in gene expression with subsequent uncontrolled proliferation of altered cells.Objective:The main objective of the present study was to identify the cancer-related genes and gene pathways in the endometrium of healthy and cancer patients.Materials and Methods:Thirty endometrial tissues from healthy and type I EC patients were subjected to total RNA isolation. The RNA samples with good integrity number were hybridized to a new version of Affymetrix Human Genome GeneChip 1.0 ST array. We analyzed the results using the GeneSpring 9.0 GX and the Pathway Studio 6.1 software. For validation assay, quantitative real-time polymerase chain reaction was used to analyze 4 selected genes in normal and EC tissue.Results:Of the 28,869 genes profiled, we identified 621 differentially expressed genes (2-fold) in the normal tissue and the tumor. Among these genes, 146 were up-regulated and 476 were down-regulated in the tumor as compared with the normal tissue (P < 0.001). Up-regulated genes included the v-erb-a erythroblastic leukemia viral oncogene homolog 3 (ErbB3), ErbB4, E74-like factor 3 (ELF3), and chemokine ligand 17 (CXCL17). The down-regulated genes included signal transducer and activator transcription 5B (STAT5b), transforming growth factor β receptor III (TGFβ3), caveolin 1 (CAV1), and protein kinase C alpha (PKCA). The gene set enrichment analysis showed 10 significant gene sets with related genes (P < 0.05). The quantitative polymerase chain reaction of 4 selected genes using similar RNA confirmed the microarray results (P < 0.05).Conclusions:Identification of molecular pathways with their genes related to type I EC contribute to the understanding of pathophysiology of this cancer, probably leading to identifying potential biomarkers of the cancer.

scholarly journals EXPRESSION OF TRANSFORMING GROWTH FACTOR ALPHA BAMHI AND RSAI GENE VARIANTS ASSOCIATED WITH NON-SYNDROMIC CLEFT PALATE OF INDONESIAN SUBJECTS USING POLYMERASE CHAIN REACTION METHOD

2018 ◽  
Vol 11 (4) ◽  
pp. 351
Author(s):  
Ani Melani Maskoen ◽  
Saskia L Nasroen ◽  
Prima Nanda Fauziah ◽  
Eky Setiawan Soeria Soemantri ◽  
Basri A Gani
Keyword(s):  
Polymerase Chain Reaction ◽  
Risk Factor ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Gene Variants ◽  
Gene Variant ◽  
Chain Reaction ◽  
Factor Alpha ◽  
Polymerase Chain

 Objective: This study aimed to detect and analyze transforming growth factor alpha (TGFA) BamHI and RsaI gene variants which associated with the risk factor of non-syndromic cleft palate only (NS CPO) of Indonesian subject.Methods: This was case-control study using samples from 32 NS CPO subjects and 28 control subjects. DNA was extracted from venous blood, and the TGFA gene was amplified using polymerase chain reaction technique, then digestion product from TaqI and RsaI restriction enzyme was evaluated. Statistical analysis to determine significant differences of gene variant frequency among NS CPO subject and control was χ2. The odds ratio (OR) was used to determine a risk factor of NS CPO.Results: The study results showed that the TGFA BamHI gene variant was not identified in NS CPO among Indonesian but TGFA RsaI gene variant was identified. The frequency of TT/B1B1 homozygous mutant genotype was 80.0% in NS CPO subjects and 20.0% in control subjects (OR=3.857; 95% confidence interval=0.405–36.749).Conclusion: TGFA RsaI gene can be considered a risk factor of NS CPO compared TGFA BamH1 gene of Indonesian subjects.

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