scholarly journals Comparison between immunocytochemical and polymerase chain reaction techniques for detection of oestrogen receptor and transforming growth factor β in breast cancer

1996 ◽  
Vol 73 (10) ◽  
pp. 1255-1259 ◽  
Author(s):  
KD Amoils ◽  
L Seymour ◽  
WR Bezwoda
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Oestrogen Receptor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Β ◽  
Chain Reaction ◽  
Polymerase Chain

A competitive quantitative polymerase chain reaction assay for bovine transforming growth factor-B1 mRNA

Life Sciences ◽  
1996 ◽  
Vol 59 (25-26) ◽  
pp. 2157-2165 ◽  
Author(s):  
Joseph J. Lanzillo ◽  
Erin K. Maloney ◽  
Alexander C. White ◽  
Joanne Stevens ◽  
Barry Fanburg
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Polymerase Chain Reaction Assay ◽  
Transforming Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

Macrophage migration inhibitory factor suppresses transforming growth factor-β2 secretion in cultured rat testicular peritubular cells

2005 ◽  
Vol 17 (4) ◽  
pp. 435 ◽  
Author(s):  
Ruth Müller ◽  
Jörg Klug ◽  
Miriam Rodewald ◽  
Andreas Meinhardt
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inflammatory Cytokine ◽  
Transforming Growth Factor ◽  
Macrophage Migration ◽  
Direct Effects ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Peritubular Cells

Cytokines have direct effects on testicular cell functions and a number of cytokines are produced constitutively within the testis, even in the absence of immune-activation events. There is clear evidence that cytokines play a dual role as important regulatory factors in the normal function of the testis, as well as in testicular inflammation. The pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) is expressed locally in the testis and has direct effects on peritubular cells, which, in turn, produce anti-inflammatory mediators, including transforming growth factor (TGF)-β2. In the present study, we investigated the function of MIF by examining its effect on the secretion of TGF-β2 in peritubular cells. Expression of TGF-β2 mRNA was shown by reverse transcription–polymerase chain reaction in peritubular cells isolated from 19-day-old rat testis. The addition of recombinant MIF to cultured peritubular cells resulted in a dose-dependent decrease in TGF-β2 secretion up to 52% of control levels after 48 h, which was significant for all doses investigated (10–100 ng mL−1 MIF). Inhibition of TGF-β2 secretion was sustained for 72 h for the highest dose of MIF used (100 ng mL−1). No effect of MIF was observed on TGF-β2 mRNA expression levels, as shown by real-time polymerase chain reaction. These results suggest that the pro-inflammatory cytokine MIF can shift the cytokine balance from the immunosuppressive state towards an inflammatory reaction, potentially through the inhibition of TGF-β2 secretion by peritubular cells.

scholarly journals EXPRESSION OF TRANSFORMING GROWTH FACTOR ALPHA BAMHI AND RSAI GENE VARIANTS ASSOCIATED WITH NON-SYNDROMIC CLEFT PALATE OF INDONESIAN SUBJECTS USING POLYMERASE CHAIN REACTION METHOD

2018 ◽  
Vol 11 (4) ◽  
pp. 351
Author(s):  
Ani Melani Maskoen ◽  
Saskia L Nasroen ◽  
Prima Nanda Fauziah ◽  
Eky Setiawan Soeria Soemantri ◽  
Basri A Gani
Keyword(s):  
Polymerase Chain Reaction ◽  
Risk Factor ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Gene Variants ◽  
Gene Variant ◽  
Chain Reaction ◽  
Factor Alpha ◽  
Polymerase Chain

 Objective: This study aimed to detect and analyze transforming growth factor alpha (TGFA) BamHI and RsaI gene variants which associated with the risk factor of non-syndromic cleft palate only (NS CPO) of Indonesian subject.Methods: This was case-control study using samples from 32 NS CPO subjects and 28 control subjects. DNA was extracted from venous blood, and the TGFA gene was amplified using polymerase chain reaction technique, then digestion product from TaqI and RsaI restriction enzyme was evaluated. Statistical analysis to determine significant differences of gene variant frequency among NS CPO subject and control was χ2. The odds ratio (OR) was used to determine a risk factor of NS CPO.Results: The study results showed that the TGFA BamHI gene variant was not identified in NS CPO among Indonesian but TGFA RsaI gene variant was identified. The frequency of TT/B1B1 homozygous mutant genotype was 80.0% in NS CPO subjects and 20.0% in control subjects (OR=3.857; 95% confidence interval=0.405–36.749).Conclusion: TGFA RsaI gene can be considered a risk factor of NS CPO compared TGFA BamH1 gene of Indonesian subjects.

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