Rapid and inexpensive method of studying dedifferentiation in rabbit articular chondrocytes

Chondrocyte dedifferentiation is a cellular phenomenon in which chondrocyte loses its chondrogenic characteristics and ability to synthesize extracellular matrix. These conditions commonly reported in different cartilage degenerative diseases. The methods used to induce dedifferentiation in cell models of chondrocytes are often time consuming or require expensive reagents. Here in our protocol, we describe the utility of using 2-deoxy-D-glucose as a dedifferentiating agent for rabbit chondrocytes for studying dedifferentiation process.