Cloning and sequencing hybrid striped bass (Morone saxatilis x M. chrysops) transforming growth factor-β (TGF-β), and development of a reverse transcription quantitative competitive polymerase chain reaction (RT-qcPCR) assay to measure TGF-β mRNA of teleost fish

2000 ◽  
Vol 10 (1) ◽  
pp. 61-85 ◽  
Author(s):  
C.A Harms ◽  
S Kennedy-Stoskopf ◽  
W.A Horne ◽  
F.J Fuller ◽  
W.A.F Tompkins
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Striped Bass ◽  
Transforming Growth Factor ◽  
Morone Saxatilis ◽  
Transforming Growth Factor Β ◽  
Chain Reaction ◽  
Cloning And Sequencing ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

A competitive quantitative polymerase chain reaction assay for bovine transforming growth factor-B1 mRNA

Life Sciences ◽  
1996 ◽  
Vol 59 (25-26) ◽  
pp. 2157-2165 ◽  
Author(s):  
Joseph J. Lanzillo ◽  
Erin K. Maloney ◽  
Alexander C. White ◽  
Joanne Stevens ◽  
Barry Fanburg
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Polymerase Chain Reaction Assay ◽  
Transforming Growth Factor ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

Macrophage migration inhibitory factor suppresses transforming growth factor-β2 secretion in cultured rat testicular peritubular cells

2005 ◽  
Vol 17 (4) ◽  
pp. 435 ◽  
Author(s):  
Ruth Müller ◽  
Jörg Klug ◽  
Miriam Rodewald ◽  
Andreas Meinhardt
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inflammatory Cytokine ◽  
Transforming Growth Factor ◽  
Macrophage Migration ◽  
Direct Effects ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Peritubular Cells

Cytokines have direct effects on testicular cell functions and a number of cytokines are produced constitutively within the testis, even in the absence of immune-activation events. There is clear evidence that cytokines play a dual role as important regulatory factors in the normal function of the testis, as well as in testicular inflammation. The pro-inflammatory cytokine macrophage migration inhibitory factor (MIF) is expressed locally in the testis and has direct effects on peritubular cells, which, in turn, produce anti-inflammatory mediators, including transforming growth factor (TGF)-β2. In the present study, we investigated the function of MIF by examining its effect on the secretion of TGF-β2 in peritubular cells. Expression of TGF-β2 mRNA was shown by reverse transcription–polymerase chain reaction in peritubular cells isolated from 19-day-old rat testis. The addition of recombinant MIF to cultured peritubular cells resulted in a dose-dependent decrease in TGF-β2 secretion up to 52% of control levels after 48 h, which was significant for all doses investigated (10–100 ng mL−1 MIF). Inhibition of TGF-β2 secretion was sustained for 72 h for the highest dose of MIF used (100 ng mL−1). No effect of MIF was observed on TGF-β2 mRNA expression levels, as shown by real-time polymerase chain reaction. These results suggest that the pro-inflammatory cytokine MIF can shift the cytokine balance from the immunosuppressive state towards an inflammatory reaction, potentially through the inhibition of TGF-β2 secretion by peritubular cells.

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