scholarly journals The isolation of (6S, 9S)-cyclo (prolylvalyl) from marine actinomycete, by use of high speed contercurrent chromatography

2002 ◽  
Vol 67 (1) ◽  
pp. 27-30
Author(s):  
Andrija Smelcerovic ◽  
Mirko Schiebel ◽  
Sinisa Djordjevic
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
High Speed ◽  
Countercurrent Chromatography ◽  
Marine Research ◽  
Gel Fraction ◽  
Marine Actinomycete

The marine actinomycete B 1758 came from the actinomycete collection of the Alfred Wegener Institute for Polar and Marine Research in Bremerhafen Germany. 1.079 g of raw extract was obtained by fermentation. Seven fractions were separated by column chromatography on silica gel. Fraction 4 was separated by high speed countercurrent chromatography. Fraction 4.6 yielded 5.2 mg of (6S, 9S)-cyclo(prolylvalyl).

scholarly journals Systematic Separation and Purification of Alkaloids from Euchresta tubulosa Dunn. by Various Chromatographic Methods

Processes ◽  
2019 ◽  
Vol 7 (12) ◽  
pp. 924
Author(s):  
Wei-Xin Li ◽  
Huan Wang ◽  
Ai-Wen Dong
Keyword(s):  
Natural Products ◽  
Silica Gel ◽  
Column Chromatography ◽  
High Speed ◽  
High Performance ◽  
Ionization Mass ◽  
Separation And Purification ◽  
Two Phase ◽  
Rapid Separation ◽  
Chemical Structures

High-speed countercurrent chromatography (HSCCC) and silica gel column chromatography were used to separate and purify alkaloids from Chinese herbal medicine Euchresta tubulosa Dunn. The purpose of this study is to provide a system mode for rapid separation of alkaloids from natural products. In the experiment, the eluent of silica gel column chromatography was screened by thin layer chromatography (TLC) to obtain four components with different polarity. Then, the two-phase solvent systems of different components were selected and purified by HSCCC. Four alkaloids with relatively high content were obtained by this mode successfully, including matrine (28 mg), oxymatrine (32 mg), N-formyl cytisine (24 mg), and cytisine (58 mg). The purity was higher than 91% by high performance liquid chromatography–ultraviolet (HPLC-UV) and their chemical structures were identified by nuclear magnetic resonance (NMR) and electron ionization mass spectrometry (EI-MS). The results showed that the combination of HSCCC and silica gel column chromatography could make alkaloids from natural products separate systematically.

Purification of coenzyme Q10 from fermentation extract: High-speed counter-current chromatography versus silica gel column chromatography

2006 ◽  
Vol 1127 (1-2) ◽  
pp. 92-96 ◽  
Author(s):  
Xue-Li Cao ◽  
Ya-Tao Xu ◽  
Guang-Ming Zhang ◽  
Sheng-Meng Xie ◽  
Ying-Mao Dong ◽  
...  
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Coenzyme Q10 ◽  
High Speed ◽  
Counter Current

scholarly journals (6R,9S)-6″-(4″-Hydroxybenzoyl)-Roseoside, a New Megastigmane Derivative from Ouratea polyantha and its Effect on Hepatic Glucose-6-phosphatase

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Jairo Bermúdez ◽  
María Rodríguez ◽  
Masahisa Hasegawa ◽  
Freddy González-Mujica ◽  
Sandra Duque ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Column Chromatography ◽  
High Speed ◽  
Inhibitory Effect ◽  
Countercurrent Chromatography ◽  
Analytical Column ◽  
H Nmr ◽  
Hepatic Glucose ◽  
Counter Current ◽  
C Nmr

A new megastigmane derivative, (6 R,9 S)–6′-(4″-hydroxybenzoyl)-roseoside (1) and two known compounds, the biflavoneagathisflavone (2) and 4-hydroxy-benzoic acid (3) were isolated and purified from leaves and stems of Ouratea polyantha Engl. Agathisflavone was isolated in a single high-speed counter-current chromatography run, while the megastigmane was purified in two steps, by using a combination of high-speed countercurrent chromatography and analytical column chromatography. All structures were elucidated on the basis of spectral evidence and comparison with literature data. Compound 1 was characterized by [α]D20, UV-Vis, IR, MS, 1H NMR, 13C NMR, HMQC, HMBC, COSY and NOESY. Compounds 1 and 2 showed an inhibitory effect of 63.6 and 13.7% on the G-6-Pase intact microsomes, respectively.

scholarly journals Comparison of Cleanup Techniques for Simple Method for Analysis of Selected Organophosphorus Pesticide Residues in Molluscs

1996 ◽  
Vol 79 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Félix Hernández ◽  
Roque Serrano ◽  
Joaquim Beltran ◽  
Francisco J López
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Pesticide Residues ◽  
High Speed ◽  
Mytilus Galloprovincialis ◽  
Organophosphorus Pesticides ◽  
Organophosphorus Pesticide ◽  
Gas Chromatograph ◽  
Simple Method

Abstract A simple method for determination of 5 organo phosphorus pesticides (dimethoate, chlorfenvin phos, chlorpyrifos, methidathion, and phosmet) in molluscs (Mytilus galloprovincialis and Venus gallina) was developed, with special attention to cleanup. Organophosphorus pesticides were extracted with acetonitrile-acetone (90 + 10, v/v) in a high-speed blender. Two cleanup procedures were used to defat extracts prior to injection into a gas chromatograph: liquid-liquid partition with acetoni trile-hexane and adsorption column chromatography with silica gel. The latter was more efficient for elimination of fat and fractionation of pesticides with different polarities. Limits of detection of the overall procedure including extraction and cleanup ranged from 0.2 to 1 ng/g. Quantitative recoveries for pesticide concentrations ranging from 1 to 10 000 ng/g were obtained.

Isolation of glucosinolates from broccoli seeds by use of high-speed countercurrent chromatography

2008 ◽  
Vol 20 (3) ◽  
pp. 489-496 ◽  
Author(s):  
Q. Du ◽  
L. Zhang ◽  
H. Cui ◽  
K. Wang ◽  
L. Xiu ◽  
...  
Keyword(s):  
High Speed ◽  
Countercurrent Chromatography
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