Purification of coenzyme Q10 from fermentation extract: High-speed counter-current chromatography versus silica gel column chromatography

2006 ◽  
Vol 1127 (1-2) ◽  
pp. 92-96 ◽  
Author(s):  
Xue-Li Cao ◽  
Ya-Tao Xu ◽  
Guang-Ming Zhang ◽  
Sheng-Meng Xie ◽  
Ying-Mao Dong ◽  
...  
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Coenzyme Q10 ◽  
High Speed ◽  
Counter Current

scholarly journals The isolation of (6S, 9S)-cyclo (prolylvalyl) from marine actinomycete, by use of high speed contercurrent chromatography

2002 ◽  
Vol 67 (1) ◽  
pp. 27-30
Author(s):  
Andrija Smelcerovic ◽  
Mirko Schiebel ◽  
Sinisa Djordjevic
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
High Speed ◽  
Countercurrent Chromatography ◽  
Marine Research ◽  
Gel Fraction ◽  
Marine Actinomycete

The marine actinomycete B 1758 came from the actinomycete collection of the Alfred Wegener Institute for Polar and Marine Research in Bremerhafen Germany. 1.079 g of raw extract was obtained by fermentation. Seven fractions were separated by column chromatography on silica gel. Fraction 4 was separated by high speed countercurrent chromatography. Fraction 4.6 yielded 5.2 mg of (6S, 9S)-cyclo(prolylvalyl).

Isolation of two new prenylated flavonoids from Sinopodophyllum emodi fruit by silica gel column and high-speed counter-current chromatography

2014 ◽  
Vol 969 ◽  
pp. 190-198 ◽  
Author(s):  
Yanjun Sun ◽  
Yinshi Sun ◽  
Hui Chen ◽  
Zhiyou Hao ◽  
Junmin Wang ◽  
...  
Keyword(s):  
Silica Gel ◽  
High Speed ◽  
Prenylated Flavonoids ◽  
Counter Current

scholarly journals Systematic Separation and Purification of Alkaloids from Euchresta tubulosa Dunn. by Various Chromatographic Methods

Processes ◽  
2019 ◽  
Vol 7 (12) ◽  
pp. 924
Author(s):  
Wei-Xin Li ◽  
Huan Wang ◽  
Ai-Wen Dong
Keyword(s):  
Natural Products ◽  
Silica Gel ◽  
Column Chromatography ◽  
High Speed ◽  
High Performance ◽  
Ionization Mass ◽  
Separation And Purification ◽  
Two Phase ◽  
Rapid Separation ◽  
Chemical Structures

High-speed countercurrent chromatography (HSCCC) and silica gel column chromatography were used to separate and purify alkaloids from Chinese herbal medicine Euchresta tubulosa Dunn. The purpose of this study is to provide a system mode for rapid separation of alkaloids from natural products. In the experiment, the eluent of silica gel column chromatography was screened by thin layer chromatography (TLC) to obtain four components with different polarity. Then, the two-phase solvent systems of different components were selected and purified by HSCCC. Four alkaloids with relatively high content were obtained by this mode successfully, including matrine (28 mg), oxymatrine (32 mg), N-formyl cytisine (24 mg), and cytisine (58 mg). The purity was higher than 91% by high performance liquid chromatography–ultraviolet (HPLC-UV) and their chemical structures were identified by nuclear magnetic resonance (NMR) and electron ionization mass spectrometry (EI-MS). The results showed that the combination of HSCCC and silica gel column chromatography could make alkaloids from natural products separate systematically.

Separation of two constituents from purple sweet potato by combination of silica gel column and high-speed counter-current chromatography

2012 ◽  
Vol 881-882 ◽  
pp. 49-54 ◽  
Author(s):  
Kai He ◽  
Xiaoli Ye ◽  
Xuegang Li ◽  
Hongying Chen ◽  
Lujiang Yuan ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Silica Gel ◽  
High Speed ◽  
Purple Sweet Potato ◽  
Counter Current

scholarly journals Isolation and purification of lignans from Schisandra chinensis by combination of silica gel column and high-speed counter-current chromatography

2013 ◽  
Vol 19 (3) ◽  
pp. 435-440
Author(s):  
Yu Sun ◽  
Shuangshuang Xu ◽  
Yanling Geng ◽  
Xiao Wang ◽  
Tianyou Zhang
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Silica Gel ◽  
High Speed ◽  
Solvent System ◽  
Schisandra Chinensis ◽  
Two Phase ◽  
Chemical Structures ◽  
Gomisin A ◽  
Counter Current

Silica gel column combined with high-speed counter-current chromatography separation was successfully applied to the separation of schizandrin (I), angeloylgomisin H (II), gomisin A (III), schisantherin C (IV), deoxyschizandrin (V), ?-schisandrin (VI) and schisandrin C (VII) from the fruits of Schisandra chinensis (Turcz.) Baillon. The petroleum ether extracts of the fruits of S. chinensis were pre-separated first on a silica gel column and divided into two fractions as sample 1 and sample 2. 260 mg of sample 1 was separated by HSCCC using petroleum ether-ethyl acetate-methanol-water (10:8:10:8, v/v) as the two-phase solvent system and 18.2 mg of schizandrin, 15.7 mg of angeloylgomisin H, 16.5 mg of gomisin A and 16.7 mg of schisantherin C were obtained. 230 mg of sample 2 was separated using petroleum ether-ethyl acetate-methanol-water (10:0.5:10:1, v/v) as the two-phase solvent system and 19.7 mg of deoxyschizandrin, 23.4 mg of ?-schisandrin and 18.2 mg of schisandrin C were obtained. The purities of the separated compounds were all over 94% as determined by HPLC. The chemical structures of these compounds were confirmed by ESI-MS and 1H NMR.

scholarly journals Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

Química Nova ◽  
2013 ◽  
Vol 36 (6) ◽  
pp. 836-839
Author(s):  
Xikai Shu ◽  
Mei Wang ◽  
Daicheng Liu ◽  
Daijie Wang ◽  
Xiaojing Lin ◽  
...  
Keyword(s):  
Column Chromatography ◽  
High Speed ◽  
Preparative Separation ◽  
Counter Current

High-Speed Counter-Current Chromatography Combined with Column Chromatography for Isolation of Methyllycaconitine from Delphinium pseudocyanthum

2007 ◽  
Vol 66 (11-12) ◽  
pp. 949-951 ◽  
Author(s):  
DongYu Gu ◽  
Yi Yang ◽  
Jie Zhong ◽  
Haji Akber Aisa ◽  
TianYou Zhang
Keyword(s):  
Column Chromatography ◽  
High Speed ◽  
Counter Current

scholarly journals (6R,9S)-6″-(4″-Hydroxybenzoyl)-Roseoside, a New Megastigmane Derivative from Ouratea polyantha and its Effect on Hepatic Glucose-6-phosphatase

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Jairo Bermúdez ◽  
María Rodríguez ◽  
Masahisa Hasegawa ◽  
Freddy González-Mujica ◽  
Sandra Duque ◽  
...  
Keyword(s):  
Benzoic Acid ◽  
Column Chromatography ◽  
High Speed ◽  
Inhibitory Effect ◽  
Countercurrent Chromatography ◽  
Analytical Column ◽  
H Nmr ◽  
Hepatic Glucose ◽  
Counter Current ◽  
C Nmr

A new megastigmane derivative, (6 R,9 S)–6′-(4″-hydroxybenzoyl)-roseoside (1) and two known compounds, the biflavoneagathisflavone (2) and 4-hydroxy-benzoic acid (3) were isolated and purified from leaves and stems of Ouratea polyantha Engl. Agathisflavone was isolated in a single high-speed counter-current chromatography run, while the megastigmane was purified in two steps, by using a combination of high-speed countercurrent chromatography and analytical column chromatography. All structures were elucidated on the basis of spectral evidence and comparison with literature data. Compound 1 was characterized by [α]D20, UV-Vis, IR, MS, 1H NMR, 13C NMR, HMQC, HMBC, COSY and NOESY. Compounds 1 and 2 showed an inhibitory effect of 63.6 and 13.7% on the G-6-Pase intact microsomes, respectively.

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