A practicable strategy for enrichment and separation of four minor flavonoids including two isomers from barley seedlings by macroporous resin column chromatography, medium‐pressure LC, and high‐speed countercurrent chromatography

2019 ◽  
Vol 42 (9) ◽  
pp. 1717-1724 ◽  
Author(s):  
Nana Wang ◽  
Tao Chen ◽  
Xue Yang ◽  
Cheng Shen ◽  
Hongmei Li ◽  
...  
Keyword(s):  
Column Chromatography ◽  
High Speed ◽  
Countercurrent Chromatography ◽  
Macroporous Resin ◽  
Resin Column ◽  
Medium Pressure

scholarly journals The isolation of (6S, 9S)-cyclo (prolylvalyl) from marine actinomycete, by use of high speed contercurrent chromatography

2002 ◽  
Vol 67 (1) ◽  
pp. 27-30
Author(s):  
Andrija Smelcerovic ◽  
Mirko Schiebel ◽  
Sinisa Djordjevic
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
High Speed ◽  
Countercurrent Chromatography ◽  
Marine Research ◽  
Gel Fraction ◽  
Marine Actinomycete

The marine actinomycete B 1758 came from the actinomycete collection of the Alfred Wegener Institute for Polar and Marine Research in Bremerhafen Germany. 1.079 g of raw extract was obtained by fermentation. Seven fractions were separated by column chromatography on silica gel. Fraction 4 was separated by high speed countercurrent chromatography. Fraction 4.6 yielded 5.2 mg of (6S, 9S)-cyclo(prolylvalyl).

scholarly journals Preparative Purification of Total Flavonoids from Sophora tonkinensis Gagnep. by Macroporous Resin Column Chromatography and Comparative Analysis of Flavonoid Profiles by HPLC-PAD

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3200 ◽  
Author(s):  
Mengyang Hou ◽  
Wenzhong Hu ◽  
Zhilong Xiu ◽  
Aili Jiang ◽  
Lei Men ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Column Chromatography ◽  
High Performance ◽  
Total Flavonoids ◽  
Macroporous Resin ◽  
Technological Parameters ◽  
Resin Column ◽  
Photodiode Array Detection ◽  
Before And After ◽  
Sophora Tonkinensis

For the full development and utilization of Sophora tonkinensis Gagnep., this study was primarily intended to established a simple and efficient approach for the preparative purification of total flavonoids from S. tonkinensis by macroporous resin column chromatography (MRCC). The adsorption and desorption characteristics of the total flavonoids on ten macroporous resins were first studied, and AB-8 resin was chosen as the most suitable, and the adsorption data were best fitted to the pseudo-second-order kinetics model and Langmuir isotherm model. Furthermore, the technological parameters for the purification of the total flavonoids were optimized using column chromatography. After a sample one-step purification procedure, the content of the total flavonoids increased by about 4.76-fold from 12.14% to 57.82%, with a recovery yield of 84.93%. In addition, the comparative analysis of the flavonoid extracts before and after purification was performed by high-performance liquid chromatography coupled with photodiode-array detection (HPLC-PAD). The results showed that the contents of six major flavonoids in the purified product were all higher than before the purification. Therefore, the AB-8 MRCC established in this work was a promising method for the industrial-scale purification of the total flavonoids from S. tonkinensis.

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