Online hyphenation of extraction, Sephadex LH-20 column chromatography, and high-speed countercurrent chromatography: A highly efficient strategy for the preparative separation of andrographolide from Andrographis paniculata in a single step

2017 ◽  
Vol 40 (24) ◽  
pp. 4865-4871 ◽  
Author(s):  
Ying-Qi Zhang ◽  
Shan-Shan Wang ◽  
Chao Han ◽  
Jin-Fang Xu ◽  
Jian-Guang Luo ◽  
...  
Keyword(s):  
Column Chromatography ◽  
High Speed ◽  
Single Step ◽  
Andrographis Paniculata ◽  
Countercurrent Chromatography ◽  
Preparative Separation ◽  
Efficient Strategy ◽  
Highly Efficient ◽  
Online Hyphenation

scholarly journals The isolation of (6S, 9S)-cyclo (prolylvalyl) from marine actinomycete, by use of high speed contercurrent chromatography

2002 ◽  
Vol 67 (1) ◽  
pp. 27-30
Author(s):  
Andrija Smelcerovic ◽  
Mirko Schiebel ◽  
Sinisa Djordjevic
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
High Speed ◽  
Countercurrent Chromatography ◽  
Marine Research ◽  
Gel Fraction ◽  
Marine Actinomycete

The marine actinomycete B 1758 came from the actinomycete collection of the Alfred Wegener Institute for Polar and Marine Research in Bremerhafen Germany. 1.079 g of raw extract was obtained by fermentation. Seven fractions were separated by column chromatography on silica gel. Fraction 4 was separated by high speed countercurrent chromatography. Fraction 4.6 yielded 5.2 mg of (6S, 9S)-cyclo(prolylvalyl).

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