scholarly journals Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for Usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation

2015 ◽  
Vol 69 (6) ◽  
pp. 703-712 ◽  
Author(s):  
Ana Zugic ◽  
Milica Lukic ◽  
Marija Tasic-Kostov ◽  
Vanja Tadic ◽  
Ivana Arsic ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Clinical Relevance ◽  
Comparative Evaluation ◽  
Skin Infections ◽  
Topical Formulation ◽  
Preliminary Screening ◽  
Antimicrobial Potential ◽  
Physicochemical Stability ◽  
Skin Conditions ◽  
Alkyl Polyglucoside

Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG) - based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best antimicrobial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections.

scholarly journals Antimicrobial Activity of Cultivable Endophytic Fungi Associated with Hancornia Speciosa Gomes Bark

2017 ◽  
Vol 11 (1) ◽  
pp. 179-188 ◽  
Author(s):  
Mardonny Bruno de Oliveira Chagas ◽  
Irailton Prazeres dos Santos ◽  
Luis Claudio Nascimento da Silva ◽  
Maria Tereza dos Santos Correia ◽  
Janete Magali de Araújo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Endophytic Fungi ◽  
Stem Bark ◽  
Fermentation Medium ◽  
Preliminary Screening ◽  
Antimicrobial Potential ◽  
Methanolic Extracts ◽  
Rice Medium ◽  
Semi Solid ◽  
Hancornia Speciosa

Introduction:In this study, we evaluated the antimicrobial potential of cultivable endophytic fungi associated withHancornia speciosaGomes stem bark.Methods and Materials:Plant samples were collected in rainy (July 2010) and dry (January 2011) seasons. In total, 116 endophytic fungi strains were isolated from 90 fragments (64.4% frequency of colonization). Higher fungi frequency was observed in the rainy season (84.4%). The strains were grouped into 14 species; the most frequent werePhoma cava(13.8%),Colletotrichum gloeosporioides(12.1%), andLasiodiplodia theobromae(11.2%). Fungal diversity was similar in both the seasons. Among the 116 strains, 39 (33.6%) showed antimicrobial activity in preliminary screening. The ten most active isolates were subjected to semi-solid fermentation using rice or corn as substrates. Methanolic extracts were obtained from each fermentation medium and the minimum inhibitory (MIC) and minimum microbicide concentrations (MMC) were determined.Results:The best antimicrobial results (MIC < 100 µg/mL) were observed for fungi strains grown in rice medium:Aspergillus nigerFHS061 againstProteus mirabilis(MIC = 19 µg/mL) andStaphylococcus aureus(MIC = 39 µg/mL). These strains also showed good results when cultivated in corn medium againstP. mirabilis(MIC = 78 µg/mL).Conclusion:Thus, the stem bark ofH. speciosaharbors diverse endophytic fungi with antimicrobial potential.

scholarly journals Comparative evaluation of antimicrobial activity of three cements: new endodontic cement (NEC), mineral trioxide aggregate (MTA) and Portland

2009 ◽  
Vol 51 (3) ◽  
pp. 437-442 ◽  
Author(s):  
Mohammad Hasan Zarrabi ◽  
Maryam Javidi ◽  
Mahboube Naderinasab ◽  
Maryam Gharechahi
Keyword(s):  
Antimicrobial Activity ◽  
Comparative Evaluation ◽  
Mineral Trioxide Aggregate

scholarly journals Proliferation of Antibiotic-Producing Bacteria and Concomitant Antibiotic Production as the Basis for the Antibiotic Activity of Jordan's Red Soils

2009 ◽  
Vol 75 (9) ◽  
pp. 2735-2741 ◽  
Author(s):  
Joseph O. Falkinham ◽  
Thomas E. Wall ◽  
Justin R. Tanner ◽  
Khaled Tawaha ◽  
Feras Q. Alali ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Incubation Period ◽  
Micrococcus Luteus ◽  
Antibiotic Production ◽  
Antibiotic Activity ◽  
Skin Infections ◽  
High Antimicrobial Activity ◽  
Methanol Extracts ◽  
Red Soils

ABSTRACT Anecdotes, both historical and recent, recount the curing of skin infections, including diaper rash, by using red soils from the Hashemite Kingdom of Jordan. Following inoculation of red soils isolated from geographically separate areas of Jordan, Micrococcus luteus and Staphylococcus aureus were rapidly killed. Over the 3-week incubation period, the number of specific types of antibiotic-producing bacteria increased, and high antimicrobial activity (MIC, ∼10 μg/ml) was observed in methanol extracts of the inoculated red soils. Antibiotic-producing microorganisms whose numbers increased during incubation included actinomycetes, Lysobacter spp., and Bacillus spp. The actinomycetes produced actinomycin C2 and actinomycin C3. No myxobacteria or lytic bacteriophages with activity against either M. luteus or S. aureus were detected in either soil before or after inoculation and incubation. Although protozoa and amoebae were detected in the soils, the numbers were low and did not increase over the incubation period. These results suggest that the antibiotic activity of Jordan's red soils is due to the proliferation of antibiotic-producing bacteria.

scholarly journals Identification of Genes Encoding Antimicrobial Proteins in Langerhans Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Aislyn Oulee ◽  
Feiyang Ma ◽  
Rosane M. B. Teles ◽  
Bruno J. de Andrade Silva ◽  
Matteo Pellegrini ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Host Defense ◽  
Langerhans Cells ◽  
Scientific Literature ◽  
Inflammatory Responses ◽  
Microbial Pathogens ◽  
Anatomical Location ◽  
Skin Infections ◽  
Antimicrobial Proteins ◽  
Genes Encoding

Langerhans cells (LCs) reside in the epidermis where they are poised to mount an antimicrobial response against microbial pathogens invading from the outside environment. To elucidate potential pathways by which LCs contribute to host defense, we mined published LC transcriptomes deposited in GEO and the scientific literature for genes that participate in antimicrobial responses. Overall, we identified 31 genes in LCs that encode proteins that contribute to antimicrobial activity, ten of which were cross-validated in at least two separate experiments. Seven of these ten antimicrobial genes encode chemokines, CCL1, CCL17, CCL19, CCL2, CCL22, CXCL14 and CXCL2, which mediate both antimicrobial and inflammatory responses. Of these, CCL22 was detected in seven of nine transcriptomes and by PCR in cultured LCs. Overall, the antimicrobial genes identified in LCs encode proteins with broad antibacterial activity, including against Staphylococcus aureus, which is the leading cause of skin infections. Thus, this study illustrates that LCs, consistent with their anatomical location, are programmed to mount an antimicrobial response against invading pathogens in skin.

Effect of Operator-specific Handling on Tooth-composite Interface and Microleakage Formation

10.2341/08-66 ◽  
2009 ◽  
Vol 34 (2) ◽  
pp. 200-210 ◽  
Author(s):  
H. Schneider ◽  
I. Busch ◽  
M. Busch ◽  
H. Jentsch ◽  
M. Häfer
Keyword(s):  
Clinical Relevance ◽  
Comparative Evaluation ◽  
Interface Morphology ◽  
Etch And Rinse ◽  
Composite Interface ◽  
Bonding Systems

Clinical Relevance After calibration of the restoration procedure, three operators experienced differences in tooth-composite interface morphology and microleakage formation. These differences reflect technique-sensitivity of the etch-and-rinse adhesive. The lowest microleakage on dentin was related to an enhanced interface integrity. After additional application of an adhesive component, sealing of the enamel and dentin was partially enhanced, but microleakage formation did not decrease. These results are helpful in the comparative evaluation of other bonding systems.

scholarly journals Development, Characterization, and Evaluation of Novel Broad-Spectrum Antimicrobial Topical Formulations from Cymbopogon martini (Roxb.) W. Watson Essential Oil

2018 ◽  
Vol 2018 ◽  
pp. 1-16 ◽  
Author(s):  
Negero Gemeda ◽  
Ashenif Tadele ◽  
Hirut Lemma ◽  
Biruktawit Girma ◽  
Getachew Addis ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Broad Spectrum ◽  
Chemical Constituents ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Skin Infections ◽  
Topical Formulations ◽  
Life Study

Background. Skin infections were the most frequently encountered of all infections and the 4th leading cause of nonfatal disease burden. Topical drugs have been used for the management of skin infections. The growing concern of drug resistance to the topical agents has warned the need for continuous development of novel drug. Essential oils are the best candidate for new drug with different mode of action and target as they are rich in chemical constituents. Objective. To evaluate and develop safe and effective topical antimicrobial formulations from essential oil of Cymbopogon martini. Method. Essential oil was extracted using hydrodistillation aerial part C. martini and topical formulations were prepared in five different semisolid bases. In vitro antimicrobial investigations were performed on essential oil and topical formulations. Skin sensitizations of the formulations were evaluated using guinea pig maximization. Results. The essential oil of C. martini has shown broad-spectrum antimicrobial potency against all tested organisms with MIC value ranging from 0.65 to 10 μg/ml. Absolute inhibitions of growth of fungi were observed against Trichophyton mentagrophytes and Trichophyton rubrum at concentrations above 1% of oil and against Microsporum canis and Trichophyton verrucosum at a concentration of 4% oil. Among topical formulations, the highest antimicrobial activity was recorded in hydrophilic ointment followed by macrogol blend ointment. The antimicrobial activity of oil was higher in fungal pathogen compared to bacteria. Gram positive bacteria were more sensitive than gram negative bacteria. Hydrophilic and macrogol blend ointment containing 5% oil did not produce any skin sensitization on guinea pigs. Conclusion. In conclusion, topical formulations of C. martini essential oil can be alternative topical agents with safe broad-spectrum activity for the treatment of skin disorder. Further studies should focus on shelf life study and clinical study of the product.

Comparative evaluation of antimicrobial activity of different types of ionic liquids

2019 ◽  
Vol 104 ◽  
pp. 109907 ◽  
Author(s):  
Walter Florio ◽  
Stefano Becherini ◽  
Felicia D'Andrea ◽  
Antonella Lupetti ◽  
Cinzia Chiappe ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ionic Liquids ◽  
Comparative Evaluation ◽  
Different Types

scholarly journals Comparative Evaluation of Antimicrobial Activity and Composition of Rose Oils from Various Geographic Origins, in Particular Bulgarian Rose Oil

2008 ◽  
Vol 3 (7) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Velizar Gochev ◽  
Katrin Wlcek ◽  
Gerhard Buchbauer ◽  
Albena Stoyanova ◽  
Anna Dobreva ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Essential Oils ◽  
Bacillus Cereus ◽  
Phenolic Compound ◽  
Comparative Evaluation ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Geographic Origins ◽  
Rose Oil

In the present study we evaluated the composition and antimicrobial activity of various rose oils from Bulgaria, Turkey, Morocco, Iran and China against three Gram-positive, three Gram-negative bacteria and two yeasts. The composition of the studied essential oils was determined by GC and GC/MS. Citronellol was the major compound of all the oil samples: 31.7% (Chinese oil); 32.6% (Iranian oil); 33.6% (Moroccan oil); 34.9% (Bulgarian oil) and 38,7 % (Turkish oil). For the Bulgarian oil we could determine the highest activity against all microorganisms. Bacillus cereus ATCC 11778 was the most sensitive strain to Bulgarian rose oil (MCC 128 μg/mL) and Pseudomonas aeruginosa ATCC 9627 and P. fluorescens were more resistible strains (MCC 4096 μg/mL). Antimicrobial activity of rose oils is mainly due to the action of oxygenated acyclic monoterpenes citronellol, geraniol, nerol and linalool, their acetate derivatives and the phenolic compound eugenol.

Sign in / Sign up

Export Citation Format

Share Document