scholarly journals Cytokinins and urea derivatives stimulate seed germination in Lotus corniculatus L.

2007 ◽  
Vol 59 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Radomirka Nikolic ◽  
Nevena Mitic ◽  
Suzana Zivkovic ◽  
D. Grubisic ◽  
Mirjana Neskovic
Keyword(s):  
Seed Germination ◽  
Lotus Corniculatus ◽  
Urea Derivatives ◽  
Adenine Sulfate ◽  
Germination Potential ◽  
Aged Seed ◽  
Isopentenyl Adenine ◽  
Aromatic Cytokinins ◽  
Aged Seeds

We studied the effects of various cytokinins and urea derivatives on germination of aged seeds of in Lotus corniculatus L. The following substances were applied: N6-isoprenoid cytokinins (isopentenyl adenine and zeatin), adenine sulfate, N6-aromatic cytokinins (kinetin, benzyladenine) and their N9-ribosides, N-benzyl-9-(2- tetrahydropyranyl)adenine, and urea derivatives (diphenylurea, thidiazuron, and chloro-pyridyl phenylurea). With the exception of adenine sulfate, all cytokinins increased the percentage of seed germination up to twofold, depending on their kind and concentration. It is concluded that cytokinins may be among the missing factors in aged seeds of L. corniculatus contributing to the implementation of their full germination potential. They could be used to improve germination of both freshly harvested and aged seed samples, if necessary. .

scholarly journals A simple new method for aged seed utilization based on melatonin-mediated germination and antioxidant nutrient production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Aged Seed ◽  
Phenolics Content ◽  
Dpph Radical Scavenging ◽  
Aged Seeds

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.

RAPID DETERMINATION OF SEED GERMINATION POTENTIAL TO MEASURE QUALITY LOSS IN STORED WHEAT

1982 ◽  
Vol 62 (4) ◽  
pp. 1045-1048 ◽  
Author(s):  
N. D. G. WHITE ◽  
R. N. SINHA ◽  
W. E. MUIR
Keyword(s):  
Seed Germination ◽  
Moisture Content ◽  
Rapid Method ◽  
Rapid Determination ◽  
Quality Loss ◽  
Wheat Seed ◽  
Stored Wheat ◽  
Germination Potential

A rapid method of determining wheat seed germination after 1 day (G1) of incubation was found. Germination of wheat seed stored at [Formula: see text] moisture content is estimated by adding 12% to G1; at <17% moisture content germination is not consistently predicted by G1.

Changes of enzymes activities in botanical treated aged seed of soybean (Glycine max (L.) Merrill) cv. CO 3 seeds

2017 ◽  
Author(s):  
Hridya V. Rejeendran ◽  
S. Lakshmi ◽  
S. Ambika
Keyword(s):  
Enzyme Activities ◽  
Deleterious Effect ◽  
Seed Quality ◽  
Accelerated Ageing ◽  
Fenugreek Seed ◽  
Aged Seed ◽  
Glycine Max L ◽  
Field Emergence ◽  
Nano Size ◽  
Aged Seeds

Changes occurring in seed during aging are very significant for determining seed quality, i.e. seed longevity. An experiment was conducted in Department of Seed Science and Technology, TNAU, Coimbatore to standardize suitable seed dry dressing treatments, using near nano size botanicals that can alleviate the deleterious effect of accelerated ageing and to improve the enzyme activities of soybean aged seeds. Five days aged seeds were treated with 1 h ball milled fenugreek seed powder, leaf powders of ashwagandha, tea and noni @ 2 g kg-1 with 60 min. shaking and evaluated for enzyme activities and field emergence (naturally and artificially aged seeds). Among the treatments seeds treated with fenugreek seed powder has the highest dehydrogenase activity (OD 10 min-1), protein (%), oil content (%), catalase activity, peroxidase activity (OD 10 min-1) (µg H2O2 mg-1min-1) with minimum values of electrical conductivity (dSm-1), free fatty acid (%), lipid peroxidation (OD value), lipoxygenase activity (g mol s-1 mg-1). Field emergence (%) was also higher in fenugreek seed powder treated seeds (2 g kg-1 with 60 min).

Effects of Cytokinins on In Vitro Seed Germination and Early Seedling Morphogenesis in Lotus corniculatus L.

2006 ◽  
Vol 25 (3) ◽  
pp. 187-194 ◽  
Author(s):  
Radomirka Nikolić ◽  
Nevena Mitić ◽  
Rade Miletić ◽  
Mirjana Nešković
Keyword(s):  
Seed Germination ◽  
Lotus Corniculatus ◽  
Early Seedling

scholarly journals The Effects of Temperature, Light and Moisture on the Seed Germination of Siphonostegia chinensis Benth.

2019 ◽  
Vol 1 (2) ◽  
Author(s):  
Yongji Wang
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Germination Index ◽  
High Concentration ◽  
Complete Darkness ◽  
Germination Potential ◽  
Medium Level ◽  
Different Temperatures ◽  
High Level ◽  
Germination Experiment

To explore the optimum temperature, light intensityand water conditions for seed germination of Siphonostegia chinensis Benth.,seed germination experiment were carried out under different temperatures（5/15, 10/20, 15/25, 20/30℃）, different light intensity（14h light/10h darkness, complete darkness）and different concentrations（0％, 5％, 10％, 15％, 20％）of PEG-6000 solution. In terms of concentration, 5% PEG was regarded as the low level, 10% and 15% as the medium level, and 20% as the high level. The results showed that (1) Germination rate, germination potential, and germination index were increased with the rise of temperature. In addition, seed germination was significantly higher under the dark conditions than that with the 14h light/10h darkness. (2) No seed germination occurred when the temperature was below 10/20 ºC at 14h light/10h darkness. (3) Under 14h light/10h darkness, the germination rate, germination potential and germination index first increased and then decreased with the increase of PEG concentration. The low concentration was more beneficial to the seed germination. (4) Under the condition of complete darkness, the germination rate, germination potential and germination index decline with fluctuation with the increase of PEG concentration. Seed germination of Siphonostegia chinensis Benth. was inhibited in high concentration of PEG.

scholarly journals The Opposite Roles of White Light in Regulating Germination of Fresh and Aged Seed in Tobacco

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2457
Author(s):  
Yao Wang ◽  
Min Zhang ◽  
Shuai Dong ◽  
Yi-Ling Liu ◽  
Zhen-Hua Li
Keyword(s):  
Abscisic Acid ◽  
Environmental Factors ◽  
White Light ◽  
Plant Hormones ◽  
Natural Environment ◽  
Aged Seed ◽  
Aged Seeds

Light is one of the important environmental factors for seeds to evaluate whether the natural environment is appropriate for germination and subsequent seedlings emergence. The mechanism of light-mediated germination is mainly concerned with fresh seeds (FS) of model plants but is poorly understood in aged seeds. Here, the effects of light on germination of FS and naturally aged seeds (NAS) in tobacco and their relationship with plant hormones gibberellins (GA) and abscisic acid (ABA) were investigated. The results demonstrated that light promoted and inhibited the germination of FS and NAS, respectively. GA and ABA were involved in the germination control of NAS, as well as in FS. However, light suppressed GA signal and stimulated ABA signal in NAS, whereas it stimulated GA signal and suppressed ABA signal in FS. In addition, light stimulated the GA accumulation and reduction in ABA in FS while inhibiting the increase in GA level in NAS. Together, the present study demonstrates that light has opposite effects on the germination of FS and NAS, which are closely related to the metabolism and/or signaling of plant hormones ABA and GA.

scholarly journals Effects of Temperature on Seed Germination of Invasive Plant Rorippa amphbia (L.) Besser

2019 ◽  
pp. 1-7
Author(s):  
Yufeng Xu ◽  
Miao Liu ◽  
Yuanhao Lv ◽  
Qiuli Wang ◽  
Bo Qu ◽  
...  
Keyword(s):  
Global Warming ◽  
Seed Germination ◽  
Filter Paper ◽  
Invasive Plant ◽  
Germination Rate ◽  
Petri Dish ◽  
Liaoning Province ◽  
Germination Index ◽  
Temperature Range ◽  
Germination Potential

Aims: Global warming and biological invasion are major environmental issues faced in the world. In the study, Rorippa amphibia, a perennial invasive clone plant in northern China, was used as a material to study the germination characteristics of the seeds at different temperatures. Study Design: Germination test of R. amphibia seeds at different temperature was studied by means of laboratory culture. The germination percentage, germination index, germination potential, bud height and root length of the seeds were determined. Place and Duration of Study: Samples were collected from the west side of the swimming pool of Shenyang Agricultural University of Liaoning Province in August 2017. Experiments were done in the College of Biological Science and Technology, between October 2017 and June 2018. Methodology: The petri dish method was used in the experiment. Fifty seeds were randomly selected and soaked in distilled water for 12h. The seeds were placed in a petri dish covered with double filter paper, cultured at 10℃, 15℃, 20℃, 25℃, 30℃, 35℃ and 40℃ in light incubators for 12h darkness and 12h light (4000lux), with 3 repeats per processing. Seed germination was based on embryo root breakthrough seed coat ≥ 1 mm. During the experiment, the numbers of seed germination were recorded every day, and the filter paper was kept moist until there was no new seed germination for 2 consecutive days, which was regarded as the end of germination. The numbers of seed germination should be counted regularly every day, and the beginning and duration of germination should be recorded. The germination rate, daily germination rate, germinating potential, germinating index and vigor index of R. amphibia seeds at different temperature were calculated by measuring bud height and radicle length on the 10th day after germination. Results: The temperature range of seed germination of R. amphibia was wide, which could germinate at 15 - 40℃. Lower temperature delayed the peak period of seed germination at some extent and the germination rates of R. amphibia peak at 30 - 35℃, which were 44.67% and 50% respectively. At 35℃, germination potential and germination index were 25.33% and 29.46, reaching the maximum value. Conclusion: The reason for the wide temperature range of seed germination and the low germination rate might be the candidate method for clonal plant population establishment in temperate zone. The higher germination rate of high temperature condition suggested that clone invasive plants in temperate regions were more invasive during global warming.

EFFECT OF SEED SIZE ON GERMINATION, SEEDLING VIGOR, ELECTROLYTE LEAKAGE, AND ESTABLISHMENT OF BIRD’S-FOOT TREFOIL (Lotus corniculatus L.)

1981 ◽  
Vol 61 (2) ◽  
pp. 337-343 ◽  
Author(s):  
B. D. McKERSIE ◽  
D. T. TOMES ◽  
S. YAMAMOTO
Keyword(s):  
Seed Size ◽  
Electrolyte Leakage ◽  
Seed Weight ◽  
Seed Viability ◽  
Lotus Corniculatus ◽  
Seedling Vigor ◽  
Germination Potential ◽  
Seedling Length ◽  
Percent Germination ◽  
Highly Correlated

Seed viability, seedling vigor, and field establishment were measured on five seed sizes from five commercial, Certified seed lots of bird’s-foot trefoil (Lotus corniculatus L. ’Leo’). Viability as determined by percent germination at 20 °C varied among seed lots and among seed sizes, but a consistent relationship between viability and seed size was not observed. Similarly, speed of germination did not vary in response to seed size but did vary among lots. Within a seed lot, seedling length after 7 days growth varied in response to seed size; the larger seeds produced longer seedlings. However, this relationship was not observed among seed lots because seed lots with the highest average seed weight did not produce the longest seedlings. The seedling vigor ranking on the basis of electrolyte leakage differed if leakage was expressed relative to seed weight or the seed number. Leakage per 100 seeds was significantly and negatively correlated with percent germination, but not with seedling length or field establishment. On the other hand, leakage per gram of seed was significantly and negatively correlated with percent germination, seedling length, and field establishment. The larger seed sizes within a lot tended to have improved field establishment, so field establishment was highly correlated with seedling length (r = 0.76). Electrolyte leakage per gram of seed, and percentage germination were also significantly correlated with establishment (r = − 0.59 and 0.45, respectively). Germination potential and ability of the seedling to elongate rapidly are related to the field establishment of bird’s-foot trefoil. Germination potential was primarily influenced by the seed lot, whereas the ability to elongate was influenced by seed size (within a seed lot).

scholarly journals Tolerance against ageing in different varieties of Brassica rapa var toria, B. rapa var yellow sarson and B. juncea( L.)

2018 ◽  
Vol 10 (1) ◽  
pp. 171-177
Author(s):  
Bhim Jyoti ◽  
Rambhajan Rambhajan ◽  
Ushapant Ushapant
Keyword(s):  
Developing Countries ◽  
Seed Germination ◽  
Laboratory Condition ◽  
Analysis Of Variance ◽  
Brassica Rapa ◽  
Seed Viability ◽  
Seed Deterioration ◽  
Brassica Spp ◽  
Aged Seed ◽  
Ageing Period

Seed deterioration is a serious problem in developing countries where seeds are stored in places usually without a proper control of humidity and temperature. In this investigation, an effort was made to identify crops as well as varieties of brassica spp. tolerance to ageing and predicting loss of seed viability at regular interval through standard germination under laboratory condition. Analysis of variance showed that mean squares due to crops, ageing periods and crop× ageing period were significant for % seed germination. Result revealed that standard germination declined from 95.63 to 37.54% in toria, 92 to 28.96 in yellow sarson, and 93.79 to 30.92% in mustard. Similar changes in germination % were observed at 9 and 12 months of observation. After 6 months, germination % declined from 95.13 to 35.50% in toria, 91.87 to 28% in yellow sarson and 93.33 to 29% in mustard. Analysis of variance showed significant differences for %seed germination due to varieties, ageing period and interaction of varieties × ageing periods. At varietal level AP2, AP3 and AP4 aged seed, Bhawani (82.66%, 68.16% and 28.17%) showed higher seed germination % respectively than other three varieties of toria. In case of yellowsarson and mus-tard least deterioration for standard germination were observed in B-9 and Kranti respectively.This study indicates that toria seeds have better storability than yeloowsarson and mustard. At varietal level good storage potential was found in Bhawani, B-9 and Krantivariety of toria, yellow sarson and mustard respectively than other three varieties of studied crops.

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