Changes of enzymes activities in botanical treated aged seed of soybean (Glycine max (L.) Merrill) cv. CO 3 seeds

2017 ◽  
Author(s):  
Hridya V. Rejeendran ◽  
S. Lakshmi ◽  
S. Ambika
Keyword(s):  
Enzyme Activities ◽  
Deleterious Effect ◽  
Seed Quality ◽  
Accelerated Ageing ◽  
Fenugreek Seed ◽  
Aged Seed ◽  
Glycine Max L ◽  
Field Emergence ◽  
Nano Size ◽  
Aged Seeds

Changes occurring in seed during aging are very significant for determining seed quality, i.e. seed longevity. An experiment was conducted in Department of Seed Science and Technology, TNAU, Coimbatore to standardize suitable seed dry dressing treatments, using near nano size botanicals that can alleviate the deleterious effect of accelerated ageing and to improve the enzyme activities of soybean aged seeds. Five days aged seeds were treated with 1 h ball milled fenugreek seed powder, leaf powders of ashwagandha, tea and noni @ 2 g kg-1 with 60 min. shaking and evaluated for enzyme activities and field emergence (naturally and artificially aged seeds). Among the treatments seeds treated with fenugreek seed powder has the highest dehydrogenase activity (OD 10 min-1), protein (%), oil content (%), catalase activity, peroxidase activity (OD 10 min-1) (µg H2O2 mg-1min-1) with minimum values of electrical conductivity (dSm-1), free fatty acid (%), lipid peroxidation (OD value), lipoxygenase activity (g mol s-1 mg-1). Field emergence (%) was also higher in fenugreek seed powder treated seeds (2 g kg-1 with 60 min).

scholarly journals Comparative analysis of the Accelerated Aged seed transcriptome profiles of maize CSSLs (I178 and X178)

2019 ◽  
Author(s):  
Li Li ◽  
Feng Wang ◽  
Xuhui Li ◽  
Yixuan Peng ◽  
Hongwei Zhang ◽  
...  
Keyword(s):  
Seed Quality ◽  
Seed Longevity ◽  
Accelerated Ageing ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Carbohydrate Derivative ◽  
Significant Difference ◽  
Seed Ageing ◽  
Aged Seed ◽  
Segment Substitution

AbstractSeed longevity is one of the most essential characters of seed quality. Two Chromosome segment substitution lines (CSSL) I178 and X178 with significant difference on seed longevity were subjected to transcriptome sequencing before (0d-AA) and after five days of accelerated ageing (5d-AA) treatments. Compared to the non-accelerated ageing treatment (0d-AA), 286 and 220 differential expressed genes (DEGs) were identified in I178 and X178, respectively Among those, 98 DEGs were detected in both I178 and X178 after 5d-AA, Enriched GO terms included cellular components of cell part, intracellular part, organelle and membrane etc., including carbohydrate derivative catabolic process, carbohydrate synthesis, sugar isomerase (SIS) family protein etc. Transcriptome analysis of I178 and X178 showed that Alternative splicing (AS) occurs in 63.6% of the expressed genes in all samples. Only 381 genes specifically occurred AS in I178 and X178 after 5d-AA, mostly enriched in nucleotide and nucleoside binding. Combined with the reported QTL mapping result, the DEG and the AS information, 13 DEGs in the mapping intervals and 7 AS-DEGs were potential candidates may directly or indirectly associated to seed ageing.

Dry dressing of soybean [Glycine max (L.) Merrill] cv. CO 3 seeds with near nano size botanicals to improve physiological performance during storage

2017 ◽  
Author(s):  
V. R. Hridya ◽  
S. Lakshmi ◽  
S. Ambika
Keyword(s):  
Glycine Max ◽  
Biochemical Parameter ◽  
Physiological Parameters ◽  
Monthly Interval ◽  
Ambient Conditions ◽  
Fenugreek Seed ◽  
Matter Production ◽  
Vigour Index ◽  
Glycine Max L ◽  
Nano Size

The effect of seed treatment with botanicals was evaluated on storability of soybean (Glycine max (L.) Merrill). Seeds treated with fenugreek seed powder, leaf powders of ashwagandha, tea and noni (ball milled for 1 h) @ 2 g kg-1 with 60 min shaking and stored in cloth bag for 6 months under ambient conditions. Seeds were evaluated for physiological parameters such as speed of germination, germination per cent, root length, shoot length, vigour index and dry matter production and for biochemical parameter the electrical conductivity at monthly interval. Results revealed that seed dressing with fenugreek seed powder manifested the physiological parameters at higher order with lesser electrical. Hence seed dry dressing with fenugreek seed powder obtained on ball milling for 1 h @ 2g per kg of seed maintained the germination and vigour in storage .

Estimation of total phenolics in soybean (Glycine max L.) exudates and seed quality during accelerated ageing

2005 ◽  
Vol 33 (3) ◽  
pp. 761-765
Author(s):  
A. Simić ◽  
S. Sredojević ◽  
M. Todorović ◽  
L. Đukanović ◽  
M. Damjanović
Keyword(s):  
Glycine Max ◽  
Seed Quality ◽  
Total Phenolics ◽  
Accelerated Ageing ◽  
Glycine Max L

scholarly journals A simple new method for aged seed utilization based on melatonin-mediated germination and antioxidant nutrient production

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Song Yu ◽  
Xuetian Zhu ◽  
Helin Yang ◽  
Lihe Yu ◽  
Yifei Zhang
Keyword(s):  
Total Phenolics ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Aged Seed ◽  
Phenolics Content ◽  
Dpph Radical Scavenging ◽  
Aged Seeds

AbstractSeed deterioration, coupled with a decrease in nutrients, is unavoidable following long-term storage, and these seeds are therefore used as livestock fodder. Here, we developed a simple, rapid and efficient method of producing high amounts of antioxidants from deteriorated seeds via melatonin-induced germination. Legume seeds were subjected to high humidity at 55 °C for 12–36 h to obtain aged seeds with a 40% germination rate and severely reduced antioxidant nutrition (total phenolics content, ferric reducing power and 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging capacity). Aged seeds were then treated with 0.1 mM melatonin, resulting in the production of sprouts with a higher total phenolics content (fivefold), greater ferric reducing power (sevenfold) and greater DPPH radical scavenging capacity (twofold) compared to the aged seeds. These findings suggest that melatonin treatment efficiently converted aged seed reserve residues into antioxidant nutrients, providing an alternative use for deteriorated seeds in food production.

scholarly journals Cytokinins and urea derivatives stimulate seed germination in Lotus corniculatus L.

2007 ◽  
Vol 59 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Radomirka Nikolic ◽  
Nevena Mitic ◽  
Suzana Zivkovic ◽  
D. Grubisic ◽  
Mirjana Neskovic
Keyword(s):  
Seed Germination ◽  
Lotus Corniculatus ◽  
Urea Derivatives ◽  
Adenine Sulfate ◽  
Germination Potential ◽  
Aged Seed ◽  
Isopentenyl Adenine ◽  
Aromatic Cytokinins ◽  
Aged Seeds

We studied the effects of various cytokinins and urea derivatives on germination of aged seeds of in Lotus corniculatus L. The following substances were applied: N6-isoprenoid cytokinins (isopentenyl adenine and zeatin), adenine sulfate, N6-aromatic cytokinins (kinetin, benzyladenine) and their N9-ribosides, N-benzyl-9-(2- tetrahydropyranyl)adenine, and urea derivatives (diphenylurea, thidiazuron, and chloro-pyridyl phenylurea). With the exception of adenine sulfate, all cytokinins increased the percentage of seed germination up to twofold, depending on their kind and concentration. It is concluded that cytokinins may be among the missing factors in aged seeds of L. corniculatus contributing to the implementation of their full germination potential. They could be used to improve germination of both freshly harvested and aged seed samples, if necessary. .

scholarly journals Effect of Priming on Physiological Seed Quality in Fresh and Aged Seeds of Sunflower (Helianthus annuus L.) Hybrid KBSH-53

2018 ◽  
Vol 7 (04) ◽  
pp. 2087-2095
Author(s):  
B. Manjunatha ◽  
B.C. Channakeshava ◽  
Y.G. Shadakshari ◽  
K. Bhanuprakash ◽  
K.R. Sreeramulu ◽  
...  
Keyword(s):  
Helianthus Annuus ◽  
Seed Quality ◽  
Helianthus Annuus L ◽  
Aged Seeds

scholarly journals Accelerated aging and seedling field emergence in soybean

2004 ◽  
Vol 61 (5) ◽  
pp. 476-480 ◽  
Author(s):  
Reynaldo Melo Torres ◽  
Roberval Daiton Vieira ◽  
Maristela Panobianco
Keyword(s):  
Environmental Conditions ◽  
Close Association ◽  
Accelerated Aging ◽  
Field Performance ◽  
Accurate Estimate ◽  
Seed Vigor ◽  
Soybean Seedlings ◽  
Physiological Quality ◽  
Glycine Max L ◽  
Field Emergence

Information regarding the relationship between laboratory seed vigor testing and seedling field emergence is very important to estimate seed performance after sowing and help producers adopt the best procedures to improve stand establishment. The objective of the present study was to relate accelerated aging (AA) test results to soybean [Glycine max (L.) Merrill] seedling field emergence results based on data obtained over three agricultural years. The following evaluations were made: seed water content, standard germination, accelerated aging, electrical conductivity, and seedling field emergence. There was a close association between planting environmental conditions, seed physiological quality and seedling field emergence. The most accurate predictions were obtained for AA values > 90%, when field emergence was higher than 80% (r² = 0.90). Based on the results it was concluded that the AA test provided an accurate estimate of field emergence of soybean seedlings. However, as seedbed environmental conditions became less favorable, the ability of the AA test to estimate field performance significantly decreased.

scholarly journals Detection of QTL underlying seed quality components in soybean [Glycine max (L.) Merr.]

2018 ◽  
Vol 98 (4) ◽  
pp. 881-888 ◽  
Author(s):  
Masum Akond ◽  
Jiazheng Yuan ◽  
Shiming Liu ◽  
Stella K. Kantartzi ◽  
Khalid Meksem ◽  
...  
Keyword(s):  
Amino Acid ◽  
Phenotypic Variation ◽  
Seed Quality ◽  
Snp Markers ◽  
Seed Composition ◽  
Single Nucleotide ◽  
Glycine Max L ◽  
Amino Acid Contents ◽  
N 93 ◽  
Major Qtl

Improving seed composition and quality, including protein, oil, fatty acid, and amino acid contents, is an important goal of soybean farmers and breeders. The aim of this study was to map the quantitative trait loci (QTL) underlying the contents of protein, oil, fatty acids, and amino acids with 1510 single nucleotide polymorphism (SNP) markers using the ‘Hamilton’ × ‘Spencer’ recombinant inbred line population (H × S; n = 93). A total of 13 QTL for the traits studied have been mapped on 3 chromosomes (Chr.) of the soybean genome. Three major QTL have been mapped to a 7–13 cM region on Chr. 6. One major QTL for oil content (qOIL001) explained approximately 76% of the total phenotypic variation in this population; the second major QTL for amino acid alanine (Ala; qALA001) explained approximately 74% of the total variation in Ala content; moreover, two major QTL for palmitic acid (qPAL001 and qPAL002) were identified on Chr. 6 and explained approximately 21% of the phenotypic variation in this population. The SNP markers flanking the QTL identified here will be very useful for soybean breeders to develop and select soybean lines with higher seed composition qualities using marker-assisted selection.

scholarly journals Induction of resistance and chemical management of mildew and Asian rust in soybean cultivation

2020 ◽  
Vol 68 (4) ◽  
pp. 285-290
Author(s):  
Daniel Claudio Grigolo ◽  
Jean Carlo Possenti ◽  
Marcieli Da Silva ◽  
Sergio Miguel Mazaro ◽  
Karina Guollo
Keyword(s):  
Yield Components ◽  
New Technologies ◽  
Seed Quality ◽  
Soybean Rust ◽  
Soybean Seeds ◽  
Chemical Management ◽  
Lost Productivity ◽  
Glycine Max L ◽  
Induction Of Resistance

Soybean (Glycine max (L.) Merrill) is one of the most cultivated in Brazil and has lost productivity due to the attack of disease pests. Thus, it is necessary to develop new technologies capable of reducing damages caused by such biological agents. The aim of this work was to evaluate whether the chemical management of ‘eyespot’ and the induction of resistance through the use of phosphites and acibenzolar-s-methyl (ASM), in association with commercial fungicides, affect the quality of soybean seeds. The study was conducted in the field, during two consecutive harvests in Dois Vizinhos, Paraná, Brazil, and a randomized blocks experimental designs was used. The response-variables evaluated were yield components, disease severity through specific diagrammatic scales, and seed quality attributes. The use of phosphite and ASM, with and without the combination of fungicides Glifosato, Azoxistrobina + Benzovindiflupir, Azoxistrobina + Ciproconazol and Picoxistrobina + Ciproconazol did not reduce the severity of the mildew and asian soybean rust. The use of combinations of different fungicides was sufficient to mitigate the severity effects of the mildew and asian rust on soybean crop.

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