PROSPECTS OF OBTAINING RAW MATERIALS BY METHODS OF BIOTECHNOLOGY AND SCREENING OF THE CHEMICAL COMPOSITION OF BIOMASS IRIS SPURIA L. (ART AND SOUL)

Hydroponic technologies combined with clonal micropropagation have the potential for large-scale plant growth and production of secondary metabolites. The aim of this study was to obtain raw materials Iris spuria L. (Art And Soul) in hydroponics coupled with clonal micropropagation, and its primary pharmacognostic analysis. When receiving the raw material Iris spuria L. (Art And Soul) at the stage of micro-multiplication itself, the most optimal content in the nutrient medium was 2.5-5.0 µm BAP. For a more complete realization of morphogenetic potentials it is necessary to alternate media with the content of phytohormones and hormone-free. At the same time, l-glutamine and adenine sulfate in an amount of 100 mg/l (MS+100mg/l L-glutamine+100mg/l adenine sulfate) should be added to the hormone-free media. For the adaptation of regenerated plants to non-sterile conditions and in the cultivation of raw materials can be used universal three-tiered aeroponic system, developed by All-Russian Research Institute of Agricultural Biotechnology, Moscow (Russia) (Iu.Ts. Martirosian). The obtained biotechnological raw materials (grass) were identified by macroscopic (external) and microscopic (anatomical) features (in accordance with the requirements of the State Pharmacopoeia of the Russian Federation XIV. 2018). As a result of the qualitative analysis of raw materials for the content of the main groups of biologically active substances, the following were revealed: phenols, flavonoids, tannins, alkaloids, glycosides, xanthones. For the herb Iris spuria L. the method of quantitative determination of flavonoids in terms of quercetin is developed. The dependence of the accumulation of quercetin and tannins on the hormonal composition of nutrient media, which makes it possible for Iris spuria L. (Art And Soul) to regulate the accumulation of these polyphenols in the production of plant raw materials.

IN VITRO BREEDING AND NURSING TAQUA BANANA IN TRA VINH PROVINCE

The goal of this study is to determine in vitro propagation media of Taqua banana. The results showed that: the optimal medium for banana bud regeneration was MS medium (Murashine & Skoog 1962) supplemented 0.1 mg/l NAA, 100 mg/l adenine sulfate, 30g/l sucrose, 8 g/l Agar, 5 mg/l BAP, 10% volume coconut juice and kept in completely dark condition. The medium, which is similar to bud generation media except for supplementing BAP 7 mg/l, was also good for bud replication with 6,33 shoots per sample after 4 weeks. (2)The medium, which is similar to bud generation media except for non-added BAP and an increase of NAA from 0.1 to 1 mg/l, was the best for the banana rooting.

Penggunaan Adenin Sulfat Pada Perbanyakan Mikro Talas Jepang

Taro is a staple food source in several regions of Indonesia. One type of this crop, namely Japanese taro or satoimo [Colocasia esculenta (L.) Schott var. antiquorum], is widely consumed in Japan so that there are export opportunities into the country. To overcome the problem of lack of propagules in the cultivation of taro, quality seed production can be done with micro propagation. This study aimed to obtain MS tissue culture media with the right concentration of adenine sulfate for Japanese taro micro propagation. The study was designed as a single factor experiment with a Completely Randomized Design, with an experimental factors of adenine sulfate concentration consisting of 5 levels, namely: A0 (control, 0 mg / l), A1 (10 mg / l), A2 (60 mg / l ), A3 (110 mg / l), A4 (160 mg / l), and with 5 replications. The use of MS media with BAP 0.5 mg /l + adenine sulfate 160 mg/l gave the best results for shoot multiplication with an average number of shoots produced 21.40 per culture bottle, at 12 weeks after culturing. The success percentage of plantlet acclimatization was 86%, and tissue cultur propagules subsequently was able to grow normally under ex vitro conditions.

In vitro Shootlets Multiplication and Callus Proliferation of Jojoba (Simmondsia chinensis) Plant

A protocol for in vitro multiplication and callus proliferation of jojoba was developed. Nodal segments were cultured on MS supplemented with 1 mg/l BA. For multiplication, combination of 1.5 mg/l BA + 1 mg/l IAA resulted in greater number of shootlet. While, 1 mg/l BA + NAA enhanced the height of shootlet and number of nodes. Also, the effect of adenine sulfate and TDZ on shootlets multiplication was examined. The maximum number of shootlets emergence was obtained with medium contained 30 mg/l adenine sulfate. Likewise, the used levels of TDZ specially 2 mg/l enhanced shootlets proliferation as well as length of shootlet and number of nodes. Callus induction using combinations of NAA or picloram with BA or Kn was evaluated. The maximum callus frequency was observed with 5 mg/l BA + 1 mg/l picloram followed by 5 mg/l BA + 2 mg/l picloram containing medium. It was found that fresh mass and growth value increased gradually till the third subculture. Plant Tissue Cult. & Biotech. 28(2): 201-214, 2018 (December)

Effects of Additives in Shoot Multiplication and Genetic Validation in Swertica chirayita Revealed through RAPD Analysis

Enhanced in vitro caulogenesis has been tested in Swertia chirayita on MS supplemented with BAP, IAA, IBA, NAA and additives like adenine sulfate and d?glutamine with 2.5% sucrose. The best in vitro caulogenesis was observed in MS fortified with 4.43 ?M BAP in combination with IAA (0.8 ?M) that resulted increase in shoot multiplication rate. The multiplication and elongation of shoots were further enhanced by the addition of adenine sulfate (0.007%) that resulted in the further increase in multiplication fold. The addition of adenine sulfate reduced the use of other cytokinins with different auxins reported in the previous studies on Swertia chirayita. The study suggests adenine sulfate as a primary assimilable reduced nitrogen source for enhancing the shoot multiplication and elongation in Swertia chirayita. RAPD markers were employed to check the genetic variation among the clonal stock that resulted in 97% similarity.Plant Tissue Cult. & Biotech. 23(1): 11?19, 2013 (June) DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15555