scholarly journals Validation of a new cellulose acetate membrane named 'SELECA-VSP' for electrophoretic fractionation of serum protein: Collaborative study to evaluate lot-to-lot variation and inter-laboratory comparison.

2007 ◽  
Vol 51 (2) ◽  
pp. 135-145
Author(s):  
Hiroko Watanabe ◽  
Aya Sugiura ◽  
Kazuko Ohtake ◽  
Teruko Ohtake ◽  
Hiroko Fukada ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Collaborative Study ◽  
Cellulose Acetate Membrane ◽  
Electrophoretic Fractionation

Electrophoretic fractionation of 5'-nucleotidase

1994 ◽  
Vol 40 (2) ◽  
pp. 190-196 ◽  
Author(s):  
M Panteghini
Keyword(s):  
Cellulose Acetate ◽  
Electrophoretic Pattern ◽  
Hepatobiliary Disease ◽  
Cellulose Acetate Membrane ◽  
Electrophoretic Fractionation

Abstract Human isonucleotidases were separated by electrophoresis on a cellulose acetate membrane. Three 5'-nucleotidase forms, NTP1, NTP2, and NTP3, were resolved with this method and quantified by densitometry. The procedure was not only simple and rapid but also sufficiently precise (between-run CV < 20%) and sensitive (detected nucleotidase fractions of > 0.5 U/L). The effects of various treatments (heat, neuraminidase, glycosidases, proteases, lectins, and detergents) on the electrophoretic pattern of 5'-nucleotidase were studied. NTP1 (mean 12% of total 5'-nucleotidase, SD 5%), NTP2 (mean 30%, SD 8%), and NTP3 (mean 58%, SD 8%) were found in all normal persons studied. The increase in total 5'-nucleotidase in patients with hepatobiliary disease was mainly due to the NTP1 isoform.

THE CONSTRUCTION OF AN ULTRAFILTRATION UNIT TO MEASURE THE BINDING OF CERTAIN NITROGEN MUSTARDS TO SERUM PROTEIN

1962 ◽  
Vol 40 (1) ◽  
pp. 137-146 ◽  
Author(s):  
J. H. Linford ◽  
J. Legal ◽  
E. Zacharias
Keyword(s):  
Blood Serum ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Human Blood ◽  
Human Blood Serum ◽  
Cellulose Acetate Membrane ◽  
Nitrogen Mustards ◽  
Benzene Rings ◽  
High Degree

An ultrafiltration unit has been designed, and constructed to contain a 10-ml volume. The unit is leakproof, is self-contained, and can be operated inside a small refrigerator or oven. It has been used with "Visking" cellulose acetate membrane to separate the colloidal protein phase of human blood serum from the aqueous dispersing phase, and to study the distribution of certain nitrogen mustards between these phases. The results indicate that these drugs are adsorbed to the protein to a high degree and that the presence of the benzene rings in the nitrogen mustards is mainly responsible for this adsorption.

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