Automation of serum protein electrophoresis on cellulose acetate membrane

2016 ◽  
Vol 60 (1) ◽  
pp. 19-22
Author(s):  
Hideki Seo
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Cellulose Acetate Membrane

Underestimation of monoclonal proteins by serum protein electrophoresis on cellulose acetate.

1987 ◽  
Vol 33 (1) ◽  
pp. 182-184 ◽  
Author(s):  
F J Liu ◽  
H A Fritsche ◽  
J M Trujillo
Keyword(s):  
Cellulose Acetate ◽  
Serum Sample ◽  
Serum Protein ◽  
Protein Concentration ◽  
Protein Electrophoresis ◽  
Response To Therapy ◽  
Serum Protein Electrophoresis ◽  
M Protein ◽  
Albumin Concentration ◽  
Undiluted Serum

Abstract Our study of 95 serum samples from 37 patients with monoclonal gammopathy revealed distorted irregular monoclonal (M) protein bands after serum protein electrophoresis (SPE) on cellulose acetate membrane. In 71 (75%) of the 95 sera, the M-protein was underestimated and the albumin concentration overestimated. Dilution of the serum sample before SPE eliminated the abnormality of the M-protein bands. By SPE, the mean albumin concentration in these 71 undiluted sera was 45.8 (SD 7.4) g/L vs 37.9 (SD 5.8) g/L for the diluted sera; moreover, this was true of individual samples: measured albumin concentration in each diluted serum sample was always less than in the undiluted serum. As measured by the bromcresol green dye-binding method, the albumin concentration was 32.8 (SD 5.9) g/L. Similarly, the M-protein concentration in SPE was 49.5 (SD 12.3) g/L for the undiluted sera vs 61.8 (SD 15.1) g/L for the diluted sera, and the M-protein concentration in each diluted serum sample always exceeded that in the undiluted serum. Underestimation of M-protein limits the usefulness of M-protein measurement in evaluating the patient's response to therapy and for early detection of disease progression. SPE strips should be carefully inspected visually, and sera with M-protein band abnormalities should be diluted and re-assayed if SPE is to quantify concentrations of M-protein and albumin accurately.

Computer Calculation of Serum Protein Electrophoresis, Based on Peak Height Measurements

1970 ◽  
Vol 16 (9) ◽  
pp. 760-762 ◽  
Author(s):  
Sylvan M Sax ◽  
John J Moore
Keyword(s):  
Computer Program ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Computer Calculation ◽  
Peak Height ◽  
Protein Electrophoresis ◽  
Protein Fractions ◽  
Serum Protein Electrophoresis ◽  
Random Errors ◽  
Routine Application

Abstract A computer program has been written for off-line calculation of relative and absolute percentages of the five serum protein fractions usually seen on cellulose acetate electrophoretograms, with use of manually observed peak heights on densitometer scans. With myeloma-like peaks, both peak height and width at half-peak height must be measured. Routine application of the program saves technician time and decreases the number of large random errors.

Clinical usefulness of the molecular interpretation of the cellulose acetate serum protein electrophoresis

1980 ◽  
Vol 10 (1) ◽  
pp. 239-242
Author(s):  
Francesco Aguzzi ◽  
Concetta Petrini ◽  
Silvia Perolini ◽  
Gian Paolo Merlini ◽  
Marisa Maggi ◽  
...  
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Clinical Usefulness ◽  
Molecular Interpretation

scholarly journals Evaluation of serum protein electrophoresis by cellulose acetate membranes with different electro osmosis.

1991 ◽  
Vol 35 (1) ◽  
pp. 15-20
Author(s):  
Yoshiko Hirose ◽  
Katuichi Sasaki ◽  
Yasuko Yamagishi ◽  
Ikunosuke Sakurabayashi ◽  
Tadashi Kawai
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Cellulose Acetate Membranes ◽  
Electro Osmosis

Prediction of Immunoglobulins from Patterns of Cellulose Acetate Electrophoresis

1969 ◽  
Vol 15 (4) ◽  
pp. 271-281 ◽  
Author(s):  
M H Gault ◽  
S Hsieh ◽  
E Elzer
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Visual Analysis ◽  
Normal Values ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Cellulose Acetate Electrophoresis ◽  
Serum Immunoglobulins ◽  
Cellulose Acetate Membranes ◽  
Ig G

Abstract The concentrations of serum immunoglobulins (Ig) G, A, and M were correlated with specific features in the β-γ region of densitometer patterns of serum protein electrophoresis on cellulose acetate membranes; visual analysis of densitometer patterns permitted semiquantitative prediction of values for IgG and IgA. Important immunoglobulin abnormalities, particularly elevations of IgA, were seen in some cases even when normal values were obtained from analyses of electrophoresis by usual densitometer integrator technics; many such instances were detectable by inspection of the densitometer tracing.

Two new non-barbiturate buffers for electrophoresis of serum proteins on cellulose acetate membranes.

1980 ◽  
Vol 26 (8) ◽  
pp. 1221-1223 ◽  
Author(s):  
J Ambler ◽  
M Rodgers
Keyword(s):  
Sodium Chloride ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Drugs Of Abuse ◽  
Sodium Salicylate ◽  
Serum Proteins ◽  
Protein Electrophoresis ◽  
The Other ◽  
Serum Protein Electrophoresis ◽  
Cellulose Acetate Membranes

Abstract Two new non-barbiturate buffers have been formulated for serum protein electrophoresis on cellulose acetate membranes. Both buffers give five distrinct fractions and are suitable for all systems, but have been primarily designed to meet the needs of the Gelman "Sepratek" system. One buffer is a tris(hydroxymethyl)aminomethane (Tris)/hippurate formulation; the other is composed of Tris/N-[tris(hydroxymethyl)methyl]glycine (Tricine)/sodium chloride/sodium salicylate. Both buffers allow reliable quantitation of protein fractions, giving results that correlated well with those obtained from barbiturate separations. The major advantage of these buffers is that they do not contain drugs of abuse.

scholarly journals Diagnosing Paraproteinemic Keratopathy: A Case Report

2021 ◽  
pp. 337-343
Author(s):  
Eugenie Mok ◽  
Ka Wai Kam ◽  
Anthony J. Aldave ◽  
Alvin L. Young
Keyword(s):  
Optical Coherence Tomography ◽  
Genetic Testing ◽  
Serum Protein ◽  
Molecular Genetic ◽  
Anterior Segment ◽  
Protein Electrophoresis ◽  
Serum Protein Electrophoresis ◽  
Optical Coherence ◽  
Molecular Genetic Testing ◽  
Corneal Opacities

A 65-year-old man presented with bilateral, painless, progressive blurring of vision over 9 years. Slit-lamp examination revealed bilateral subepithelial corneal opacities in clusters located at the mid-periphery. Anterior segment optical coherence tomography, in vivo confocal microscopy (IVCM), serum protein electrophoresis, and molecular genetic testing were performed to evaluate the cause of corneal opacities. Anterior segment optical coherence tomography revealed a band-like, hyperreflective lesion in the Bowman layer and anterior stroma of both corneas. IVCM revealed hyperreflective deposits in the epithelium, anterior stroma, and endothelium. Serum protein electrophoresis identified the presence of paraproteins (immunoglobulin kappa), and molecular genetic testing revealed absence of mutations in the transforming growth factor beta-induced gene (<i>TGFBI</i>) and collagen type XVII alpha 1 gene (<i>COL17A1</i>). The ocular diagnosis of paraproteinemic keratopathy eventually led to a systemic diagnosis of monoclonal gammopathy of undetermined significance by our hematologist/oncologist. Paraproteinemic keratopathy is a rare differential diagnosis in patients with bilateral corneal opacities and therefore may be misdiagnosed as corneal dystrophy or neglected as scars. In patients with bilateral corneal opacities of unknown cause, serological examination, adjunct anterior segment imaging, and molecular genetic testing play a role in establishing the diagnosis.

Sign in / Sign up

Export Citation Format

Share Document