scholarly journals Evaluation of the new cellulose acetate membrane "SELECA-VSP" for serum protein fractionation using automated electrophoresis system "AES630".

2007 ◽  
Vol 51 (2) ◽  
pp. 129-133
Author(s):  
Kazuko Ohtake ◽  
Teruko Ohtake ◽  
Hiroko Fukada ◽  
Koji Horii ◽  
Mitsuru Murata
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Cellulose Acetate Membrane ◽  
Protein Fractionation ◽  
Electrophoresis System

Evaluation of a Cellulose-Acetate Electrophoresis System for Serum Protein Fractionation

1965 ◽  
Vol 11 (10) ◽  
pp. 937-942 ◽  
Author(s):  
Alex Kaplan ◽  
John Savory
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Blood Donors ◽  
Serum Proteins ◽  
Normal Values ◽  
Protein Fractionation ◽  
Cellulose Acetate Electrophoresis ◽  
Cellulose Acetate Membranes ◽  
Control Serum ◽  
Electrophoresis System

Abstract A rapid system for the quantitative fractionation of serum proteins by electrophoresis on cellulose-acetate membranes was evaluated and found to be quite precise. The reproducibility (coefficient of variation) of the routine fractionation of a control serum carried out 40 times during a 15-week period was 2.4% for albumin and 14.2, 6.0, 6.1, and 5.2%, respectively, for the α1-, α2-, β-, and γ-globulin fractions. Normal values are given for serum protein fractions (specimens from nonprofessional blood donors) obtained by cellulose acetate electrophoresis.

Evaluation of the Microzone System for Simultaneous Electrophoresis of 16 Sera

1971 ◽  
Vol 17 (1) ◽  
pp. 34-36 ◽  
Author(s):  
Benjamin Fingerhut ◽  
Antonia Ortiz
Keyword(s):  
Cellulose Acetate ◽  
Cellulose Acetate Membrane ◽  
Large Numbers ◽  
Sample Application ◽  
Electrophoresis System ◽  
Microzone Electrophoresis ◽  
Abnormal Results

Abstract We investigated the feasibility of using the "Microzone" electrophoresis system to produce 16 instead of eight electrophorograms on a single cellulose—acetate membrane by placing eight specimens at the left of center, rotating the cellulose—acetate membrane and supporting bridge 180° horizontally, and again applying eight samples to the left of center. The membrane, now with eight samples each on anodic and cathodic sides, is electrophoresed according to the usual recommended procedure. There was a statistically demonstrated association between sample application position and results (for 96 samples). This technique is nevertheless useful for determining albumin/globulin ratios and for screening large numbers of samples. Sera with borderline or abnormal results should be rerun by the usual technique.

THE CONSTRUCTION OF AN ULTRAFILTRATION UNIT TO MEASURE THE BINDING OF CERTAIN NITROGEN MUSTARDS TO SERUM PROTEIN

1962 ◽  
Vol 40 (1) ◽  
pp. 137-146 ◽  
Author(s):  
J. H. Linford ◽  
J. Legal ◽  
E. Zacharias
Keyword(s):  
Blood Serum ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Human Blood ◽  
Human Blood Serum ◽  
Cellulose Acetate Membrane ◽  
Nitrogen Mustards ◽  
Benzene Rings ◽  
High Degree

An ultrafiltration unit has been designed, and constructed to contain a 10-ml volume. The unit is leakproof, is self-contained, and can be operated inside a small refrigerator or oven. It has been used with "Visking" cellulose acetate membrane to separate the colloidal protein phase of human blood serum from the aqueous dispersing phase, and to study the distribution of certain nitrogen mustards between these phases. The results indicate that these drugs are adsorbed to the protein to a high degree and that the presence of the benzene rings in the nitrogen mustards is mainly responsible for this adsorption.

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