scholarly journals Improvement of the standard electrophoresis procedure for serum protein fractions. Using cellulose acetate membrane without the property of electroosmosis.

1992 ◽  
Vol 36 (4) ◽  
pp. 223-228
Author(s):  
Hiroko Cho ◽  
Kiyoko Shiba
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Fractions ◽  
Cellulose Acetate Membrane

Imprecision of quantification of serum protein fractions by electrophoresis on cellulose acetate.

1986 ◽  
Vol 32 (2) ◽  
pp. 356-357 ◽  
Author(s):  
S N Kahn ◽  
L P Strony
Keyword(s):  
High Resolution ◽  
Serum Albumin ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Protein Fractions ◽  
Visual Interpretation ◽  
Cellulose Acetate Electrophoresis ◽  
Trained Observer ◽  
Electrophoretic Patterns

Abstract We studied the precision of densitometric quantification of the protein zones resolved by cellulose acetate electrophoresis. Replicate analyses of patients' samples by a single technologist showed mean CVs ranging from 2.9% for serum albumin to 9.5% for alpha 1-globulin. There were marked differences in measurements obtained by replicate analysis of the same samples by two experienced technologists. We calculated what changes in fractional concentrations would be analytically significant and concluded that densitometry of cellulose acetate electrophoretograms can only be semi-quantitative. We suggest that visual interpretation of high-resolution electrophoretic patterns by a trained observer can replace densitometry in most cases.

Computer Calculation of Serum Protein Electrophoresis, Based on Peak Height Measurements

1970 ◽  
Vol 16 (9) ◽  
pp. 760-762 ◽  
Author(s):  
Sylvan M Sax ◽  
John J Moore
Keyword(s):  
Computer Program ◽  
Cellulose Acetate ◽  
Serum Protein ◽  
Computer Calculation ◽  
Peak Height ◽  
Protein Electrophoresis ◽  
Protein Fractions ◽  
Serum Protein Electrophoresis ◽  
Random Errors ◽  
Routine Application

Abstract A computer program has been written for off-line calculation of relative and absolute percentages of the five serum protein fractions usually seen on cellulose acetate electrophoretograms, with use of manually observed peak heights on densitometer scans. With myeloma-like peaks, both peak height and width at half-peak height must be measured. Routine application of the program saves technician time and decreases the number of large random errors.

Improved method for fractionating gamma-glutamyltransferase by electrophoresis on cellulose acetate.

1978 ◽  
Vol 24 (3) ◽  
pp. 502-504 ◽  
Author(s):  
A Burlina
Keyword(s):  
Cellulose Acetate ◽  
Serum Protein ◽  
Clinical Laboratory ◽  
Protein Fractions ◽  
Major Protein ◽  
Improved Method ◽  
Gamma Glutamyltransferase ◽  
Hepatic Diseases ◽  
Beta Globulin

Abstract I describe a method of fractionating gamma-glutamyltransferase on cellulose acetate. The tracing is obtained in parallel with that of serum protein, and the gamma-glutamyltransferase bands are characterized by correspondence with the major protein fractions. The overall pattern of the isoenzyme activity in normal sera is one of activity in the alpha1- and alpha2-globulin regions. In hepatic diseases four bands are usually present, but some more specific observations are possible, e.g., the presence of an intense beta-globulin band in occlusive icterus, intra- or extrahepatic, and a marked alpha2-globulin band in alcoholism. The potentialities of this technique as a diagnostic and prognostic aid together with its simplicity prompt me to recommend its use in the clinical laboratory.

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