scholarly journals Influence of temperature and salinity on microbial structure of marine anammox bacteria

2012 ◽  
Vol 66 (5) ◽  
pp. 958-964 ◽  
Author(s):  
Takanori Awata ◽  
Katsuichiro Tanabe ◽  
Tomonori Kindaichi ◽  
Noriatsu Ozaki ◽  
Akiyoshi Ohashi
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Fixed Bed ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Influence Of Temperature ◽  
Anammox Activity

Anaerobic ammonium oxidation (anammox) is a type of biological oxidation mediated by a group of Planctomycete-like bacteria. Members of the genus Candidatus Scalindua are mainly found in marine environments, but not exclusively. This group is cultured using different inoculums and conditions; however, its optimal growth conditions are not clear. Additionally, little information is known about the factors that influence the activity and the selection of a population of marine anammox bacteria. This study was conducted to investigate the influence of temperature and salinity on the marine anammox community. To accomplish this, an up-flow fixed-bed column reactor was operated, and quantitative fluorescence in situ hybridization (FISH) with probes specific to dominant marine anammox bacteria was conducted. Anammox activity was observed at 20 and 30 °C, but not at 10 °C. A nitrogen removal rate of 0.32 kg TN m–3 day–1 was obtained at 20 °C. These results suggest that temperature affects the activity (nitrogen removal rate) of anammox bacteria, while salinity does not affect the activity in the marine anammox biofilm.

scholarly journals Effects of biotin on promoting anammox bacterial activity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qinyu Li ◽  
Jinhui Chen ◽  
Guo-hua Liu ◽  
Xianglong Xu ◽  
Qian Zhang ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Slow Growth ◽  
Wastewater Treatment Plants ◽  
Removal Rate ◽  
Bacterial Biomass ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Short Term ◽  
Anammox Activity ◽  
Total Nitrogen Removal

AbstractAnaerobic ammonium oxidation (anammox) bacteria significantly improve the efficiency and reduce cost of nitrogen removal in wastewater treatment plants. However, their slow growth and vulnerable activity limit the application of anammox technology. In this paper, the enhancement of biotin on the nitrogen removal activity of anammox bacteria in short-term batch experiments was studied. We found that biotin played a significant role in promoting anammox activity within a biotin concentration range of 0.1–1.5 mg/L. At a biotin concentration of 1.0 mg/L, the total nitrogen removal rate (NRR) increased by 112%, extracellular polymeric substance (EPS) secretion and heme production significantly improved, and anammox bacterial biomass increased to maximum levels. Moreover, the predominant genus of anammox bacteria was Candidatus Brocadia.

scholarly journals Physiological characteristics of the anaerobic ammonium-oxidizing bacterium ‘Candidatus Brocadia sinica’

Microbiology ◽  
2011 ◽  
Vol 157 (6) ◽  
pp. 1706-1713 ◽  
Author(s):  
Mamoru Oshiki ◽  
Masaki Shimokawa ◽  
Naoki Fujii ◽  
Hisashi Satoh ◽  
Satoshi Okabe
Keyword(s):  
Growth Rate ◽  
Removal Rate ◽  
Nitrite Reduction ◽  
Physiological Characteristics ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Phylogenetic Affiliation ◽  
Anammox Activity ◽  
Rate Kinetics ◽  
Kinetics Of

The present study investigated the phylogenetic affiliation and physiological characteristics of bacteria responsible for anaerobic ammonium oxidization (anammox); these bacteria were enriched in an anammox reactor with a nitrogen removal rate of 26.0 kg N m−3 day−1. The anammox bacteria were identified as representing ‘Candidatus Brocadia sinica’ on the basis of phylogenetic analysis of rRNA operon sequences. Physiological characteristics examined were growth rate, kinetics of ammonium oxidation and nitrite reduction, temperature, pH and inhibition of anammox. The maximum specific growth rate (μmax) was 0.0041 h−1, corresponding to a doubling time of 7 days. The half-saturation constants (K s) for ammonium and nitrite of ‘Ca. B. sinica’ were 28±4 and 86±4 µM, respectively, higher than those of ‘Candidatus Brocadia anammoxidans’ and ‘Candidatus Kuenenia stuttgartiensis’. The temperature and pH ranges of anammox activity were 25–45 °C and pH 6.5–8.8, respectively. Anammox activity was inhibited in the presence of nitrite (50 % inhibition at 16 mM), ethanol (91 % at 1 mM) and methanol (86 % at 1 mM). Anammox activities were 80 and 70 % of baseline in the presence of 20 mM phosphorus and 3 % salinity, respectively. The yield of biomass and dissolved organic carbon production in the culture supernatant were 0.062 and 0.005 mol C (mol NH 4 + )−1, respectively. This study compared physiological differences between three anammox bacterial enrichment cultures to provide a better understanding of anammox niche specificity in natural and man-made ecosystems.

scholarly journals Coupled anaerobic ammonium oxidation and hydrogenotrophic denitrification for simultaneous NH4-N and NO3-N removal

2018 ◽  
Vol 79 (5) ◽  
pp. 975-984 ◽  
Author(s):  
Tatsuru Kamei ◽  
Rawintra Eamrat ◽  
Kenta Shinoda ◽  
Yasuhiro Tanaka ◽  
Futaba Kazama
Keyword(s):  
Nitrogen Removal ◽  
Inorganic Nitrogen ◽  
Nitrate Removal ◽  
Fixed Bed ◽  
Anaerobic Ammonium Oxidation ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
N Removal ◽  
Anammox Process

Abstract Nitrate removal during anaerobic ammonium oxidation (anammox) treatment is a concern for optimization of the anammox process. This study demonstrated the applicability and long-term stability of the coupled anammox and hydrogenotrophic denitrification (CAHD) process as an alternative method for nitrate removal. Laboratory-scale fixed bed anammox reactors (FBR) supplied with H2 to support denitrification were operated under two types of synthetic water. The FBRs showed simultaneous NH4-N and NO3-N removal, indicating that the CAHD process can support NO3-N removal during the anammox process. Intermittent H2 supply (e.g. 5 mL/min for a 1-L reactor, 14/6-min on/off cycle) helped maintain the CAHD process without deteriorating its performance under long-term operation and resulted in a nitrogen removal rate of 0.21 kg-N/m3/d and ammonium, nitrate, and dissolved inorganic nitrogen removal efficiencies of 73.4%, 80.4%, and 77%, respectively. The microbial community structure related to the CAHD process was not influenced by changes in influent water quality, and included the anammox bacteria ‘Candidatus Jettenia’ and a Sulfuritalea hydrogenivorans-like species as the dominant bacteria even after long-term reactor operation, suggesting that these bacteria are key to the CAHD process. These results indicate that the CAHD process is a promising method for enhancing the efficiency of anammox process.

Anaerobic ammonium oxidation of ammonium-rich waste streams in fixed-bed reactors

2004 ◽  
Vol 49 (11-12) ◽  
pp. 77-82 ◽  
Author(s):  
C. Fux ◽  
V. Marchesi ◽  
I. Brunner ◽  
H. Siegrist
Keyword(s):  
Removal Rate ◽  
Elimination Rate ◽  
Fixed Bed ◽  
Treatment Plant ◽  
Experimental Period ◽  
Anaerobic Ammonium Oxidation ◽  
Synthetic Wastewater ◽  
Ammonium Oxidation ◽  
Fixed Bed Reactors ◽  
Anammox Activity

The feasibility of anaerobic ammonium oxidation (Anammox) in fixed-bed reactors was evaluated on laboratory and pilot scales. Using synthetic wastewater, the specific nitrogen removal rate was increased from 0.05-0.1 kgNm-3reactord-1 to 0.35-0.38 kgNm-3reactord-1 within a year (T = 22-27°C) in all applications. However, the anammox activity was seriously and repeatedly inhibited at prolonged high nitrite concentrations (e.g. six days at 30-50 gNO2-Nm-3) and recovery was always a lengthy process. But even at a moderate nitrite concentration (11 ± 10 gNO2-Nm-3), the observed specific growth rate was only 0.018 d-1 at 26.4 ± 0.8°C, which corresponds to approximately 0.025 d-1 at 30°C (doubling time: 28 days). In a second experimental period for another 250 days, one of the laboratory reactors was fed with partially nitrified sludge liquors from a domestic wastewater treatment plant (WWTP). In this case, the specific elimination rate was as high as 3.5 kgNm-3reactord-1 at 26-27°C. Independently of the feed, the average nitrogen elimination rate lay between 80-85% in all applications. An appropriate hydraulic design is essential to prevent clogging and local nitrite inhibition in fixed-bed reactors.

scholarly journals Start-Up of Anammox SBR from Non-Specific Inoculum and Process Acceleration Methods by Hydrazine

Water ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 350
Author(s):  
Ivar Zekker ◽  
Oleg Artemchuk ◽  
Ergo Rikmann ◽  
Kelvin Ohimai ◽  
Gourav Dhar Bhowmick ◽  
...  
Keyword(s):  
Nitrogen Removal ◽  
Biological Nutrient Removal ◽  
Anaerobic Sludge ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Reject Water ◽  
Start Up ◽  
Anammox Activity ◽  
Optimum Salinity ◽  
Anammox Process

Biological nutrient removal from wastewater to reach acceptable levels is needed to protect water resources and avoid eutrophication. The start-up of an anaerobic ammonium oxidation (anammox) process from scratch was investigated in a 20 L sequence batch reactor (SBR) inoculated with a mixture of aerobic and anaerobic sludge at 30 ± 0.5 °C with a hydraulic retention time (HRT) of 2–3 days. The use of NH4Cl, NaNO2, and reject water as nitrogen sources created different salinity periods, in which the anammox process performance was assessed: low (<0.2 g of Cl−/L), high (18.2 g of Cl−/L), or optimum salinity (0.5–2 g of Cl−/L). Reject water feeding gave the optimum salinity, with an average nitrogen removal efficiency of 80%, and a TNRR of 0.08 kg N/m3/d being achieved after 193 days. The main aim was to show the effect of a hydrazine addition on the specific anammox activity (SAA) and denitrification activity in the start-up process to boost the autotrophic nitrogen removal from scratch. The effect of the anammox intermediate hydrazine addition was tested to assess its concentration effect (range of 2–12.5 mg of N2H4/L) on diminishing denitrifier activity and accelerating anammox activity at the same time. Heterotrophic denitrifiers’ activity was diminished by all hydrazine additions compared to the control; 5 mg of N2H4/L added enhanced SAA compared to the control, achieving an SAA of 0.72 (±0.01) mg N/g MLSS/h, while the test with 7.5 mg of N2H4/L reached the highest overall SAA of 0.98 (±0.09) mg N g/MLSS/h. The addition of trace amounts of hydrazine for 6 h was also able to enhance SAA after inhibition by organic carbon source sodium acetate addition at a high C/N ratio of 10/1. The start-up of anammox bacteria from the aerobic–anaerobic suspended biomass was successful, with hydrazine significantly accelerating anammox activity and decreasing denitrifier activity, making the method applicable for side-stream as well as mainstream treatment.

Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study

2010 ◽  
Vol 61 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Y. Kawagoshi ◽  
Y. Nakamura ◽  
H. Kawashima ◽  
K. Fujisaki ◽  
K. Furukawa ◽  
...  
Keyword(s):  
Waste Disposal ◽  
Nitrogen Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Enrichment Culture ◽  
Removal Rate ◽  
Nitrogen Loading ◽  
Disposal Site ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Waste Disposal Site

Anaerobic ammonium oxidation (anammox) is a novel nitrogen pathway catalyzed by anammox bacteria which are obligate anaerobic chemoautotrophs. In this study, enrichment culture of marine anammox bacteria (MAAOB) from the samples related to seawater was conducted. Simultaneous removal of ammonium and nitrite was confirmed in continuous culture inoculated with sediment of a sea-based waste disposal site within 50 days. However, no simultaneous nitrogen removal was observed in cultures inoculated with seawater-acclimated denitrifying sludge or with muddy sediment of tideland even during 200 days. Nitrogen removal rate of 0.13 kg/m3/day was achieved at nitrogen loading rate of 0.16 kg/m3/day after 320th days in the culture inoculated with the sediment of waste disposal site. The nitrogen removal ratio between ammonium nitrogen and nitrite nitrogen was 1:1.07. Denaturing gradient gel electrophoresis (DGGE) analysis indicated that an abundance of the bacteria close to MAAOB and coexistence of ammonium oxidizing bacteria and denitrifying bacteria in the culture.

Development of a super high-rate Anammox reactor and in situ analysis of biofilm structure and function

2007 ◽  
Vol 55 (8-9) ◽  
pp. 9-17 ◽  
Author(s):  
Ikuo Tsushima ◽  
Yuji Ogasawara ◽  
Masaki Shimokawa ◽  
Tomonori Kindaichi ◽  
Satoshi Okabe
Keyword(s):  
Spatial Distribution ◽  
Removal Rate ◽  
Dry Weight ◽  
High Rate ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Practical Applications ◽  
Macro Scale ◽  
Anammox Process

The anaerobic ammonium oxidation (Anammox) process is a new efficient and cost effective method of ammonium removal from wastewater. Under strictly anoxic condition, ammonium is directly oxidised with nitrite as electron acceptor to dinitrogen gas. However, it is extremely difficult to cultivate Anammox bacteria due to their low growth rate. This suggests that a rapid and efficient start-up of Anammox process is the key to practical applications. To screen appropriate seeding sludge with high Anammox potential, a real-time quantitative PCR assay with newly designed primers has been developed. Thereafter, the seeding sludge with high abundance of Anammox bacteria (1.7 × 108 copies/mg-dry weight) was selected and inoculated into an upflow anaerobic biofilters (UABs). The UABs were operated for more than 1 year and the highest nitrogen removal rate of 24.0 kg-N m−3 day−1 was attained. In addition, the ecophysiology of Anammox bacteria (spatial distribution and in situ activity) in biofilms was analysed by combining a full-cycle 16S rRNA approach and microelectrodes. The microelectrode measurement clearly revealed that a successive vertical zonation of the partial nitrification (NH4+ to NO2−), Anammox reaction and denitrification was developed in the biofilm in the UAB. This result agreed with the spatial distribution of corresponding bacterial populations in the biofilm. We linked the micro-scale information (i.e. single cell and/or biofilm levels) with the macro-scale information (i.e. the reactor level) to understand the details of Anammox reaction occurring in the UABs.

scholarly journals Nitrogen removal and functional bacteria distribution of ANAMMOX at ambient temperature

2016 ◽  
Vol 6 (4) ◽  
pp. 476-483 ◽  
Author(s):  
Taotao Zeng ◽  
Dong Li ◽  
Wei Liao ◽  
Wenxin Qiu ◽  
Jie Zhang
Keyword(s):  
Ambient Temperature ◽  
Nitrogen Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Removal Rate ◽  
Rhodopseudomonas Palustris ◽  
Middle Part ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Ambient Temperatures ◽  
Gradient Gel Electrophoresis

In this study, an up-flow anaerobic biofilter (AF) was operated to investigate the efficiency of anaerobic ammonium oxidation (ANAMMOX) in treating low strength ammonia (46.5 mg/L) at ambient temperatures (20.3–23.2 °C). Microbial compositions and functional populations of the upper (140–190 cm), middle (40–140 cm), and lower (0–40 cm) parts of the biofilter were monitored using scanning electron microscopy, denaturing gradient gel electrophoresis (DGGE), clone and sequence. The results show that stable biofilter performance was achieved with an average nitrogen removal rate of 2.26 kg/(m3·d) and a total nitrogen removal efficiency of 75.9%. Approximately 67% of the ammonia and nitrite disappeared in the middle part of the biofilter. The spherical bacteria, similar to ANAMMOX bacteria, dominated the middle part of the biofilter. There were eight bacterial DGGE bands; clone and sequence results showed that they included Oxalicibacterium sp., Ignavibacterium album, Bacterium rJ15, Candidatus Kuenenia stuttgartiensis, Hippea maritima, Thioprofundum lithotrophica, and Rhodopseudomonas palustris. The genus of ANAMMOX bacterium remaining at constant levels in different parts of the biofilter was identified as Candidatus Kuenenia stuttgartiensis. The AF bioreactor maintained high activity due to the ANAMMOX bacteria's ability to adapt to ambient temperature and low matrix influent conditions.

scholarly journals Nitrogen removal performance using anaerobic ammonium oxidation considering variable conditions

2021 ◽  
Vol 104 (3) ◽  
pp. 003685042110334
Author(s):  
Junmin Wang ◽  
Lei Fu
Keyword(s):  
Nitrogen Removal ◽  
High Efficiency ◽  
Removal Rate ◽  
Granular Sludge ◽  
Anaerobic Sludge ◽  
Anammox Bacteria ◽  
Stoichiometric Ratio ◽  
Ammonium Oxidation ◽  
Short Period ◽  
Anaerobic Sludge Blanket

The anaerobic nitrogen removal performance of anammox at 30°C, 25°C, and 16°C were studied by using the UASB (Up flow Anaerobic Sludge Blanket) reactor and the influent concentration of NH4+-N and NO2−-N were 16.9 and 20.6 mg L−1 respectively. Experimental results showed that high-efficiency anammox nitrogen removal could be achieved at 30°C, when hydraulic retention time (HRT) was 0.14 h, the nitrogen removal rate (NRR) was 5.73 kg N m−3 d−1. The anammox reactor operated stably for more than 80 days under the condition of 16°C–20°C, and the high NRR of 2.78 kg N m−3 d−1 was obtained. In this experiment, DO had little effect on the activity of anammox granular sludge, and the nitrogen removal performance could be quickly recovered in a short period of time after being affected by DO. Moreover, the stoichiometric ratio of NO2−-N and NH4+-N consumption (ΔNO2−-N/ΔNH4+-N) and the stoichiometric ratio of NO3−-N production and NH4+-N conversion (ΔNO3−-N/ΔNH4+-N) were 1.21 ± 0.11and 0.25 ± 0.06 respectively at 30°C, which were very close to the theoretical value, it indicated that anammox bacteria were the dominant bacteria at 30°C.

scholarly journals Impact of salinity on the performance and microbial community of anaerobic ammonia oxidation (Anammox) using 16S rRNA High-throughput Sequencing technology

2017 ◽  
Vol 19 (3) ◽  
pp. 377-388 ◽  
Keyword(s):  
16S Rrna ◽  
High Throughput ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Community Dynamics ◽  
Removal Rate ◽  
System Response ◽  
Anammox Bacteria ◽  
Term Operation ◽  
Anammox Activity

Salinity is a key environmental factor for the successful application of anammox technology in wastewater treatment, because it impacts the activity and the community structure of anammox bacteria. In this study, the changes in activity and population shifts of an anammox system response to the elevated salt stress (0, 5, 10, 20, 30 and 40 g NaCl/L) were studied. The results show that the anammox reactor performed effectively even at 30 g NaCl/L salinity after an appropriate acclimatization. The nitrogen removal rate maintained at 0.28 g N L-1d-1 with the nitrogen removal efficiency of 76%, though the high environmental salinity might inhibit the anammox growth in the long-term operation. 16S rRNA high-throughput sequencing results revealed that Ca. Brocadia, Ca. Jettenia and Ca. Kuenenia were the dominant anammox bacteria at all salinities. Ca. Brocadia and Ca. Jettenia were quite sensitive to salinity, and 5 g NaCl/L dosing could cause a sharp decline in their abundance. Nevertheless, these three anammox genus finally survived in the system with a steady specific anammox activity of 0.13 g N g VSS-1d-1. Specially, a novel cluster, Brocadiaceae_unclassified, which possibly belongs to anammox bacteria, became the dominant genus at the salinity over 20 g NaCl/L and likely contributed partially to the nitrogen removals. Our findings elucidated the inherent link between community dynamics and anammox system performance and stability under salty environment, and proved that anammox technologies can be an effective technology for treatment of saline ammonia-rich wastewater.

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