The behavior of estrogenic substances in the biological treatment process of sewage

2003 ◽  
Vol 47 (9) ◽  
pp. 109-116 ◽  
Author(s):  
K. Onda ◽  
Y. Nakamura ◽  
C. Takatoh ◽  
A. Miya ◽  
Y. Katsu
Keyword(s):  
Biological Treatment ◽  
Estrogenic Activity ◽  
Pilot Scale ◽  
Screening Assay ◽  
Treated Water ◽  
Operational Conditions ◽  
17Β Estradiol ◽  
One Year ◽  
Batch Treatment ◽  
And Behavior

A study was conducted for about one year on the fate and behavior of estrogens, namely 17β-estradiol (E2), estrone (E1), and estriol (E3) in an activated sludge process of a pilot scale plant supplied with domestic sewage. A simultaneous analytical method for these three substances using LC-MS/MS was developed and applied to sewage samples. The average removal of E2 was 94.7%, while that of E3 was 96.9%. In contrast, the average removal of E1 was relatively low at 69.2% with a maximum concentration of 55.4 ng/L detected in the treated water. The theoretical values of estrogenic activity calculated from the concentrations of each natural estrogen in treated water were found to correlate with the values of estrogenic activity measured by a yeast estrogen screening assay. The effect of E2 on estrogenic activity in influent was found to be high, while that of E1 in treated water was considerably higher. In batch treatment tests on E2, E2 turned into E1 immediately after being charged. After three hours of aeration, the values of both E1 and E2 were around threshold limits. It was determined from this that E1 and E2 were substances that could be degraded by biological treatment. As the removal of E2 was found to be sufficiently high at times, optimization of operational conditions based on E1 removal should be important for reducing estrogenic activity in treated water.

Detection and removal of estrogencity in membrane biological reactors

2006 ◽  
Vol 6 (6) ◽  
pp. 19-26 ◽  
Author(s):  
J.Y. Hu ◽  
X. Chen
Keyword(s):  
Estrogenic Activity ◽  
Solid Phase ◽  
Treatment Plant ◽  
Endocrine Disrupting Compounds ◽  
Pilot Scale ◽  
Membrane Bioreactors ◽  
Yeast Cells ◽  
Endocrine Disrupting ◽  
And Behavior ◽  
Fate And Behavior

Three pilot-scale submerged membrane bioreactors (MBRs) in a local wastewater treatment plant (K, M and Z) were studied with the objective to compare the performance of pre-denitrification MBR systems in eliminating the estrogenic activity of the effluent of primary clarifier. A total of 5 batches of samples, which included influent, effluent, supernatant and sludge from the respective aerobic and anoxic tanks were collected over the span. They were investigated by using the developed solid-phase extraction (SPE) protocol coupled with a modified yeast-based estrogen screen (YES) assay. From the results, it could be seen that M MBR demonstrated the best endocrine disrupting compounds (EDCs) removal efficiency. The fate and behavior of EDCs in MBR systems were fairly understood with estrogenic activity formation dominating in the anoxic tank and removal dominating in the aerobic tank. It is believed that the sorption of EDCs onto the sludge as well as biodegradation of EDCs might be the key mechanisms for the EDCs removal. The low response of YES when dealing with influent samples was mainly due to the inhibition and antagonist effects induced by the influent samples on yeast cells.

Estrogen and estrogen mimics contamination in water and the role of sewage treatment

2000 ◽  
Vol 42 (12) ◽  
pp. 173-179 ◽  
Author(s):  
S. Matsui ◽  
H. Takigami ◽  
T. Matsuda ◽  
N. Taniguchi ◽  
J. Adachi ◽  
...  
Keyword(s):  
Treatment Process ◽  
Estrogenic Activity ◽  
Sewage Treatment ◽  
Yeast Estrogen Screen ◽  
Scientific Debate ◽  
Treated Effluent ◽  
17Β Estradiol ◽  
And Behavior ◽  
Fate And Behavior

The yeast estrogen screen was applied to sewage treatment process waters to identify the presence of estrogenic activity and to investigate the fate and behavior of estrogenic substances through treatment. Hydrophobic fractions in the water phase were extracted and concentrated using C18 cartridges for the effective extraction of 17β-estradiol (E2) and other estrogen mimics. Clear dose-dependent elevation in the synthesis of β-galactosidase in the yeast screen was observed with all the samples tested, demonstrating that these samples were estrogenic. However, estrogenic activity tended to reduce during the treatment, especiallyiin the biological process. Quantification results of the yeast estrogen screen in terms of E2 equivalent were compared with actual E2 concentrations measured by an ELISA. E2 occupied 34% of the whole estrogenicity in the raw sewage, while almost 100% in the final effluent. The analyses of the sewage treatment process waters revealed that human estrogens are major causative substances in terms of estrogenic activity in sewage and its treated effluent. Although findings of possible correlation of environmental estrogens with the real impact on human health and the ecosystem are still the focus of scientific debate and investigation, proper management should be established in the sewage treatment process which receives various environmental contaminants.

Comparison of Fenton's oxidation and ozonation for removal of estrogens

2011 ◽  
Vol 63 (10) ◽  
pp. 2131-2137 ◽  
Author(s):  
Jana Nakrst ◽  
Mirjana Bistan ◽  
Tatjana Tišler ◽  
Jana Zagorc-Končan ◽  
Jan Derco ◽  
...  
Keyword(s):  
Estrogenic Activity ◽  
Complete Removal ◽  
Molar Ratio ◽  
Chemical Analyses ◽  
Screening Assay ◽  
Molar Ratios ◽  
Fenton’S Oxidation ◽  
17Β Estradiol ◽  
Fenton's Oxidation ◽  
By Products

This study compares efficiency of Fenton's oxidation and ozonation of 17β-estradiol (E2) and 17α-ethinylestradiol (EE2) as two possible processes for removal of estrogens from aqueous solutions. The effectiveness of Fenton's oxidative removal was studied at different ratios of reagents Fe2+:H2O2 (1:0.5; 1:10; 1:20; 1:33), where with some molar ratios up to 100% removal of E2 and EE2 was achieved in the first few minutes of reaction. The best molar ratio for E2 (17β-estradiol) removal was 1:33, while in the case of EE2 the most efficient one was 1:20 ratio. Ozonation was much faster, because complete removal of estrogens was achieved in 30 seconds (pH≈6), but the time of ozonation was extended up to 60 minutes trying to decompose formed by-products, expressing estrogenic activity, detected by YES (Yeast Estrogen Screening) assay. The obtained results showed that the removal efficiency of estrogens from waters should be assessed by a combination of chemical analyses and bioassay.

scholarly journals A New Anti-Estrogen Discovery Platform Identifies FDA-Approved Imidazole Anti-Fungal Drugs as Bioactive Compounds against ERα Expressing Breast Cancer Cells

2021 ◽  
Vol 22 (6) ◽  
pp. 2915
Author(s):  
Manuela Cipolletti ◽  
Stefania Bartoloni ◽  
Claudia Busonero ◽  
Martina Parente ◽  
Stefano Leone ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bioactive Compounds ◽  
Estrogenic Activity ◽  
Estrogen Receptor Α ◽  
Breast Cancers ◽  
Cellular Models ◽  
Cancer Models ◽  
17Β Estradiol ◽  
Approved Drugs ◽  
Anti Fungal

17β-estradiol (E2) exerts its physiological effects through the estrogen receptor α (i.e., ERα). The E2:ERα signaling allows the regulation of cell proliferation. Indeed, E2 sustains the progression of ERα positive (ERα+) breast cancers (BCs). The presence of ERα at the BC diagnosis drives their therapeutic treatment with the endocrine therapy (ET), which restrains BC progression. Nonetheless, many patients develop metastatic BCs (MBC) for which a treatment is not available. Consequently, the actual challenge is to complement the drugs available to fight ERα+ primary and MBC. Here we exploited a novel anti-estrogen discovery platform to identify new Food and Drug Administration (FDA)-approved drugs inhibiting E2:ERα signaling to cell proliferation in cellular models of primary and MBC cells. We report that the anti-fungal drugs clotrimazole (Clo) and fenticonazole (Fenti) induce ERα degradation and prevent ERα transcriptional signaling and proliferation in cells modeling primary and metastatic BC. The anti-proliferative effects of Clo and Fenti occur also in 3D cancer models (i.e., tumor spheroids) and in a synergic manner with the CDK4/CDK6 inhibitors palbociclib and abemaciclib. Therefore, Clo and Fenti behave as “anti-estrogens”-like drugs. Remarkably, the present “anti-estrogen” discovery platform represents a valuable method to rapidly identify bioactive compounds with anti-estrogenic activity.

scholarly journals Crosstalk between Existential Phenomenological Psychotherapy and Neurological Sciences in Mood and Anxiety Disorders

Biomedicines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 340
Author(s):  
Lehel Balogh ◽  
Masaru Tanaka ◽  
Nóra Török ◽  
László Vécsei ◽  
Shigeru Taguchi
Keyword(s):  
Anxiety Disorders ◽  
Biological Treatment ◽  
Sense Of Self ◽  
Phenomenological Approach ◽  
Neurological Damage ◽  
Mood And Anxiety Disorders ◽  
New Interpretation ◽  
And Behavior ◽  
The Impact ◽  
The Brain

Psychotherapy is a comprehensive biological treatment modifying complex underlying cognitive, emotional, behavioral, and regulatory responses in the brain, leading patients with mental illness to a new interpretation of the sense of self and others. Psychotherapy is an art of science integrated with psychology and/or philosophy. Neurological sciences study the neurological basis of cognition, memory, and behavior as well as the impact of neurological damage and disease on these functions, and their treatment. Both psychotherapy and neurological sciences deal with the brain; nevertheless, they continue to stay polarized. Existential phenomenological psychotherapy (EPP) has been in the forefront of meaning-centered counseling for almost a century. The phenomenological approach in psychotherapy originated in the works of Martin Heidegger, Ludwig Binswanger, Medard Boss, and Viktor Frankl, and it has been committed to accounting for the existential possibilities and limitations of one’s life. EPP provides philosophically rich interpretations and empowers counseling techniques to assist mentally suffering individuals by finding meaning and purpose to life. The approach has proven to be effective in treating mood and anxiety disorders. This narrative review article demonstrates the development of EPP, the therapeutic methodology, evidence-based accounts of its curative techniques, current understanding of mood and anxiety disorders in neurological sciences, and a possible converging path to translate and integrate meaning-centered psychotherapy and neuroscience, concluding that the EPP may potentially play a synergistic role with the currently prevailing medication-based approaches for the treatment of mood and anxiety disorders.

scholarly journals Pilot-scale study of vertical flow constructed wetland combined with trickling filter and ferric chloride coagulation: influence of irregular operational conditions

2015 ◽  
Vol 71 (7) ◽  
pp. 1088-1096 ◽  
Author(s):  
B. Kim ◽  
M. Gautier ◽  
G. Olvera Palma ◽  
P. Molle ◽  
P. Michel ◽  
...  
Keyword(s):  
Constructed Wetland ◽  
Phosphorus Removal ◽  
Water Saturation ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Saturation Level ◽  
Vertical Flow ◽  
Trickling Filter ◽  
Operational Conditions ◽  
Vertical Flow Constructed Wetland

The aim of this study was to characterize the efficiency of an intensified process of vertical flow constructed wetland having the following particularities: (i) biological pretreatment by trickling filter, (ii) FeCl3 injection for dissolved phosphorus removal and (iii) succession of different levels of redox conditions along the process line. A pilot-scale set-up designed to simulate a real-scale plant was constructed and operated using real wastewater. The influences of FeCl3 injection and water saturation level within the vertical flow constructed wetland stage on treatment performances were studied. Three different water saturation levels were compared by monitoring: suspended solids (SS), total phosphorus (TP), dissolved chemical oxygen demand (COD), ammonium, nitrate, phosphate, iron, and manganese. The results confirmed the good overall efficiency of the process and the contribution of the trickling filter pretreatment to COD removal and nitrification. The effects of water saturation level and FeCl3 injection on phosphorus removal were evaluated by analysis of the correlations between the variables. Under unsaturated conditions, good nitrification and no denitrification were observed. Under partly saturated conditions, both nitrification and denitrification were obtained, along with a good retention of SSs. Finally, under saturated conditions, the performance was decreased for almost all parameters.

Operational conditions for start-up and nitrate-feeding in an anoxic biotrickling filtration process at pilot scale

2016 ◽  
Vol 285 ◽  
pp. 83-91 ◽  
Author(s):  
Fernando Almenglo ◽  
Martín Ramírez ◽  
José Manuel Gómez ◽  
Domingo Cantero
Keyword(s):  
Pilot Scale ◽  
Filtration Process ◽  
Operational Conditions ◽  
Start Up

A New Sewage Treatment System with Fluidized Pellet Bed Separator

1993 ◽  
Vol 27 (11) ◽  
pp. 185-192 ◽  
Author(s):  
Tatsuhiko Suzuki ◽  
Norihito Tambo ◽  
Genzo Ozawa
Keyword(s):  
Molecular Weight ◽  
Biological Treatment ◽  
Membrane Filter ◽  
Sewage Treatment ◽  
Treatment System ◽  
Organic Substances ◽  
Treatment Rate ◽  
Treated Water ◽  
Biological Filter ◽  
Sewage Treatment System

A new sewage treatment system which is composed of a fluidized pellet bed separator, aerobic biological filter and micro–membrane filter was studied. In the newly proposed system, at the first stage, higher molecular weight organic substances and suspended matter are removed by way of chemical coagulation and flocculation. For the operation, a new fluidized pellet bed separator with high separation rate of 200-300 m/day is introduced to improve the treatment rate. A large percentage of organ ic substances are removed by the new coagulation/flocculation process. Hence, the total BOD load to be applied to the following biological treatment becomes very low. In addition to this, the organic substances in the effluent from the fluidized pellet bed separator are on lylower molecular weight substances which are easily biologically decomposed. Therefore easily decomposing and lower concentration pollutants enable use of a simple biological treatment p rocess such as aerobic biological filter with as short a detention time as 1 hour. Finally, effluent from the biological filter is treated by a micro-membrane filter. By the membrane process, suspended matte rs in the biologically treated water can be removed completely. Phosphate concentration of the treated water is very low, which is caused by aluminum coagulation in the first step and biological intake of the nutrient in the second step followed by complete sol id liquid separation in the third step.

Anaerobic co-digestion of winery waste and waste activated sludge: assessment of process feasibility

2013 ◽  
Vol 69 (2) ◽  
pp. 269-277 ◽  
Author(s):  
C. Da Ros ◽  
C. Cavinato ◽  
F. Cecchi ◽  
D. Bolzonella
Keyword(s):  
Activated Sludge ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Waste Activated Sludge ◽  
Organic Loading ◽  
Operational Conditions ◽  
Loading Rates ◽  
Thermophilic Conditions ◽  
Soluble Chemical Oxygen Demand

In this study the anaerobic co-digestion of wine lees together with waste activated sludge in mesophilic and thermophilic conditions was tested at pilot scale. Three organic loading rates (OLRs 2.8, 3.3 and 4.5 kgCOD/m3d) and hydraulic retention times (HRTs 21, 19 and 16 days) were applied to the reactors, in order to evaluate the best operational conditions for the maximization of the biogas yields. The addition of lee to sludge determined a higher biogas production: the best yield obtained was 0.40 Nm3biogas/kgCODfed. Because of the high presence of soluble chemical oxygen demand (COD) and polyphenols in wine lees, the best results in terms of yields and process stability were obtained when applying the lowest of the three organic loading rates tested together with mesophilic conditions.

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