Effect of Genotypes, Sucrose Concentrations and Their Interaction on Anther Culture Response on Summer Squash

2016 ◽  
Vol 7 (4) ◽  
pp. 113-120
Author(s):  
Rehab Habiba
1987 ◽  
Vol 74 (3) ◽  
pp. 334-338 ◽  
Author(s):  
W. R. Deaton ◽  
S. G. Metz ◽  
T. A. Armstrong ◽  
P. N. Mascia

2021 ◽  
Vol 55 (6) ◽  
pp. 590-597
Author(s):  
Th. B. Lazaridou ◽  
A. G. Mavromatis ◽  
I. N. Xynias

1993 ◽  
Vol 41 (2) ◽  
pp. 227 ◽  
Author(s):  
SJ Logue ◽  
LC Giles ◽  
DHB Sparrow

A screening of several Australian barley cultivars of commercial interest has identified a number of genotypes that respond well to anther culture, with average levels of green plant regeneration between 23 and 134 plants/100 anthers cultured. Donor plant growth conditions have a large impact on anther culture response and, although optimal conditions for specific genotypes could possibly be identified, it is likely to be more effective for the production of large numbers of doubled haploids to settle for a broadly acceptable environment. Recent advances in methodology and the identification of responsive genotypes makes anther culture a feasible procedure for Australian barley breeding programs.


1995 ◽  
Vol 35 (3) ◽  
pp. 395 ◽  
Author(s):  
DC Ling ◽  
DJ Luckett ◽  
NL Darvey ◽  
XL Ding

Anther culture response in a diverse range of Australian wheat-breeding genotypes was tested and expressed as a 'culturability index' based on the actual costs of the various laboratory procedures. When the delay from harvest to plating was up to 12 h, the use of low dose gamma irradiation did not increase the anther culture response in this array of genotypes. This is in contrast to previous findings for which spike harvesting, irradiation, and plating was completed within 3 h and the irradiation treatment increased the anther culture response. Internal contamination of immature spike explants with Fusarium poae caused significant loss of cultures and may be a persistent problem for cereal anther culture. For the genotypes producing green regenerants, costs ranged from $A1.40 to $85/green regenerant.


Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 152-157 ◽  
Author(s):  
W. Powell

The genetics of barley microspore development in culture was examined by means of diallel analysis. The frequency of microspore derived green and albino plant production was shown to be under genetic control. This genotypic limitation to microspore development will limit the application of anther culture techniques to barley breeding programmes. However, significant additive genetic effects were detected for the characters measured and indicate that the frequency of green plant regeneration may be improved by the hybridization of suitable parents. Significant reciprocal differences were also detected and indicate that the direction of the cross is important in determining microspore development. An embryogenic route to green plantlet formation was observed in a number of genotypes in the diallel experiment. The implications of these findings for barley improvement and genetics are discussed.Key words: doubled haploids, barley, anther culture, microspore, embryoid.


Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 968-975 ◽  
Author(s):  
V. H. Beaumont ◽  
T. R. Rocheford ◽  
J. M. Widholm

In order to map the genes conditioning the induction of embryos during our anther culture process, we evaluated F2 plants from three different crosses for their anther culture ability and also performed RFLP analysis on these plants. The results showed that six chromosomal regions appear to be associated with the ability to induce embryo-like structures from maize microspores. These regions are located on chromosomes 1 (two regions), 3, 5, 7, and 8. Some of these chromosomes are identical to those found in previous studies and we have localized the regions more precisely. Notably, all chromosome regions identified, except one, are near viviparous mutant loci. Since the viviparous mutations are known to involve the plant hormone abscisic acid (ABA), these results suggest that ABA or its antagonist, gibberellic acid (GA3), might somehow be related to anther culture ability. We also propose some combinations of probes to screen for anther culture ability in the three genotypes studied.Key words: restriction fragment length polymorphism, anther culture, gene mapping, Zea mays L.


2007 ◽  
Vol 32 (4) ◽  
pp. 2499-2509
Author(s):  
M. Abd El-Maksoud ◽  
A. Abd El-Hadi ◽  
A. Draz ◽  
W. El-Gamal

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