scholarly journals GENETICAL ANALYSIS OF ANTHER CULTURE RESPONSE IN RICE UNDER DROUGHT STRESS

2007 ◽  
Vol 32 (4) ◽  
pp. 2499-2509
Author(s):  
M. Abd El-Maksoud ◽  
A. Abd El-Hadi ◽  
A. Draz ◽  
W. El-Gamal
Keyword(s):  
Drought Stress ◽  
Anther Culture ◽  
Genetical Analysis ◽  
Anther Culture Response

Genetic analysis of the anther-culture response of three spring wheat crosses

1987 ◽  
Vol 74 (3) ◽  
pp. 334-338 ◽  
Author(s):  
W. R. Deaton ◽  
S. G. Metz ◽  
T. A. Armstrong ◽  
P. N. Mascia
Keyword(s):  
Genetic Analysis ◽  
Anther Culture ◽  
Spring Wheat ◽  
Anther Culture Response

Effect of Culture Medium and Mannitol Pre-Treatment on Durum Wheat Anther Culture Response

2021 ◽  
Vol 55 (6) ◽  
pp. 590-597
Author(s):  
Th. B. Lazaridou ◽  
A. G. Mavromatis ◽  
I. N. Xynias
Keyword(s):  
Durum Wheat ◽  
Anther Culture ◽  
Culture Medium ◽  
Pre Treatment ◽  
Anther Culture Response

Genotype and Environment Strongly Influence Barley Anther Culture Response Using Australian Genotypes

1993 ◽  
Vol 41 (2) ◽  
pp. 227 ◽  
Author(s):  
SJ Logue ◽  
LC Giles ◽  
DHB Sparrow
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Growth Conditions ◽  
Green Plant ◽  
Optimal Conditions ◽  
Donor Plant ◽  
Breeding Programs ◽  
Barley Cultivars ◽  
Large Numbers ◽  
Anther Culture Response

A screening of several Australian barley cultivars of commercial interest has identified a number of genotypes that respond well to anther culture, with average levels of green plant regeneration between 23 and 134 plants/100 anthers cultured. Donor plant growth conditions have a large impact on anther culture response and, although optimal conditions for specific genotypes could possibly be identified, it is likely to be more effective for the production of large numbers of doubled haploids to settle for a broadly acceptable environment. Recent advances in methodology and the identification of responsive genotypes makes anther culture a feasible procedure for Australian barley breeding programs.

A cost-based index of anther culture response in diverse wheat-breeding germplasm

1995 ◽  
Vol 35 (3) ◽  
pp. 395 ◽  
Author(s):  
DC Ling ◽  
DJ Luckett ◽  
NL Darvey ◽  
XL Ding
Keyword(s):  
Anther Culture ◽  
Low Dose ◽  
Wheat Breeding ◽  
Significant Loss ◽  
Diverse Range ◽  
Persistent Problem ◽  
Irradiation Treatment ◽  
Australian Wheat ◽  
Laboratory Procedures ◽  
Anther Culture Response

Anther culture response in a diverse range of Australian wheat-breeding genotypes was tested and expressed as a 'culturability index' based on the actual costs of the various laboratory procedures. When the delay from harvest to plating was up to 12 h, the use of low dose gamma irradiation did not increase the anther culture response in this array of genotypes. This is in contrast to previous findings for which spike harvesting, irradiation, and plating was completed within 3 h and the irradiation treatment increased the anther culture response. Internal contamination of immature spike explants with Fusarium poae caused significant loss of cultures and may be a persistent problem for cereal anther culture. For the genotypes producing green regenerants, costs ranged from $A1.40 to $85/green regenerant.

Diallel analysis of barley anther culture response

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 152-157 ◽  
Author(s):  
W. Powell
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Diallel Analysis ◽  
Green Plant ◽  
Plant Production ◽  
Microspore Development ◽  
Culture Techniques ◽  
Plantlet Formation ◽  
Breeding Programmes ◽  
Anther Culture Response

The genetics of barley microspore development in culture was examined by means of diallel analysis. The frequency of microspore derived green and albino plant production was shown to be under genetic control. This genotypic limitation to microspore development will limit the application of anther culture techniques to barley breeding programmes. However, significant additive genetic effects were detected for the characters measured and indicate that the frequency of green plant regeneration may be improved by the hybridization of suitable parents. Significant reciprocal differences were also detected and indicate that the direction of the cross is important in determining microspore development. An embryogenic route to green plantlet formation was observed in a number of genotypes in the diallel experiment. The implications of these findings for barley improvement and genetics are discussed.Key words: doubled haploids, barley, anther culture, microspore, embryoid.

Mapping the anther culture response genes in maize (Zea mays L.)

Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 968-975 ◽  
Author(s):  
V. H. Beaumont ◽  
T. R. Rocheford ◽  
J. M. Widholm
Keyword(s):  
Zea Mays ◽  
Anther Culture ◽  
Plant Hormone ◽  
Zea Mays L ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Culture Process ◽  
Anther Culture Response ◽  
Chromosome Regions ◽  
Restriction Fragment Length

In order to map the genes conditioning the induction of embryos during our anther culture process, we evaluated F2 plants from three different crosses for their anther culture ability and also performed RFLP analysis on these plants. The results showed that six chromosomal regions appear to be associated with the ability to induce embryo-like structures from maize microspores. These regions are located on chromosomes 1 (two regions), 3, 5, 7, and 8. Some of these chromosomes are identical to those found in previous studies and we have localized the regions more precisely. Notably, all chromosome regions identified, except one, are near viviparous mutant loci. Since the viviparous mutations are known to involve the plant hormone abscisic acid (ABA), these results suggest that ABA or its antagonist, gibberellic acid (GA3), might somehow be related to anther culture ability. We also propose some combinations of probes to screen for anther culture ability in the three genotypes studied.Key words: restriction fragment length polymorphism, anther culture, gene mapping, Zea mays L.

scholarly journals Gametic Embryogenic Response in Wild Diploid Solanum Species and its Implications for Genome Sequencing Projects and Breeding

2016 ◽  
Vol 26 (2) ◽  
pp. 159-173
Author(s):  
A Castillo ◽  
P Gaiero ◽  
B López Carro ◽  
F Vilaró
Keyword(s):  
Anther Culture ◽  
Culture Media ◽  
Solanum Species ◽  
Chromosome Counts ◽  
Solanum Commersonii ◽  
Potato Germplasm ◽  
Anther Culture Response ◽  
Anther Cultures ◽  
Embryogenic Response ◽  
Haploid Plants

The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding.Plant Tissue Cult. & Biotech. 26(2): 159-173, 2016 (December)

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