scholarly journals Assessment of Healing in Calvarial Bone Defect by Allogenic Demineralized Bone Matrix and Adipose Derived Stem Cells

2018 ◽  
Vol 42 (2) ◽  
pp. 353-362
Author(s):  
Mohamed Badawi ◽  
Saad Elfayomy ◽  
Basim Zaki ◽  
Abdelrahman Sayed ◽  
Fatma Abuzahra
Keyword(s):  
Stem Cells ◽  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Adipose Derived Stem Cells ◽  
Calvarial Bone ◽  
Calvarial Bone Defect ◽  
Demineralized Bone

scholarly journals Simultaneous Treatment of Photobiomodulation and Demineralized Bone Matrix With Adipose-Derived Stem Cells Improve Bone Healing in an osteoporotic bone defect

2021 ◽  
Vol 12 (1) ◽  
pp. e41-e41
Author(s):  
Rouhallah Gazor ◽  
Mehrdad Asgari ◽  
Mohammad-Amin Abdollajhifar ◽  
Pejman Kiani ◽  
Fatemeh Zare ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Healing ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Adipose Derived Stem Cells ◽  
Simultaneous Treatment ◽  
Whitney Test ◽  
Mann Whitney Test ◽  
Ovx Rats ◽  
Demineralized Bone

Introduction: The ability of simultaneous treatment of critical-sized femoral defects (CSFDs) with photobiomodulation (PBM) and demineralized bone matrix (DBM) with or without seeded adipose-derived stem cells (ASCs) to induce bone reconstruction in ovariectomized induced osteoporotic (OVX) rats was investigated. Methods: The OVX rats with CSFD were arbitrarily separated into 6 groups: control, scaffold (S, DBM), S + PBM, S + alendronate (ALN), S + ASCs, and S + PBM + ASCs. Each group was assessed by cone beam computed tomography (CBCT) and histological examinations. Results: In the fourth week, CBCT and histological analyses revealed that the largest volume of new bone formed in the S + PBM and S + PBM + ASC groups. The S + PBM treatment relative to the S and S + ALN treatments remarkably reduced the CSFD (Mann-Whitney test, P = 0.009 and P = 0.01). Furthermore, S + PBM + ASCs treatment compared to the S and S + ALN treatments significantly decreased CSFD (Mann Whitney test, P = 0.01). In the eighth week, CBCT analysis showed that extremely enhanced bone regeneration occurred in the CSFD of the S + PBM group. Moreover, the CSFD in the S + PBM group was substantially smaller than S, S + ALN and S + ASCs groups (Mann Whitney test, P = 0.01, P = 0.02 and P = 0.009). Histological observations showed more new bone formation in the treated CSFD of S + PBM + ASCs and S + PBM groups. Conclusion: The PBM plus DBM with or without ASCs significantly enhanced bone healing in the CSFD in OVX rats compared to control, DBM alone, and ALN plus DBM groups. The PBM plus DBM with or without ASCs significantly decreased the CSFD area compared to either the solo DBM or ALN plus DBM treatments.

scholarly journals Histopathological and Radiographical Evaluation of Caprine Demineralized Bone Matrix in a Critical Ulnar Defect in a Rabbit Model

2021 ◽  
Author(s):  
Olawale Alimi Alimi ◽  
Adamu Abdul Abuabakar ◽  
Abubakar Sadiq Yakubu ◽  
Sani Abdullahi Shehu ◽  
Salman Zubairu Abdulkadir
Keyword(s):  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rabbit Model ◽  
Negative Control ◽  
Autogenous Bone ◽  
Level Of Evidence ◽  
Defect Sites ◽  
Significant Difference ◽  
Demineralized Bone

Abstract Background: Caprine species satisfy the conditions of an ideal donor animal when compared to bovine species that has been extensively studied and commercialized for bone xenograft. Histopathological and radiological evaluations of caprine demineralized bone matrix (CDBM) were therefore carried out for fracture healing properties for its possible use in bone grafting procedures. Materials and Methods: Twenty-four rabbits were used for this study and were divided randomly into three groups of eight (n=8) rabbits each. Critical bone defect was created on the ulnar diaphysis under xylazine-ketamine anaesthesia for autogenous bone graft (ABG) group, CDBM group and the last group was left unfilled as negative control (NC). Immediate post-grafting radiograph was taken and repeated on days 14, 28, 42 and 56 to monitor the evidence of radiographic healing. The animals were euthanized on day 56 and defect sites were harvested for histopathology. Results: There was a progressive evidence of radiographic healing and bone formation in all the groups with significance difference (P=0.0064). When compared with ABG, NC differ significantly (P<0.0001) whereas the CDBM did not differ significantly (P=0.6765). The histopathology sections of ABG and CDBM showed normal bone tissue while the NC section was predominated by fibrous connective tissue. There was therefore an overall significant difference (P=0.0001) in which CDBM did not differ from ABG (P=0.2946) while NC did (P=0.0005). Conclusion: The ABG and CDBM groups showed a similar healing effect in the critical bone defect. Therefore, CDBM could be used as an effective alternative to ABG in orthopaedics to circumvent the limitations and complications associated with it. Level of Evidence: Not applicable.

Repair of Calvarial Bone Defect Using Jarid1a-Knockdown Bone Mesenchymal Stem Cells in Rats

2018 ◽  
Vol 24 (9-10) ◽  
pp. 711-718 ◽  
Author(s):  
Yuan Deng ◽  
Tao Guo ◽  
Jipeng Li ◽  
Li Guo ◽  
Ping Gu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Calvarial Bone ◽  
Bone Mesenchymal Stem Cells ◽  
Calvarial Bone Defect

scholarly journals Cell-Free Demineralized Bone Matrix for Mesenchymal Stem Cells Survival and Colonization

Materials ◽  
2019 ◽  
Vol 12 (9) ◽  
pp. 1360 ◽  
Author(s):  
Monica Mattioli-Belmonte ◽  
Francesca Montemurro ◽  
Caterina Licini ◽  
Iolanda Iezzi ◽  
Manuela Dicarlo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Morphological Observation ◽  
Type I ◽  
Demineralized Bone

Decellularized bone matrix is receiving much attention as biological scaffolds and implantable biomaterials for bone tissue regeneration. Here, we evaluated the efficacy of a cell-free demineralized bone matrix on mesenchymal stem cells (MSCs) survival and differentiation in vitro. The seeding of human umbilical cord-derived MSCs (hUC-SCs) on decellularized bone matrices up to 14 days was exploited, assessing their capability of scaffold colonization and evaluating gene expression of bone markers. Light and Scanning Electron Microscopies were used. The obtained cell-free decalcified structures showed elastic moduli attributable to both topology and biochemical composition. Morphological observation evidenced an almost complete colonization of the scaffolds after 14 days of culture. Moreover, in hUC-SCs cultured on decalcified scaffolds, without the addition of any osteoinductive media, there was an upregulation of Collagen Type I (COL1) and osteonectin (ON) gene expression, especially on day 14. Modifications in the expression of genes engaged in stemness were also detected. In conclusion, the proposed decellularized bone matrix can induce the in vitro hUC-SCs differentiation and has the potential to be tested for in in vivo tissue regeneration.

Characterization of Bone Defect Repair in Young and Aged Rat Femur Induced by Xenogenic Demineralized Bone Matrix

2002 ◽  
Vol 73 (9) ◽  
pp. 1003-1009 ◽  
Author(s):  
Paola Torricelli ◽  
Milena Fini ◽  
Gianluca Giavaresi ◽  
Lia Rimondini ◽  
Roberto Giardino
Keyword(s):  
Bone Defect ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Rat Femur ◽  
Aged Rat ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Demineralized Bone

Demineralized bone matrix-based microcarrier scaffold favors vascularized large bone regeneration in vivo in a rat model

2018 ◽  
Vol 33 (2) ◽  
pp. 182-195 ◽  
Author(s):  
Qiannan Li ◽  
Wenjie Zhang ◽  
Guangdong Zhou ◽  
Yilin Cao ◽  
Wei Liu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Stem Cells ◽  
Bone Marrow ◽  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Matrix Group ◽  
Micro Computed Tomography ◽  
Demineralized Bone

Insufficient neo-vascularization of in vivo implanted cell-seeded scaffold remains a major bottleneck for clinical translation of engineered bone formation. Demineralized bone matrix is an ideal bone scaffold for bone engineering due to its structural and biochemical components similar to those of native bone. We hypothesized that the microcarrier form of demineralized bone matrix favors ingrowth of vessels and bone regeneration upon in vivo implantation. In this study, a rat model of femoral vessel pedicle-based bone engineering was employed by filling the demineralized bone matrix scaffolds inside a silicone chamber that surrounded the vessel pedicles, and to compare the efficiency of vascularized bone regeneration between microcarrier demineralized bone matrix and block demineralized bone matrix. The results showed that bone marrow stem cells better adhered to microcarrier demineralized bone matrix and produced more extracellular matrices during in vitro culture. After in vivo implantation, microcarrier demineralized bone matrix seeded with bone marrow stem cells formed relatively more bone tissue than block demineralized bone matrix counterpart at three months upon histological examination. Furthermore, micro-computed tomography three-dimensional reconstruction showed that microcarrier demineralized bone matrix group regenerate significantly better and more bone tissues than block demineralized bone matrix both qualitatively and quantitatively (p < 0.05). Moreover, micro-computed tomography reconstructed angiographic images also demonstrated significantly enhanced tissue vascularization in microcarrier demineralized bone matrix group than in block demineralized bone matrix group both qualitatively and quantitatively (p < 0.05). Anti-CD31 immunohistochemical staining of (micro-) vessels and semi-quantitative analysis also evidenced enhanced vascularization of regenerated bone in microcarrier demineralized bone matrix group than in block demineralized bone matrix group (p < 0.05). In conclusion, the microcarrier form of demineralized bone matrix is an ideal bone regenerative scaffold due to its advantages of osteoinductivity and vascular induction, two essentials for in vivo bone regeneration.

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