In Vivo Evaluation of Mixtures of Uncultured Freshly Isolated Adipose-Derived Stem Cells and Demineralized Bone Matrix for Bone Regeneration in a Rat Critically Sized Calvarial Defect Model

2011 ◽  
Vol 20 (2) ◽  
pp. 233-242 ◽  
Author(s):  
Seung Chul Rhee ◽  
Yi-hwa Ji ◽  
Nareg A. Gharibjanian ◽  
Eun Sang Dhong ◽  
Seung Ha Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Adipose Derived Stem Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
In Vivo Evaluation ◽  
Demineralized Bone

Demineralized bone matrix-based microcarrier scaffold favors vascularized large bone regeneration in vivo in a rat model

2018 ◽  
Vol 33 (2) ◽  
pp. 182-195 ◽  
Author(s):  
Qiannan Li ◽  
Wenjie Zhang ◽  
Guangdong Zhou ◽  
Yilin Cao ◽  
Wei Liu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Stem Cells ◽  
Bone Marrow ◽  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Matrix Group ◽  
Micro Computed Tomography ◽  
Demineralized Bone

Insufficient neo-vascularization of in vivo implanted cell-seeded scaffold remains a major bottleneck for clinical translation of engineered bone formation. Demineralized bone matrix is an ideal bone scaffold for bone engineering due to its structural and biochemical components similar to those of native bone. We hypothesized that the microcarrier form of demineralized bone matrix favors ingrowth of vessels and bone regeneration upon in vivo implantation. In this study, a rat model of femoral vessel pedicle-based bone engineering was employed by filling the demineralized bone matrix scaffolds inside a silicone chamber that surrounded the vessel pedicles, and to compare the efficiency of vascularized bone regeneration between microcarrier demineralized bone matrix and block demineralized bone matrix. The results showed that bone marrow stem cells better adhered to microcarrier demineralized bone matrix and produced more extracellular matrices during in vitro culture. After in vivo implantation, microcarrier demineralized bone matrix seeded with bone marrow stem cells formed relatively more bone tissue than block demineralized bone matrix counterpart at three months upon histological examination. Furthermore, micro-computed tomography three-dimensional reconstruction showed that microcarrier demineralized bone matrix group regenerate significantly better and more bone tissues than block demineralized bone matrix both qualitatively and quantitatively (p < 0.05). Moreover, micro-computed tomography reconstructed angiographic images also demonstrated significantly enhanced tissue vascularization in microcarrier demineralized bone matrix group than in block demineralized bone matrix group both qualitatively and quantitatively (p < 0.05). Anti-CD31 immunohistochemical staining of (micro-) vessels and semi-quantitative analysis also evidenced enhanced vascularization of regenerated bone in microcarrier demineralized bone matrix group than in block demineralized bone matrix group (p < 0.05). In conclusion, the microcarrier form of demineralized bone matrix is an ideal bone regenerative scaffold due to its advantages of osteoinductivity and vascular induction, two essentials for in vivo bone regeneration.

The Effect of Carrier Type on Bone Regeneration of Demineralized Bone Matrix In Vivo

2013 ◽  
Vol 24 (6) ◽  
pp. 2135-2140 ◽  
Author(s):  
Shima Tavakol ◽  
Ahad Khoshzaban ◽  
Mahmoud Azami ◽  
Iraj Ragerdi Kashani ◽  
Hani Tavakol ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Demineralized Bone

In Vivo Evaluation of Demineralized Bone Matrix as a Bone Graft Substitute for Posterior Spinal Fusion

Spine ◽  
1995 ◽  
Vol 20 (8) ◽  
pp. 877-886 ◽  
Author(s):  
Stephen D. Cook ◽  
Jeanette E. Dalton ◽  
Ann B. Prewett ◽  
Thomas S. Whitecloud
Keyword(s):  
Bone Graft ◽  
Spinal Fusion ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Posterior Spinal Fusion ◽  
Bone Graft Substitute ◽  
In Vivo Evaluation ◽  
Demineralized Bone

In vitro and in vivo evaluation of xenogeneic bone putty with the carrier of hydrogel derived from demineralized bone matrix

2018 ◽  
Vol 19 (4) ◽  
pp. 591-601 ◽  
Author(s):  
Naili Zhang ◽  
Lina Ma ◽  
Xiaowei Liu ◽  
Xiaorui Jiang ◽  
Zhenhai Yu ◽  
...  
Keyword(s):  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
In Vivo Evaluation ◽  
Demineralized Bone ◽  
Bone Putty

Demineralized Bone Matrix Promotes Bone Regeneration In Vivo: A Canine Study

2018 ◽  
Vol 8 (5) ◽  
pp. 716-722
Author(s):  
Guolin Liu ◽  
Lingxiao Wang ◽  
Lei Hu ◽  
Changying Liu ◽  
Luyuan Jin ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Canine Study ◽  
Demineralized Bone
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