scholarly journals Farnesoid X receptor regulates the growth of renal adenocarcinoma cells without affecting that of a normal renal cell-derived cell line

2017 ◽  
Vol 42 (3) ◽  
pp. 259-265 ◽  
Author(s):  
Tomofumi Fujino ◽  
Ryosuke Sakamaki ◽  
Haruka Ito ◽  
Yumiko Furusato ◽  
Nami Sakamoto ◽  
...  
Keyword(s):  
Cell Line ◽  
Renal Cell ◽  
Farnesoid X Receptor ◽  
Renal Adenocarcinoma ◽  
Adenocarcinoma Cells

scholarly journals Metabolomic Analysis to Elucidate Mechanisms of Sunitinib Resistance in Renal Cell Carcinoma

Metabolites ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 1
Author(s):  
Tomonori Sato ◽  
Yoshihide Kawasaki ◽  
Masamitsu Maekawa ◽  
Shinya Takasaki ◽  
Kento Morozumi ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Energy Metabolism ◽  
Cell Carcinoma ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Progression ◽  
Renal Cell ◽  
Treatment Resistance ◽  
Tca Cycle ◽  
Glutamine Uptake

Metabolomics analysis possibly identifies new therapeutic targets in treatment resistance by measuring changes in metabolites accompanying cancer progression. We previously conducted a global metabolomics (G-Met) study of renal cell carcinoma (RCC) and identified metabolites that may be involved in sunitinib resistance in RCC. Here, we aimed to elucidate possible mechanisms of sunitinib resistance in RCC through intracellular metabolites. We established sunitinib-resistant and control RCC cell lines from tumor tissues of RCC cell (786-O)-injected mice. We also quantified characteristic metabolites identified in our G-Met study to compare intracellular metabolism between the two cell lines using liquid chromatography-mass spectrometry. The established sunitinib-resistant RCC cell line demonstrated significantly desuppressed protein kinase B (Akt) and mesenchymal-to-epithelial transition (MET) phosphorylation compared with the control RCC cell line under sunitinib exposure. Among identified metabolites, glutamine, glutamic acid, and α-KG (involved in glutamine uptake into the tricarboxylic acid (TCA) cycle for energy metabolism); fructose 6-phosphate, D-sedoheptulose 7-phosphate, and glucose 1-phosphate (involved in increased glycolysis and its intermediate metabolites); and glutathione and myoinositol (antioxidant effects) were significantly increased in the sunitinib-resistant RCC cell line. Particularly, glutamine transporter (SLC1A5) expression was significantly increased in sunitinib-resistant RCC cells compared with control cells. In this study, we demonstrated energy metabolism with glutamine uptake and glycolysis upregulation, as well as antioxidant activity, was also associated with sunitinib resistance in RCC cells.

Correlation of expression levels of P-glycoprotein with resistance to adriamycin in a renal adenocarcinoma cell line

1997 ◽  
Vol 25 (6) ◽  
pp. 407-412
Author(s):  
S. Kawamoto ◽  
T. Deguchi ◽  
S. Nezasa ◽  
S. Yamada ◽  
M. Okano ◽  
...  
Keyword(s):  
Cell Line ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Expression Levels ◽  
Renal Adenocarcinoma ◽  
P Glycoprotein

Very Low-Density Lipoprotein Receptor in Fetal Intestine and Gastric Adenocarcinoma Cells

2000 ◽  
Vol 124 (1) ◽  
pp. 119-122
Author(s):  
Yasuhiro Nakamura ◽  
Munehiko Yamamoto ◽  
Eriko Kumamaru
Keyword(s):  
Cell Line ◽  
Gastric Adenocarcinoma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Ags Cells ◽  
Adenocarcinoma Cells ◽  
Density Lipoprotein Receptor

Abstract Background.—A very low-density lipoprotein receptor (VLDLR) was recently identified. This receptor reportedly binds specifically to very low-density lipoproteins; however, its distribution and functions in vivo have yet to be elucidated. We investigated the expression and regulation of VLDLR in fetal and carcinoma cells. Objective.—The expression of VLDLR was examined by immunohistochemistry and reverse-transcriptase polymerase chain reaction using several specimens, including a fetus of 12 to 15 weeks' gestation, various tumors, AGS cells, and INT407 cells. Results.—Immunoreactive VLDLR was abundantly present in human fetal intestinal epithelial and gastric adenocarcinoma cells. This receptor was also noted in the intestinal cell line, INT407, and gastric cancer cell line, AGS. In addition, the VLDLR that was expressed in INT407 cells, AGS cells, and gastric adenocarcinoma tissue was present mainly in a variant form lacking the O-linked sugar domain. Conclusions.—These data suggest that an important function of VLDLR may be the mediation of cell growth in developing tissues, such as fetal intestinal and cancer cells. The INT407 and AGS cell lines appear to be useful for examining the regulation of VLDLR expression.

scholarly journals Transport systems for polyamines in the established renal cell line LLC-PK1. Polarized expression of an Na+-dependent transporter

1990 ◽  
Vol 265 (2) ◽  
pp. 609-612 ◽  
Author(s):  
L Van Den Bosch ◽  
H De Smedt ◽  
L Missiaen ◽  
J B Parys ◽  
R Borghgraef
Keyword(s):  
Cell Line ◽  
Renal Cell ◽  
The Other ◽  
Transport Systems ◽  
Present Evidence ◽  
Cell Monolayers ◽  
Other Hand ◽  
Basolateral Side ◽  
Renal Cell Line ◽  
Polyamine Uptake

We present evidence for the existence of an Na(+)-dependent transporter and an Na(+)-independent transporter for polyamines in LLC-PK1 cells. Both transporters could be discriminated by their sensitivity to inhibitors, particularly rho-chloromercuriphenyl sulphate and various polycationic molecules. By using cell monolayers grown on a permeable filter support, we have found that the Na(+)-dependent polyamine uptake occurred preferentially from the basolateral side. The Na(+)-independent uptake, on the other hand, occurred to the same extent from either the apical or the basolateral side.

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