scholarly journals Comparing the Effectiveness of Sabouraud Dextrose Agar and Dermatophytes Test Medium for Isolation of Dermatophytes

2016 ◽  
Vol 2 (4) ◽  
Author(s):  
Parameswari Katay ◽  
Sravya Ravi ◽  
Geethanjali Anke
Keyword(s):  
Test Medium ◽  
Sabouraud Dextrose Agar

scholarly journals Integument Mycobiota of Wild European Hedgehogs (Erinaceus europaeus) from Catalonia, Spain

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
R. A. Molina-López ◽  
C. Adelantado ◽  
E. L. Arosemena ◽  
E. Obón ◽  
L. Darwich ◽  
...  
Keyword(s):  
Lower Prevalence ◽  
Test Medium ◽  
Erinaceus Europaeus ◽  
Sabouraud Dextrose Agar

There are some reports about the risk of manipulating wild hedgehogs since they can be reservoirs of potential zoonotic agents like dermatophytes. The aim of this study was to describe the integument mycobiota, with special attention to dermatophytes of wild European hedgehogs. Samples from spines and fur were cultured separately in Sabouraud dextrose agar (SDA) with antibiotic and dermatophyte test medium (DTM) plates. Nineteen different fungal genera were isolated from 91 cultures of 102 hedgehogs. The most prevalent genera were Cladosporium (79.1%), Penicillium (74.7%), Alternaria (64.8%), and Rhizopus (63.7%). A lower prevalence of Aspergillus (; ) and Arthrinium (; ) was isolated during the spring time and higher frequencies of Fusarium (; ) during the autumn. The prevalence of Acremonium was significantly higher in young animals (70%, 26/37) than in adults (30%, 11/37) (; ). Moreover, the majority of the saprophytic species that grew at the SDA culture were also detected at the DTM. Finally, no cases of ringworm were diagnosed and no dermatophytes spp. were isolated. Concluding, this study provides the first description of fungal mycobiota of the integument of wild European hedgehogs in Spain, showing a large number of saprophytic species and the absence of dermatophytes.

scholarly journals CÁC CHỦNG VI NẤM NGOÀI DA PHÂN LẬP ĐƯỢC VÀ ĐỘ NHẠY CẢM VỚI CÁC THUỐC KHÁNG NẤM HIỆN NAY TRÊN BỆNH NHÂN ĐẾN KHÁM TẠI BỆNH VIỆN DA LIỄU THÀNH PHỐ HỒ CHÍ MINH NĂM 2021

2022 ◽  
Vol 508 (2) ◽  
Author(s):  
Tăng Tuấn Hải ◽  
Trần Phủ Mạnh Siêu ◽  
Ngô Quốc Đạt
Keyword(s):  
Trichophyton Rubrum ◽  
Trichophyton Mentagrophytes ◽  
Microsporum Canis ◽  
Microsporum Gypseum ◽  
Test Medium ◽  
Nam Co ◽  
Sabouraud Dextrose Agar

Đặt vấn đề: Nhiễm vi nấm ngoài da (dermatophytosis) là một trong những bệnh  phổ biến và khó điều trị. Vấn đề chẩn đoán tác nhân gây bệnh chưa được quan tâm triệt để, cộng với tình trạng kháng thuốc ngày càng tăng. Do đó cần thiết phải có nghiên cứu khảo sát các chủng vi nấm ngoài da hiện đang lưu hành trên các bệnh nhân đến khám tại bệnh viện Da Liễu, từ đó có cơ sở chẩn đoán và điều trị hiệu quả. Mục tiêu: Phân lập, định danh và tìm hiểu tỷ lệ của các chủng vi nấm ngoài da. Khảo sát độ nhạy cảm với các thuốc khám nấm hiện nay trên các bệnh nhân đến khám tại bệnh viện Da Liễu Thành phố Hồ Chí Minh. Phương pháp nghiên cứu: cắt ngang mô tả, đối tượng từ 339 mẫu bệnh phẩm da, tóc, móng nghi ngờ do vi nấm ngoài da đến khám ngoại trú tại Khoa Khám bệnh Bệnh viện Da Liễu Thành phố Hồ Chí Minh từ tháng 1 đến tháng 5 năm 2021 có chỉ định soi tươi tìm vi nấm của bác sĩ lâm sàng. Các bệnh phẩm được cấy vào môi trường Dermatophyte test medium (DTM) và Sabouraud dextrose Agar (SDA) để phân biệt và định danh. Các chủng vi nấm ngoài da được thực hiện kháng nấm bằng phương pháp đĩa khuếch tán để đánh giá hiệu lực gồm các chất kháng nấm: fluconazole, griseofulvin, itraconazole, ketoconazole. Kết quả: Tỷ lệ bệnh nhân được chẩn đoán nhiễm nấm ngoài da là 47,2%. Trên 107 mẫu bệnh phẩm nuôi cấy phân lập được vi nấm ngoài da, Trichophyton rubrum chiếm tỉ lệ cao nhất là 63,55%, kế đến là Trichophyton mentagrophytes với tỉ lệ 28,04%, Microsporum gypseum chiếm tỉ lệ 4,67%, và cuối cùng là Microsporum canis có tỉ lệ thấp nhất là 3,74%. Tất cả vi nấm ngoài da đều nhạy với thuốc kháng nấm itraconazole (100%); trong khi đó, mức độ nhạy cảm với griseofulvin là 98%. Đối với thuốc kháng nấm ketoconazole, mức độ nhạy với thuốc đạt 52,9%, và có 30,4% mẫu vi nấm ngoài da kháng với ketoconazole. Kết luận: Tỉ lệ nhiễm nấm da do vi nấm ngoài da của bệnh nhân còn cao; trong đó, loài Trichophyton rubrum là loài thường gặp nhất. Kết quả kháng nấm đồ cho thấy tình trạng đề kháng ngày càng tăng của vi nấm, có thể giảm hiệu quả điều trị.

scholarly journals Evaluation of Dermatophyte Test Medium and Sabouraud Dextrose Agar for Isolation of Dermatophyte Species

BioMedica ◽  
2020 ◽  
Vol 36 (4) ◽  
pp. 362-366
Author(s):  
Dr. Majid Rauf Ahmad ◽  
Dr. Iffat Javed ◽  
Dr. Sohaila Mushtaq ◽  
Dr. Rubeena Hafeez ◽  
Dr. Kanwal Hassan Cheema
Keyword(s):  
Culture Media ◽  
Fungal Growth ◽  
Laboratory Diagnosis ◽  
Medical Institute ◽  
Test Medium ◽  
Isolation And Identification ◽  
Time Saving ◽  
Primary Isolation ◽  
Sabouraud Dextrose Agar ◽  
Dermatophyte Species

Background and Objective: Dermatophyte infections require laboratory diagnosis before treatment is started. Although direct microscopy is routinely performed but culture of dermatophytes is the gold standard. However, it takes about 4 weeks for species identification on primary media. Our aim was to compare dermatophyte test medium (DTM) as a screening medium for the isolation of dermatophytes in comparison with sabouraud dextrose agar (SDA). Methods: It was a comparative study carried out at the Department of Microbiology of Post Graduate Medical Institute, Lahore over a period of nine months. Samples were collected from one hundred patients with clinically suspected dermatophytoses after taking informed written consent. The samples were examined microscopically and then inoculated on two types of culture media, one Sabouraud dextrose agar (SDA) with added chloramphenicol, gentacin and cycloheximide and other dermatophyte test medium (DTM) with added chlortetracycline, gentacin and cyclohexamide. Results: Fungal growth was observed in fifty-six samples on culture. Out of the fifty-six positive on cultures, nineteen were that of dermatophytes. Out of n = 100 patients, ten were positive on SDA while n = 14 dermatophyte species were able to grow on DTM. A significantly higher positivity (P ³ 0.05) for isolating dermatophytes was observed by DTM as compared to SDA. DTM was able to isolate (71%) of the dermatophytes in first 10 days. Isolation rate of dermatophyte species was higher (73.68%) on DTM as compared to SDA which was 52.6%. Conclusion: Authors recommend the use of dermatophyte test medium for the primary isolation and identification of dermatophyte species to be more effective and time saving.

Thermal Mapping, via Liquid Crystals, of the Temperature Field near a Heated Surgical Probe

1973 ◽  
Vol 95 (2) ◽  
pp. 250-256 ◽  
Author(s):  
T. E. Cooper ◽  
J. P. Groff
Keyword(s):  
Blood Flow ◽  
Temperature Field ◽  
Liquid Crystals ◽  
Theoretical Model ◽  
Wheatstone Bridge ◽  
Visual Display ◽  
Two Dimensional ◽  
Test Medium ◽  
One Dimensional ◽  
Water Test

This paper discusses the use of heat for producing clinical lesions in tissue and presents the design and analysis of a resistively heated surgical probe. The probe surface temperature is accurately maintained and controlled by using a Wheatstone bridge. The probe was embedded in a clear agar–water test medium, and the temperature field generated by the probe was measured with liquid crystals, a material that provides a visual display of certain isotherms. Experimental results compare within approximately 10 percent of a two-dimensional numerical solution. A one-dimensional theoretical model is also developed which examines the influence of blood flow on the temperature field.

Efficacy of "Dermatophyte Test Medium"

1971 ◽  
Vol 104 (5) ◽  
pp. 486 ◽  
Author(s):  
Stanley A. Rosenthal
Keyword(s):  
Test Medium

scholarly journals Paracoccidioidomicose na Amazônia. (Registro de um caso)

1972 ◽  
Vol 2 (3) ◽  
pp. 55-58 ◽  
Author(s):  
Aurélia Lopes Castrillón ◽  
Romeu Fernandes de Carvalho ◽  
Carlos Augusto Borborema ◽  
Simão Arão Pecher
Keyword(s):  
Paracoccidioides Brasiliensis ◽  
Sabouraud Dextrose Agar

Sinopse Um caso de blastomicose sul-americana em Manaus A observação micológica inicialmente usada foi o exame direto da secreção que acusou presença de células características do microrganismo. Cultura no meio padrão Sabouraud-dextrose-agar contendo antibióticos nas concentrações: cloranfenicol 50 mg./1.000 ml. de meio e ciclohexemida 250 mg/1.000 ml. de meio. Após 21 dias, evidência do Paracoccidioides brasiliensis de aspecto cotonoso. Tratamento com Fanasulf (Sulfadoxina) dois comprimidos (500 mg. cada) por semana, respondendo satisfatoriamente à droga. Depois de oito meses de medicação, o paciente recebeu alta, apresentando-se totalmente curado.

Evaluation of Czapeck agar and Sabouraud dextrose agar for the culture of airborne Aspergillus conidia

2005 ◽  
Vol 53 (4) ◽  
pp. 333-334 ◽  
Author(s):  
Jesús Guinea ◽  
Teresa Peláez ◽  
Luis Alcalá ◽  
Emilio Bouza
Keyword(s):  
Sabouraud Dextrose Agar

scholarly journals Alteration of prostaglandin production and agonist responsiveness by n-6 polyunsaturated fatty acids in endometrial cells from late-gestation ewes

2004 ◽  
Vol 182 (2) ◽  
pp. 249-256 ◽  
Author(s):  
Z Cheng ◽  
M Elmes ◽  
SE Kirkup ◽  
DR Abayasekara ◽  
DC Wathes
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Defined Medium ◽  
Mixed Population ◽  
Late Gestation ◽  
Control Medium ◽  
Test Medium ◽  
Gamma Linolenic Acid ◽  
Endometrial Cells ◽  
Term Labour

We investigated the effect of n-6 polyunsaturated fatty acids (PUFAs) on prostaglandin (PG) production by the uterus. A mixed population of endometrial cells (epthelium and stroma) from late-gestation ewes were cultured in defined medium containing linoleic acid (LA, 18:2, n-6), gamma-linolenic acid (GLA, 18:3, n-6) or arachidonic acid (AA, 20:4, n-6) in concentrations of 0 (control), 20 or 100 microM. After 45 h in test medium with or without added PUFAs, cells were challenged with control medium (CM), oxytocin (OT, 250 nM), lipopolysaccharide (LPS, 0.1 micro g/ml) or dexamethasone (DEX, 5 microM) for 22 h in the continued presence of the same concentration of PUFA and the medium was collected for measurement of PGF(2alpha) and PGE(2). Supplementation with LA inhibited the production of PGF(2alpha) but did not alter PGE(2), whereas GLA and AA increased production of both PGs. All PUFA supplements thus increased the ratio of PGE(2) to PGF(2alpha) (E:F ratio) two- to threefold. In control cells, OT and LPS challenges stimulated the production of PGF(2alpha) and PGE(2). In all challenge groups, the concentrations of PGF(2alpha) in response to PUFAs followed the same pattern - LA<control<;GLA<AA - but there were significant alterations in responsiveness as a result of PUFA treatment. In the cells supplemented with 100 microM AA, there was no further increase in PGF(2alpha) output in the presence of OT or LPS and when 100 microM GLA was present neither LPS nor OT stimulated PGE(2) significantly. When LPS was given to AA-supplemented cells, the E:F ratio was increased. DEX did not change PGE(2) production in control or LA-treated cells, but the cells produced significantly less PGF(2alpha), so the E:F ratio was increased. In contrast, in GLA- and AA-treated cells, DEX reduced the production of both PGF(2alpha) and PGE(2), so the E:F ratio was unaltered. In summary, the study showed altered production of PGs in the presence of different PUFAs according to their position in the n-6 metabolic pathway. The type of PUFA present affected responsiveness to OT, LPS and DEX and also changed the ratio of PGE(2) to PGF(2alpha) produced. The possible implications of this work are discussed in relation to the effect of diet on term and pre-term labour, which both require upregulation of the endometrial PG synthetic pathway.

Sign in / Sign up

Export Citation Format

Share Document