scholarly journals Alteration of prostaglandin production and agonist responsiveness by n-6 polyunsaturated fatty acids in endometrial cells from late-gestation ewes

2004 ◽  
Vol 182 (2) ◽  
pp. 249-256 ◽  
Author(s):  
Z Cheng ◽  
M Elmes ◽  
SE Kirkup ◽  
DR Abayasekara ◽  
DC Wathes
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Defined Medium ◽  
Mixed Population ◽  
Late Gestation ◽  
Control Medium ◽  
Test Medium ◽  
Gamma Linolenic Acid ◽  
Endometrial Cells ◽  
Term Labour

We investigated the effect of n-6 polyunsaturated fatty acids (PUFAs) on prostaglandin (PG) production by the uterus. A mixed population of endometrial cells (epthelium and stroma) from late-gestation ewes were cultured in defined medium containing linoleic acid (LA, 18:2, n-6), gamma-linolenic acid (GLA, 18:3, n-6) or arachidonic acid (AA, 20:4, n-6) in concentrations of 0 (control), 20 or 100 microM. After 45 h in test medium with or without added PUFAs, cells were challenged with control medium (CM), oxytocin (OT, 250 nM), lipopolysaccharide (LPS, 0.1 micro g/ml) or dexamethasone (DEX, 5 microM) for 22 h in the continued presence of the same concentration of PUFA and the medium was collected for measurement of PGF(2alpha) and PGE(2). Supplementation with LA inhibited the production of PGF(2alpha) but did not alter PGE(2), whereas GLA and AA increased production of both PGs. All PUFA supplements thus increased the ratio of PGE(2) to PGF(2alpha) (E:F ratio) two- to threefold. In control cells, OT and LPS challenges stimulated the production of PGF(2alpha) and PGE(2). In all challenge groups, the concentrations of PGF(2alpha) in response to PUFAs followed the same pattern - LA<control<;GLA<AA - but there were significant alterations in responsiveness as a result of PUFA treatment. In the cells supplemented with 100 microM AA, there was no further increase in PGF(2alpha) output in the presence of OT or LPS and when 100 microM GLA was present neither LPS nor OT stimulated PGE(2) significantly. When LPS was given to AA-supplemented cells, the E:F ratio was increased. DEX did not change PGE(2) production in control or LA-treated cells, but the cells produced significantly less PGF(2alpha), so the E:F ratio was increased. In contrast, in GLA- and AA-treated cells, DEX reduced the production of both PGF(2alpha) and PGE(2), so the E:F ratio was unaltered. In summary, the study showed altered production of PGs in the presence of different PUFAs according to their position in the n-6 metabolic pathway. The type of PUFA present affected responsiveness to OT, LPS and DEX and also changed the ratio of PGE(2) to PGF(2alpha) produced. The possible implications of this work are discussed in relation to the effect of diet on term and pre-term labour, which both require upregulation of the endometrial PG synthetic pathway.

Effects of n-6 polyunsaturated fatty acids on prostaglandin production in ovine fetal chorion cells in vitro in late gestation ewes

Placenta ◽  
2011 ◽  
Vol 32 (10) ◽  
pp. 752-756 ◽  
Author(s):  
Z. Cheng ◽  
M. Elmes ◽  
S. Kirkup ◽  
D.R.E. Abayasekara ◽  
D.C. Wathes
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Late Gestation ◽  
Prostaglandin Production ◽  
Ovine Fetal

scholarly journals Polyunsaturated fatty acids modulate prostaglandin synthesis by ovine amnion cells in vitro

Reproduction ◽  
2010 ◽  
Vol 140 (6) ◽  
pp. 943-951 ◽  
Author(s):  
S E Kirkup ◽  
Z Cheng ◽  
M Elmes ◽  
D C Wathes ◽  
D R E Abayasekara
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Stearidonic Acid ◽  
Prostaglandin Synthesis ◽  
Late Gestation ◽  
Prostaglandin E ◽  
Pufa Supplementation ◽  
Pufa Composition

Diets or supplements high in n-3 and n-6 polyunsaturated fatty acids (PUFAs) have been shown to influence the timing of parturition. PUFAs are substrates for prostaglandin (PG) synthesis, and PGs play central roles in parturition. Hence, the effects of altering PUFA composition may be mediated through alterations in the type and relative quantities of PGs synthesised. Therefore, we have investigated the effects of a range of n-3 and n-6 PUFAsin vitroon PG synthesis by amnion cells of late gestation ewes. The n-6 PUFA, arachidonic acid (20:4, n-6), increased synthesis of two-series PGs. Degree of stimulation induced by the n-6 PUFAs was dependent on the position of the PUFA in the PG synthetic pathway, i.e. PG production of the two-series (principally prostaglandin E2:PGE2) increased progressively with longer chain PUFAs. Effects of n-3 PUFAs on output of PGE2were more modest and variable. The two shorter chain n-3 PUFAs, α-linolenic acid (18:3, n-3) and stearidonic acid (18:4, n-3), induced a small but significant increase in PGE2output, while the longest chain n-3 PUFA docosahexaenoic acid (22:6, n-3) inhibited PGE2synthesis. Dihomo-γ-linolenic acid (20:3, n-6), the PUFA substrate for synthesis of one-series PGs, induced an increase in PGE1generation and a decrease in PGE2and PGE3outputs. Hence, we have demonstrated that PUFA supplementation of ovine amnion cellsin vitroaffects the type and quantity of PGs synthesised.

scholarly journals The influence of polyunsaturated fatty acids on the skin, featuring the effect of gamma-linolenic acid

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
Agnieszka Kaźmierska ◽  
◽  
Izabela Bolesławska ◽  
Juliusz Przysławski
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Gamma Linolenic Acid

The effects on neonatal piglet behaviour and tissue composition of feeding sows a diet containing a high level of docosahexaenoic acid (DHA) in late gestation and lactation

1997 ◽  
Vol 1997 ◽  
pp. 111-111
Author(s):  
I. M. Bland ◽  
J. A. Rooke ◽  
P. English ◽  
A. Edwards ◽  
R. C. Noble
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Brain Development ◽  
Economic Loss ◽  
Late Gestation ◽  
Early Lactation ◽  
Tissue Composition ◽  
High Level ◽  
Effect Of Feeding

Neonatal mortality is a major source of economic loss to the pig industry. Polyunsaturated fatty acids (PUFAs), especially DHA, have been implicated in brain development in man and other species and a pre-natal supply of PUFAs may have a role in influencing post-natal survival. The purpose of this study was to investigate the effect of feeding increased amounts of DHA during gestation and early lactation, on piglet viability and tissue composition.

scholarly journals Red Blood Cell Polyunsaturated Fatty Acids and Vascular Diseases: A Mendelian Randomization Study

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1494-1494
Author(s):  
Yitang Sun ◽  
Akash Ronanki ◽  
Changwei Li ◽  
Kaixiong Ye
Keyword(s):  
Fatty Acids ◽  
Heart Disease ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Mendelian Randomization ◽  
Risk Group ◽  
Causal Association ◽  
Protective Group ◽  
Gamma Linolenic Acid ◽  
Embolism And Thrombosis

Abstract Objectives To evaluate the causal association of omega-3 and omega-6 polyunsaturated fatty acids (PUFAs), as measured in red blood cells (RBC), with cardiovascular disease (CVD), cerebrovascular disease (CBVD), and peripheral vascular disease (PVD). Methods Applying a two-sample Mendelian Randomization approach, we first developed genetic instruments for RBC-PUFAs by utilizing summary statistics from previous genome-wide association study in the Framingham Heart Study. We then evaluated the association of these instrumental variables with CVD, CBVD, PVD, and their subtypes in the UK Biobank cohort. Results Strong evidence of causal association with at least one RBC-PUFAs was observed for the overall risk of PVD and three of its subtypes (aortic aneurysm and dissection, arterial embolism and thrombosis, and other PVDs), but only for two CVD subtypes (hypertensive heart disease, and chronic ischemic heart disease) and for two CBVD subtypes (stroke, and cerebral infarction). Based on their effects on all examined diseases, RBC-PUFAs clustered into two groups: a protective group with alpha-Linolenic acid (ALA), linoleic acid (LA), eicosadienoic acid (EDA), and dihomo-gamma-linolenic acid (DGLA); and the other risk group with docosapentaenoic acid (DPA), gamma-linolenic acid (GLA), arachidonic acid (AA), and adrenic acid (AdrA). PUFAs in the protective group are protective, while those in the risk group are risk-increasing, for all diseases with significant associations except for hypertensive heart diseases. In the metabolic pathway converting short-chain PUFAs into long-chain ones, the protective group is mapped to precursors of desaturases, while the risk group corresponds to their products. Conclusions Genetically regulated RBC-PUFAs are associated with the risk of PVD, and subtypes of PVD, CVD, and CBVD. Funding Sources University of Georgia Research Foundation.

Effect of Polyunsaturated Fatty Acids on Fetal Mouse Brain Cells in Culture in a Chemically Defined Medium

1983 ◽  
Vol 41 (5) ◽  
pp. 1234-1242 ◽  
Author(s):  
J. M. Bourre ◽  
A. Faivre ◽  
O. Dumont ◽  
A. Nouvelot ◽  
C. Loudes ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Mouse Brain ◽  
Defined Medium ◽  
Brain Cells ◽  
Chemically Defined Medium ◽  
Fetal Mouse ◽  
Cells In Culture

n-6 Polyunsaturated fatty acids in the feeding of late gestation hair ewes: the effects on thermoregulation, growth, and metabolism of heat-stressed growing lambs

2021 ◽  
Author(s):  
Ulises Macías-Cruz ◽  
Ricardo Vicente-Pérez ◽  
Abelardo Correa-Calderon ◽  
Miguel Mellado ◽  
Cesar A. Meza-Herrera ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Late Gestation

Fetal and maternal plasma lipids in chronically catheterized mares in late gestation: effects of different nutritional states

1995 ◽  
Vol 7 (5) ◽  
pp. 1275 ◽  
Author(s):  
JP Stammers ◽  
D Hull ◽  
M Silver ◽  
AL Fowden
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Essential Fatty Acids ◽  
Plasma Lipid ◽  
Maternal Plasma ◽  
Late Gestation ◽  
Feeding Patterns ◽  
Normal Feeding ◽  
Group Ii

The effects of different nutritional states on plasma lipid concentrations have been examined in pregnant mares and their fetuses. Maternal and fetal arterial catheters were inserted into 12 pony mares between 244-303 days' gestation (term 320-360 days) and observations made from 5 days following the insertion of catheters. After recovery from surgery maternal and fetal arterial samples were withdrawn from 7 mares with normal feeding patterns (Group IA), from four of these mares at the end of a 30 h fast and 3 h later following refeeding (Group IB) and six mares who failed to re-establish normal feeding patterns (Group II). The fatty acid concentrations and composition of the plasma free fatty acid (FFA), triacylglycerol and phospholipid fractions were analysed. Maternal FFA, triacylglycerol and phospholipid concentrations were significantly raised in the fasted (Group IB) and under-fed (Group II) mares. Fetal concentrations of FFA and phospholipid increased significantly in the group of under-fed (Group II) mares but not in the fasted (Group IB) mares. In the fetal plasma the proportions of polyunsaturated fatty acids derived from essential fatty acids in the FFA and phospholipid fractions were much higher than those in the mare. In the fasted (IB) and under-fed (II) groups the relative amounts of the polyunsaturated fatty acids in each fraction remained unchanged (P > 0.05). These results show a short fast or prolonged undernutrition result in raised maternal plasma lipid concentrations which in turn can effect the total amount of lipid in the fetal circulation. However any increases in polyunsaturated fatty acids in the fetus (e.g. in Group II) are unlikely to come from the maternal circulation; likely sources of these fatty acids are the placenta or fetal tissues.

scholarly journals The influence of polyunsaturated fatty acids on the skin, featuring the effect of gamma-linolenic acid

2017 ◽  
Vol 11 (3) ◽  
Author(s):  
Agnieszka Kaźmierska ◽  
◽  
Izabela Bolesławska ◽  
Juliusz Przysławski
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Gamma Linolenic Acid
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