Functional Characterization of a Novel Pro-Apoptotic Transcription Regulatory Protein in Ovarian Cancer

2006 ◽  
Author(s):  
Viji Shridhar
Keyword(s):  
Ovarian Cancer ◽  
Functional Characterization ◽  
Regulatory Protein

scholarly journals Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer

Oncotarget ◽  
2017 ◽  
Vol 8 (49) ◽  
pp. 85759-85771 ◽  
Author(s):  
Jia Liu ◽  
Lin Zhang ◽  
Ping Mao ◽  
Guoqiang Jiang ◽  
Likun Liu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Functional Characterization ◽  
Chemotherapy Resistance ◽  
Novel Transcript

Functional Characterization of a Fluorescent Highly Tumorigenic Ovarian Cancer Line to Test Cellular Therapy in Experimental Models

2011 ◽  
Vol 21 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Susan Blaydes Ingersoll ◽  
Sarfraz Ahmad ◽  
Gregory P. Stoltzfus ◽  
Sheylan Patel ◽  
Michael J. Radi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Polyethylene Glycol ◽  
Cell Lines ◽  
Cellular Therapy ◽  
Functional Characterization ◽  
Interleukin 2 ◽  
Experimental Models ◽  
Cancer Line ◽  
E Cadherin

ObjectivesThe objective of this study was to functionally characterize a fluorescent highly tumorigenic ovarian cancer line to test cellular therapy in combination with cytokines or chemotherapies in experimental models.MethodsA fluorescent highly tumorigenic subline (SKOV3-AF2) was derived from the SKOV3 ovarian cancer cell line. Peripheral blood mononuclear cell (PBMC)-mediated cytotoxicity of SKOV3-AF2 in the presence of interleukin 2 (IL-2) and interferon α-2b (IFNα-2b) was assayed by lactate dehydrogenase release. Sensitivity of SKOV3-AF2 cells to polyethylene glycol-IFNα-2b and IL-2 was assayed in a xenograph nude mouse model. Histopathology was performed to determine necrosis and tumor-infiltrating lymphocytes in the solid tumors. Reverse transcriptase-polymerase chain reaction was used for gene expression analyses ofE-cadherinandcysteine-rich 61(CCN1).ResultsThe SKOV3-AF2 subline exhibits increased cytotoxicity (up to 70%), mediated by PBMCs, IL-2, and IFNα-2b, compared with parental SKOV3-red fluorescent protein (RFP) cells. SKOV3-AF2 cells are more tumorigenic in vivo as indicated by tumor incidence, time to sacrifice, tumor weight, and ascitic fluid production. SKOV3-AF2 tumor growth was inhibited by polyethylene glycol-IFNα-2b but not low-dose IL-2. Histopathology revealed that the tumors consisted of poorly differentiated surface epithelial carcinoma. SKOV3-RFP, and -AF2 cell lines as well as -AF2 tumors expressedE-cadherin.SKOV3-AF2 derived tumors expressedCCN1; however, the SKOV3-RFP and SKOV3-AF2 cell lines did not.ConclusionsCharacterization of SKOV3-AF2 cells revealed that it is more susceptible to PBMC-mediated cytotoxicity than SKOV3-RFP and highly tumorigenic in a xenograph model, and AF-2 tumors express genes that promote aggressive behavior. Collectively, our data suggest that the SKOV3-AF2 subline will be a useful tool to test cellular therapy for the treatment of ovarian cancer utilizing experimental models.

scholarly journals Fabrication and functional characterization of goldnanoconjugates for potential application in ovarian cancer

2010 ◽  
Vol 20 (3) ◽  
pp. 547-554 ◽  
Author(s):  
Chitta Ranjan Patra ◽  
Resham Bhattacharya ◽  
Priyabrata Mukherjee
Keyword(s):  
Ovarian Cancer ◽  
Potential Application ◽  
Functional Characterization

Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation

2007 ◽  
Vol 5 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Christiana DelloRusso ◽  
Piri L. Welcsh ◽  
Weixin Wang ◽  
Rochelle L. Garcia ◽  
Mary-Claire King ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Ionizing Radiation ◽  
Cell Line ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Ovarian Cancer Cell Line ◽  
Functional Characterization ◽  
Cancer Cell Line

scholarly journals Structural and Functional Characterization of a Novel T Cell Receptor Co-regulatory Protein Complex, CD97-CD55

2007 ◽  
Vol 282 (30) ◽  
pp. 22023-22032 ◽  
Author(s):  
Rachel J. M. Abbott ◽  
Ian Spendlove ◽  
Pietro Roversi ◽  
Hannah Fitzgibbon ◽  
Vroni Knott ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Protein Complex ◽  
Functional Characterization ◽  
Regulatory Protein ◽  
Cell Receptor
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