Functional Characterization of a Novel Pro-Apoptotic Transcriptional Regulatory Protein in Ovarian Cancer

2005 ◽  
Author(s):  
Viji Shridhar
Keyword(s):  
Ovarian Cancer ◽  
Functional Characterization ◽  
Regulatory Protein ◽  
Transcriptional Regulatory

scholarly journals Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer

Oncotarget ◽  
2017 ◽  
Vol 8 (49) ◽  
pp. 85759-85771 ◽  
Author(s):  
Jia Liu ◽  
Lin Zhang ◽  
Ping Mao ◽  
Guoqiang Jiang ◽  
Likun Liu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Functional Characterization ◽  
Chemotherapy Resistance ◽  
Novel Transcript

Functional Characterization of a Fluorescent Highly Tumorigenic Ovarian Cancer Line to Test Cellular Therapy in Experimental Models

2011 ◽  
Vol 21 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Susan Blaydes Ingersoll ◽  
Sarfraz Ahmad ◽  
Gregory P. Stoltzfus ◽  
Sheylan Patel ◽  
Michael J. Radi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Polyethylene Glycol ◽  
Cell Lines ◽  
Cellular Therapy ◽  
Functional Characterization ◽  
Interleukin 2 ◽  
Experimental Models ◽  
Cancer Line ◽  
E Cadherin

ObjectivesThe objective of this study was to functionally characterize a fluorescent highly tumorigenic ovarian cancer line to test cellular therapy in combination with cytokines or chemotherapies in experimental models.MethodsA fluorescent highly tumorigenic subline (SKOV3-AF2) was derived from the SKOV3 ovarian cancer cell line. Peripheral blood mononuclear cell (PBMC)-mediated cytotoxicity of SKOV3-AF2 in the presence of interleukin 2 (IL-2) and interferon α-2b (IFNα-2b) was assayed by lactate dehydrogenase release. Sensitivity of SKOV3-AF2 cells to polyethylene glycol-IFNα-2b and IL-2 was assayed in a xenograph nude mouse model. Histopathology was performed to determine necrosis and tumor-infiltrating lymphocytes in the solid tumors. Reverse transcriptase-polymerase chain reaction was used for gene expression analyses ofE-cadherinandcysteine-rich 61(CCN1).ResultsThe SKOV3-AF2 subline exhibits increased cytotoxicity (up to 70%), mediated by PBMCs, IL-2, and IFNα-2b, compared with parental SKOV3-red fluorescent protein (RFP) cells. SKOV3-AF2 cells are more tumorigenic in vivo as indicated by tumor incidence, time to sacrifice, tumor weight, and ascitic fluid production. SKOV3-AF2 tumor growth was inhibited by polyethylene glycol-IFNα-2b but not low-dose IL-2. Histopathology revealed that the tumors consisted of poorly differentiated surface epithelial carcinoma. SKOV3-RFP, and -AF2 cell lines as well as -AF2 tumors expressedE-cadherin.SKOV3-AF2 derived tumors expressedCCN1; however, the SKOV3-RFP and SKOV3-AF2 cell lines did not.ConclusionsCharacterization of SKOV3-AF2 cells revealed that it is more susceptible to PBMC-mediated cytotoxicity than SKOV3-RFP and highly tumorigenic in a xenograph model, and AF-2 tumors express genes that promote aggressive behavior. Collectively, our data suggest that the SKOV3-AF2 subline will be a useful tool to test cellular therapy for the treatment of ovarian cancer utilizing experimental models.

scholarly journals Expression Characteristics and Functional Analysis of the ScWRKY3 Gene from Sugarcane

2018 ◽  
Vol 19 (12) ◽  
pp. 4059 ◽  
Author(s):  
Ling Wang ◽  
Feng Liu ◽  
Xu Zhang ◽  
Wenju Wang ◽  
Tingting Sun ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Functional Characterization ◽  
Bacterial Pathogen ◽  
Future Research ◽  
Sporisorium Scitamineum ◽  
Transcriptional Regulatory ◽  
Transient Overexpression ◽  
Hybrid Cultivar ◽  
Sugarcane Stem

The plant-specific WRKY transcriptional regulatory factors have been proven to play vital roles in plant growth, development, and responses to biotic and abiotic stresses. However, there are few studies on the WRKY gene family in sugarcane (Saccharum spp.). In the present study, the characterization of a new subgroup, IIc WRKY protein ScWRKY3, from a Saccharum hybrid cultivar is reported. The ScWRKY3 protein was localized in the nucleus of Nicotiana benthamiana leaves and showed no transcriptional activation activity and no toxic effects on the yeast strain Y2HGold. An interaction between ScWRKY3 and a reported sugarcane protein ScWRKY4, was confirmed in the nucleus. The ScWRKY3 gene had the highest expression level in sugarcane stem pith. The transcript of ScWRKY3 was stable in the smut-resistant Saccharum hybrid cultivar Yacheng05-179, while it was down-regulated in the smut-susceptible Saccharum hybrid cultivar ROC22 during inoculation with the smut pathogen (Sporisorium scitamineum) at 0–72 h. ScWRKY3 was remarkably up-regulated by sodium chloride (NaCl), polyethylene glycol (PEG), and plant hormone abscisic acid (ABA), but it was down-regulated by salicylic acid (SA) and methyl jasmonate (MeJA). Moreover, transient overexpression of the ScWRKY3 gene in N. benthamiana indicated a negative regulation during challenges with the fungal pathogen Fusarium solani var. coeruleum or the bacterial pathogen Ralstonia solanacearum in N. benthamiana. The findings of the present study should accelerate future research on the identification and functional characterization of the WRKY family in sugarcane.

scholarly journals Gene Cloning and Characterization of Multiple Alkane Hydroxylase Systems in Rhodococcus Strains Q15 and NRRL B-16531

2002 ◽  
Vol 68 (12) ◽  
pp. 5933-5942 ◽  
Author(s):  
L. G. Whyte ◽  
T. H. M. Smits ◽  
D. Labbé ◽  
B. Witholt ◽  
C. W. Greer ◽  
...  
Keyword(s):  
Regulatory Protein ◽  
Gene Clusters ◽  
Alkane Hydroxylase ◽  
Hydrophobic Membrane ◽  
Transcriptional Regulatory ◽  
Monooxygenase Gene ◽  
Alkane Monooxygenase ◽  
Membrane Spanning ◽  
Degradative Enzyme

ABSTRACT The alkane hydroxylase systems of two Rhodococcus strains (NRRL B-16531 and Q15, isolated from different geographical locations) were characterized. Both organisms contained at least four alkane monooxygenase gene homologs (alkB1, alkB2, alkB3, and alkB4). In both strains, the alkB1 and alkB2 homologs were part of alk gene clusters, each encoding two rubredoxins (rubA1 and rubA2; rubA3 and rubA4), a putative TetR transcriptional regulatory protein (alkU1; alkU2), and, in the alkB1 cluster, a rubredoxin reductase (rubB). The alkB3 and alkB4 homologs were found as separate genes which were not part of alk gene clusters. Functional heterologous expression of some of the rhodococcal alk genes (alkB2, rubA2, and rubA4 [NRRL B-16531]; alkB2 and rubB [Q15]) was achieved in Escherichia coli and Pseudomonas expression systems. Pseudomonas recombinants containing rhodococcal alkB2 were able to mineralize and grow on C12 to C16 n-alkanes. All rhodococcal alkane monooxygenases possessed the highly conserved eight-histidine motif, including two apparent alkane monooxygenase signature motifs (LQRH[S/A]DHH and NYXEHYG[L/M]), and the six hydrophobic membrane-spanning regions found in all alkane monooxygenases related to the Pseudomonas putida GPo1 alkane monooxygenase. The presence of multiple alkane hydroxylases in the two rhodococcal strains is reminiscent of other multiple-degradative-enzyme systems reported in Rhodococcus.

scholarly journals Fabrication and functional characterization of goldnanoconjugates for potential application in ovarian cancer

2010 ◽  
Vol 20 (3) ◽  
pp. 547-554 ◽  
Author(s):  
Chitta Ranjan Patra ◽  
Resham Bhattacharya ◽  
Priyabrata Mukherjee
Keyword(s):  
Ovarian Cancer ◽  
Potential Application ◽  
Functional Characterization

Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation

2007 ◽  
Vol 5 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Christiana DelloRusso ◽  
Piri L. Welcsh ◽  
Weixin Wang ◽  
Rochelle L. Garcia ◽  
Mary-Claire King ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Ionizing Radiation ◽  
Cell Line ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Ovarian Cancer Cell Line ◽  
Functional Characterization ◽  
Cancer Cell Line
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