scholarly journals Functional characterization of a novel transcript of ERCC1 in chemotherapy resistance of ovarian cancer

Oncotarget ◽  
2017 ◽  
Vol 8 (49) ◽  
pp. 85759-85771 ◽  
Author(s):  
Jia Liu ◽  
Lin Zhang ◽  
Ping Mao ◽  
Guoqiang Jiang ◽  
Likun Liu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Functional Characterization ◽  
Chemotherapy Resistance ◽  
Novel Transcript

Functional Characterization of a Fluorescent Highly Tumorigenic Ovarian Cancer Line to Test Cellular Therapy in Experimental Models

2011 ◽  
Vol 21 (3) ◽  
pp. 457-465 ◽  
Author(s):  
Susan Blaydes Ingersoll ◽  
Sarfraz Ahmad ◽  
Gregory P. Stoltzfus ◽  
Sheylan Patel ◽  
Michael J. Radi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Polyethylene Glycol ◽  
Cell Lines ◽  
Cellular Therapy ◽  
Functional Characterization ◽  
Interleukin 2 ◽  
Experimental Models ◽  
Cancer Line ◽  
E Cadherin

ObjectivesThe objective of this study was to functionally characterize a fluorescent highly tumorigenic ovarian cancer line to test cellular therapy in combination with cytokines or chemotherapies in experimental models.MethodsA fluorescent highly tumorigenic subline (SKOV3-AF2) was derived from the SKOV3 ovarian cancer cell line. Peripheral blood mononuclear cell (PBMC)-mediated cytotoxicity of SKOV3-AF2 in the presence of interleukin 2 (IL-2) and interferon α-2b (IFNα-2b) was assayed by lactate dehydrogenase release. Sensitivity of SKOV3-AF2 cells to polyethylene glycol-IFNα-2b and IL-2 was assayed in a xenograph nude mouse model. Histopathology was performed to determine necrosis and tumor-infiltrating lymphocytes in the solid tumors. Reverse transcriptase-polymerase chain reaction was used for gene expression analyses ofE-cadherinandcysteine-rich 61(CCN1).ResultsThe SKOV3-AF2 subline exhibits increased cytotoxicity (up to 70%), mediated by PBMCs, IL-2, and IFNα-2b, compared with parental SKOV3-red fluorescent protein (RFP) cells. SKOV3-AF2 cells are more tumorigenic in vivo as indicated by tumor incidence, time to sacrifice, tumor weight, and ascitic fluid production. SKOV3-AF2 tumor growth was inhibited by polyethylene glycol-IFNα-2b but not low-dose IL-2. Histopathology revealed that the tumors consisted of poorly differentiated surface epithelial carcinoma. SKOV3-RFP, and -AF2 cell lines as well as -AF2 tumors expressedE-cadherin.SKOV3-AF2 derived tumors expressedCCN1; however, the SKOV3-RFP and SKOV3-AF2 cell lines did not.ConclusionsCharacterization of SKOV3-AF2 cells revealed that it is more susceptible to PBMC-mediated cytotoxicity than SKOV3-RFP and highly tumorigenic in a xenograph model, and AF-2 tumors express genes that promote aggressive behavior. Collectively, our data suggest that the SKOV3-AF2 subline will be a useful tool to test cellular therapy for the treatment of ovarian cancer utilizing experimental models.

scholarly journals Fabrication and functional characterization of goldnanoconjugates for potential application in ovarian cancer

2010 ◽  
Vol 20 (3) ◽  
pp. 547-554 ◽  
Author(s):  
Chitta Ranjan Patra ◽  
Resham Bhattacharya ◽  
Priyabrata Mukherjee
Keyword(s):  
Ovarian Cancer ◽  
Potential Application ◽  
Functional Characterization

scholarly journals New insights into Arabidopsis transcriptome complexity revealed by direct sequencing of native RNAs

2020 ◽  
Vol 48 (14) ◽  
pp. 7700-7711 ◽  
Author(s):  
Shoudong Zhang ◽  
Runsheng Li ◽  
Li Zhang ◽  
Shengjie Chen ◽  
Min Xie ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Transcription Initiation ◽  
Direct Sequencing ◽  
Functional Characterization ◽  
Read Length ◽  
Long Distance ◽  
Transcript Isoforms ◽  
Fusion Transcripts ◽  
Novel Transcript

Abstract Arabidopsis thaliana transcriptomes have been extensively studied and characterized under different conditions. However, most of the current ‘RNA-sequencing’ technologies produce a relatively short read length and demand a reverse-transcription step, preventing effective characterization of transcriptome complexity. Here, we performed Direct RNA Sequencing (DRS) using the latest Oxford Nanopore Technology (ONT) with exceptional read length. We demonstrate that the complexity of the A. thaliana transcriptomes has been substantially under-estimated. The ONT direct RNA sequencing identified novel transcript isoforms at both the vegetative (14-day old seedlings, stage 1.04) and reproductive stages (stage 6.00–6.10) of development. Using in-house software called TrackCluster, we determined alternative transcription initiation (ATI), alternative polyadenylation (APA), alternative splicing (AS), and fusion transcripts. More than 38 500 novel transcript isoforms were identified, including six categories of fusion-transcripts that may result from differential RNA processing mechanisms. Aided by the Tombo algorithm, we found an enrichment of m5C modifications in the mobile mRNAs, consistent with a recent finding that m5C modification in mRNAs is crucial for their long-distance movement. In summary, ONT DRS offers an advantage in the identification and functional characterization of novel RNA isoforms and RNA base modifications, significantly improving annotation of the A. thaliana genome.

Functional Characterization of a Novel BRCA1-Null Ovarian Cancer Cell Line in Response to Ionizing Radiation

2007 ◽  
Vol 5 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Christiana DelloRusso ◽  
Piri L. Welcsh ◽  
Weixin Wang ◽  
Rochelle L. Garcia ◽  
Mary-Claire King ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Ionizing Radiation ◽  
Cell Line ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Ovarian Cancer Cell Line ◽  
Functional Characterization ◽  
Cancer Cell Line

Functional characterization of a tumor suppressor micro-RNA in ovarian cancer

2016 ◽  
Vol 61 ◽  
pp. S106
Author(s):  
B. Majem ◽  
A. Parrilla ◽  
A. Soriano ◽  
A. Gil-Moreno ◽  
M.F. Segura ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Tumor Suppressor ◽  
Functional Characterization ◽  
Micro Rna
Sign in / Sign up

Export Citation Format

Share Document