An ALI culture well exhibiting well-differentiated respiratory epithelium with ciliary activity

ASVIDE ◽  
2019 ◽  
Vol 6 ◽  
pp. 63-63
Author(s):  
Jesse A. Ende ◽  
Sejad Ahmadzada ◽  
Jenna M. Christensen ◽  
Brian Oliver ◽  
Tom Estephan ◽  
...  
Keyword(s):  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Well Differentiated

An ALI culture well with well-differentiated respiratory epithelium demonstrating coordinated ciliary activity and mucociliary clearance

ASVIDE ◽  
2019 ◽  
Vol 6 ◽  
pp. 64-64
Author(s):  
Jesse A. Ende ◽  
Sejad Ahmadzada ◽  
Jenna M. Christensen ◽  
Brian Oliver ◽  
Tom Estepha ◽  
...  
Keyword(s):  
Mucociliary Clearance ◽  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Well Differentiated

beta-Adrenergic stimulation of respiratory ciliary activity

1980 ◽  
Vol 48 (5) ◽  
pp. 868-871 ◽  
Author(s):  
P. Verdugo ◽  
N. T. Johnson ◽  
P. Y. Tam
Keyword(s):  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Ciliated Cells ◽  
Adrenergic Stimulation ◽  
Laser Scattering ◽  
Beta Adrenergic ◽  
Ciliary Beating ◽  
Stimulation Of

We investigated the effect of isoproterenol on ciliary activity using a mucus-free preparation of cultured ciliated cells of the rabbit trachea. The frequency of ciliary beating was monitored by dynamic laser-scattering spectroscopy. The results demonstrated that isoproterenol directly stimulates the activity of ciliated cells of the respiratory epithelium and that this effect is beta-adrenergic specific inasmuch as the observed stimulation can be blocked by propranolol.

scholarly journals THE LOCALIZATION OF SUCCINIC DEHYDROGENASE, CYTOCHROME OXIDASE AND ADENOSINE TRIPHOSPHATASE IN CILIATED RESPIRATORY EPITHELIUM

1965 ◽  
Vol 13 (8) ◽  
pp. 677-683 ◽  
Author(s):  
HERTHA R. CRESS ◽  
ALEXANDER SPOCK ◽  
DUNCAN C. HEATHERINGTON
Keyword(s):  
Cytochrome Oxidase ◽  
Adenosine Triphosphatase ◽  
Succinic Dehydrogenase ◽  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Basal Bodies ◽  
Tissue Cultures ◽  
Respiratory Epithelial Cells ◽  
Respiratory Epithelial

Succinic dehydrogenase and cytochrome oxidase activities were found to be scattered throughout the cytoplasm of ciliated and nonciliated respiratory epithelial cells while ATPase activity was restricted to cilia and areas under the cilia in the regions of the ciliary basal bodies. In order to elucidate the role of ATPase further, tissue cultures of rabbit tracheal epithelium with beating cilia were incubated in a medium perfused with cigarette smoke which resulted in cessation of ciliary motility. Epithelium with beating cilia was positive for ATPase while the epithelium with nonbeating cilia was negative or only weakly positive in a few small scattered areas. The presence of ATPase in beating cilia and its absence in nonbeating cilia agree with biochemical and physiological studies suggesting an association between ATP and ciliary activity.

Video image analysis of ciliary activity of human respiratory epithelium in culture

2003 ◽  
Author(s):  
E. Lamiot ◽  
J.-M. Zahm ◽  
D. Pierrot ◽  
M. Chevillard ◽  
J. Hinnrasky ◽  
...  
Keyword(s):  
Image Analysis ◽  
Respiratory Epithelium ◽  
Video Image ◽  
Ciliary Activity ◽  
Video Image Analysis

scholarly journals Transduction of Well-Differentiated Airway Epithelium by Recombinant Adeno-Associated Virus Is Limited by Vector Entry

1999 ◽  
Vol 73 (7) ◽  
pp. 6085-6088 ◽  
Author(s):  
Robert Bals ◽  
Weidong Xiao ◽  
Nianli Sang ◽  
Daniel J. Weiner ◽  
Rupalie L. Meegalla ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
Respiratory Epithelium ◽  
Transduction Efficiency ◽  
Physical Barrier ◽  
Airway Epithelia ◽  
Adeno Associated Virus ◽  
Differentiated Cells ◽  
Undifferentiated Cells ◽  
Well Differentiated ◽  
Air Liquid Interface

ABSTRACT The limitations of adeno-associated virus (AAV)-mediated vectors for lung-directed gene transfer were investigated by using differentiated human respiratory epithelium in air-liquid interface cultures. Transduction efficiency was high in undifferentiated cells and was enhanced in well-differentiated cells after basolateral application of the vector or after apical application following disruption of tight junctions or pretreatment of the cultures with glycosidases. These results indicate that transduction of airway epithelia by AAV vectors is limited by entry and reinforce the importance of a physical barrier on the airway surface.

scholarly journals Effects of Long-Acting Muscarinic Antagonists on Promoting Ciliary Function in Airway Epithelium

2022 ◽  
Author(s):  
Mineo Katsumata ◽  
Tomoyuki Fujisawa ◽  
Yosuke Kamiya ◽  
Yuko Tanaka ◽  
Chiaki Kamiya ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
Defense Mechanism ◽  
Beat Frequency ◽  
Chronic Obstructive ◽  
Ciliary Activity ◽  
Muscarinic Antagonists ◽  
Ciliary Function ◽  
Recovery Stroke ◽  
Well Differentiated ◽  
Long Acting

Abstract Background Mucociliary clearance (MCC) is an essential defense mechanism in airway epithelia for removing pathogens from the respiratory tract. Impaired ciliary functions and MCC have been demonstrated in asthma and chronic obstructive pulmonary disease (COPD). Long-acting muscarinic antagonists (LAMAs) are a major class of inhaled bronchodilators, which are used for treating asthma and COPD; however, the effects of LAMAs on ciliary function remain unclear. This study aimed to identify the effects of LAMAs on airway ciliary functions. Methods Wild-type BALB/c mice were treated with daily intranasal administrations of glycopyrronium for 7 days, and tracheal samples were collected. Cilia-driven flow and ciliary activity, including ciliary beat frequency (CBF), ciliary beating amplitude, effective stroke velocity, recovery stroke velocity and the ratio of effective stroke velocity to recovery stroke velocity, were analyzed by imaging techniques. Using in vitro murine models, tracheal tissues were transiently cultured in media with/without LAMAs, glycopyrronium or tiotropium, for 60 min. Cilia-driven flow and ciliary activity were then analyzed. Well-differentiated normal human bronchial epithelial (NHBE) cells were treated with glycopyrronium, tiotropium, or vehicle for 60 min, and CBF was evaluated. Several mechanistic analyses were performed. Results Intranasal glycopyrronium administration for 7 days significantly increased cilia-driven flow and ciliary activity in murine airway epithelium. In the murine tracheal organ culture models, treatment with glycopyrronium or tiotropium for 60 min significantly increased cilia-driven flow and ciliary activity in airway epithelium. Further, we confirmed that 60-min treatment with glycopyrronium or tiotropium directly increased CBF in well-differentiated NHBE cells. In the mechanistic analyses, neither treatment with glycopyrronium nor tiotropium affected intracellular calcium ion concentrations in well-differentiated NHBE cells. Glycopyrronium did not increase protein kinase A activity in well-differentiated NHBE cells. Moreover, glycopyrronium had no effect on extracellular adenosine triphosphate concentration. Conclusions LAMAs exert a direct effect on airway epithelium to enhance ciliary function, which may improve impaired MCC in asthma and COPD. Further investigations are warranted to elucidate the underlying mechanisms of the effects of LAMAs on the promotion of airway ciliary function.

scholarly journals Effects of Long-acting Muscarinic Antagonists on Promoting Ciliary Function in Airway Epithelium

2021 ◽  
Author(s):  
Mineo Katsumata ◽  
Tomoyuki Fujisawa ◽  
Yosuke Kamiya ◽  
Yuko Tanaka ◽  
Chiaki Kamiya ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
Defense Mechanism ◽  
Beat Frequency ◽  
Chronic Obstructive ◽  
Ciliary Activity ◽  
Muscarinic Antagonists ◽  
Ciliary Function ◽  
Recovery Stroke ◽  
Well Differentiated ◽  
Long Acting

Abstract BackgroundMucociliary clearance (MCC) is an essential defense mechanism in airway epithelia for removing pathogens from the respiratory tract. Impaired ciliary functions and MCC have been demonstrated in asthma and chronic obstructive pulmonary disease (COPD). Long-acting muscarinic antagonists (LAMAs) are a major class of inhaled bronchodilators, which are used for treating asthma and COPD; however, the effects of LAMAs on ciliary function remain unclear. This study aimed to identify the effects of LAMAs on airway ciliary functions.MethodsWild-type BALB/c mice were treated with daily intranasal administrations of glycopyrronium for 7 days, and tracheal samples were collected. Cilia-driven flow and ciliary activity, including ciliary beat frequency (CBF), ciliary beating amplitude, effective stroke velocity, recovery stroke velocity and the ratio of effective stroke velocity to recovery stroke velocity, were analyzed by imaging techniques. Using in vitro murine models, tracheal tissues were transiently cultured in media with/without LAMAs, glycopyrronium or tiotropium, for 60 min. Cilia-driven flow and ciliary activity were then analyzed. Well-differentiated normal human bronchial epithelial (NHBE) cells were treated with glycopyrronium, tiotropium, or vehicle for 60 min, and CBF was evaluated. Several mechanistic analyses were performed.ResultsIntranasal glycopyrronium administration for 7 days significantly increased cilia-driven flow and ciliary activity in murine airway epithelium. In the murine tracheal organ culture models, treatment with glycopyrronium or tiotropium for 60 min significantly increased cilia-driven flow and ciliary activity in airway epithelium. Further, we confirmed that 60-min treatment with glycopyrronium or tiotropium directly increased CBF in well-differentiated NHBE cells. In the mechanistic analyses, neither treatment with glycopyrronium nor tiotropium affected intracellular calcium ion concentrations in well-differentiated NHBE cells. Glycopyrronium did not increase protein kinase A activity in well-differentiated NHBE cells. Moreover, glycopyrronium had no effect on extracellular adenosine triphosphate concentration.ConclusionsLAMAs exert a direct effect on airway epithelium to enhance ciliary function, which may improve impaired MCC in asthma and COPD. Further investigations are warranted to elucidate the underlying mechanisms of the effects of LAMAs on the promotion of airway ciliary function.

Morphology and behaviour of the benthic larva of Arenicola cristata (Polychaeta)

1978 ◽  
Vol 56 (2) ◽  
pp. 224-237 ◽  
Author(s):  
Joan R. Marsden ◽  
T. Lacalli
Keyword(s):  
Small Region ◽  
Cholinergic Innervation ◽  
Ciliary Activity ◽  
Apical Region ◽  
Normal Behaviour ◽  
Arenicola Cristata ◽  
Glandular Cells ◽  
Muscarinic Cholinergic ◽  
Well Differentiated ◽  
Longitudinal Muscles

The structure of the three-setiger larva of Arenicola cristata is described with emphasis on the ultrastructure of the apical region, particularly the nervous and muscular systems, and ciliated and glandular cells. At least one population of well-differentiated nerve cell bodies can be identified in the cerebral ganglia, and these are filled with dense-cored vesicles. Processes apparently derived from these cells, containing both dense-cored and clear vesicles, are found throughout the cerebral commissure and circumesophageal connectives. Nerve terminals are restricted to a single small region along each of the two ventrolateral longitudinal muscles. Most of the terminals contain predominantly one or the other of the two vesicle types, but some terminals appear to contain both. Normal behaviour involves control of both muscular and ciliary activities. Experiments with drugs indicate a muscarinic cholinergic innervation of muscle, but we have found neither pharmacological nor structural evidence for a mechanism controlling ciliary activity. Preliminary observations suggest the presence of biogenic amines exhibiting specific fluorescence and a relationship between serotonin and the secretion of mucus.

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