An ALI culture well with well-differentiated respiratory epithelium demonstrating coordinated ciliary activity and mucociliary clearance

ASVIDE ◽  
2019 ◽  
Vol 6 ◽  
pp. 64-64
Author(s):  
Jesse A. Ende ◽  
Sejad Ahmadzada ◽  
Jenna M. Christensen ◽  
Brian Oliver ◽  
Tom Estepha ◽  
...  
Keyword(s):  
Mucociliary Clearance ◽  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Well Differentiated

An ALI culture well exhibiting well-differentiated respiratory epithelium with ciliary activity

ASVIDE ◽  
2019 ◽  
Vol 6 ◽  
pp. 63-63
Author(s):  
Jesse A. Ende ◽  
Sejad Ahmadzada ◽  
Jenna M. Christensen ◽  
Brian Oliver ◽  
Tom Estephan ◽  
...  
Keyword(s):  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Well Differentiated

Sinonasal effects of external dacryocystorhinostomy

2021 ◽  
pp. 1-4
Author(s):  
H Eyigor ◽  
E A Cetinkaya ◽  
D T Coban ◽  
G Ozturk ◽  
Ö Erdem
Keyword(s):  
Mucociliary Clearance ◽  
Nasal Symptom ◽  
Ciliary Activity ◽  
Test Results ◽  
Saccharine Test ◽  
Significant Difference ◽  
Symptom Scores ◽  
Endoscopic Score ◽  
External Dacryocystorhinostomy

Abstract Objective External dacryocystorhinostomy is thought to cause mucociliary dysfunction by damaging the mucosa, in turn affecting ciliary activity and mucus quality. This study investigated the effect of external dacryocystorhinostomy on sinonasal function. Methods Patients scheduled for unilateral external dacryocystorhinostomy who underwent endoscopic nasal examination and paranasal sinus computed tomography were included in this study. A saccharine test was performed on the planned surgical side and the mucociliary clearance time was determined. The sinonasal quality of life was measured in all patients, pre-operatively and at six months post-operatively, using the Sino-Nasal Outcome Test-22. The Lund–Kennedy endoscopic score was also determined in all patients, both pre- and post-operatively. Results The study comprised 28 patients (22 females and 6 males). A statistically significant difference was found between the pre- and post-operative saccharine test results (p = 0.006), but not between the pre- and post-operative Sino-Nasal Outcome Test-22 scores (p > 0.05). Conclusion This study is one of only a few to investigate the effect of external dacryocystorhinostomy on sinonasal function. The results showed that external dacryocystorhinostomy impairs mucociliary clearance. The surgical procedure is well tolerated and does not significantly change nasal symptom scores.

scholarly journals Evaluation of nasal mucociliary clearance in rheumatoid arthritis: a comparative analysis using saccharin test

2017 ◽  
Vol 5 (11) ◽  
pp. 5026 ◽  
Author(s):  
Shelja Deswal ◽  
Jyoti Yadav ◽  
Mohit Deswal ◽  
Harpreet Singh
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Duration ◽  
Mucociliary Clearance ◽  
Systemic Disease ◽  
Unknown Etiology ◽  
Control Group ◽  
Ciliary Activity ◽  
Multisystem Disease ◽  
Organic Particles ◽  
Nasal Mucociliary Clearance

Background: Rheumatoid arthritis (RA) is a chronic multisystem disease of unknown etiology characterized by persistent inflammatory synovitis, usually involving peripheral joints in a symmetric distribution. RA is a systemic disease often associated with cutaneous and organ-specific extra-articular manifestations the mucociliary clearance system protects the lower respiratory system by trapping and removing inhaled pathogenic viruses and bacteria, in addition to nontoxic and toxic particulates (e.g., pollen, ash, mineral dust, mold spores, and organic particles), from the lungs. Effective clearance requires both ciliary activity and the appropriate balance of periciliary fluid and mucus.Methods: This was a case control study conducted in the Department of Physiology, Pt. B.D. Sharma PGIMS, Rohtak in 50 females of age group 30-50 years. Control group comprised of 25 healthy volunteer females while study group comprised of 25 rheumatoid arthritis female patients with disease duration of more than five years. Proven cases of RA (as per 1987 ACR criteria) were taken with disease duration of more than five years from Rheumatology clinic of Pt. B.D. Sharma PGIMS, Rohtak. Nasal mucociliary clearance time was evaluated by saccharin method.Results: The results of our study showed abnormal mucociliary clearance in rheumatoid arthritis patients.Conclusions: The study shows an abnormal mucociliary clearance in rheumatoid arthritis patients. Impairment of mucociliary clearance seems to be the result of qualitative and quantitative alterations in respiratory secretions.

beta-Adrenergic stimulation of respiratory ciliary activity

1980 ◽  
Vol 48 (5) ◽  
pp. 868-871 ◽  
Author(s):  
P. Verdugo ◽  
N. T. Johnson ◽  
P. Y. Tam
Keyword(s):  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Ciliated Cells ◽  
Adrenergic Stimulation ◽  
Laser Scattering ◽  
Beta Adrenergic ◽  
Ciliary Beating ◽  
Stimulation Of

We investigated the effect of isoproterenol on ciliary activity using a mucus-free preparation of cultured ciliated cells of the rabbit trachea. The frequency of ciliary beating was monitored by dynamic laser-scattering spectroscopy. The results demonstrated that isoproterenol directly stimulates the activity of ciliated cells of the respiratory epithelium and that this effect is beta-adrenergic specific inasmuch as the observed stimulation can be blocked by propranolol.

scholarly journals THE LOCALIZATION OF SUCCINIC DEHYDROGENASE, CYTOCHROME OXIDASE AND ADENOSINE TRIPHOSPHATASE IN CILIATED RESPIRATORY EPITHELIUM

1965 ◽  
Vol 13 (8) ◽  
pp. 677-683 ◽  
Author(s):  
HERTHA R. CRESS ◽  
ALEXANDER SPOCK ◽  
DUNCAN C. HEATHERINGTON
Keyword(s):  
Cytochrome Oxidase ◽  
Adenosine Triphosphatase ◽  
Succinic Dehydrogenase ◽  
Respiratory Epithelium ◽  
Ciliary Activity ◽  
Basal Bodies ◽  
Tissue Cultures ◽  
Respiratory Epithelial Cells ◽  
Respiratory Epithelial

Succinic dehydrogenase and cytochrome oxidase activities were found to be scattered throughout the cytoplasm of ciliated and nonciliated respiratory epithelial cells while ATPase activity was restricted to cilia and areas under the cilia in the regions of the ciliary basal bodies. In order to elucidate the role of ATPase further, tissue cultures of rabbit tracheal epithelium with beating cilia were incubated in a medium perfused with cigarette smoke which resulted in cessation of ciliary motility. Epithelium with beating cilia was positive for ATPase while the epithelium with nonbeating cilia was negative or only weakly positive in a few small scattered areas. The presence of ATPase in beating cilia and its absence in nonbeating cilia agree with biochemical and physiological studies suggesting an association between ATP and ciliary activity.

Inhibition of Mucociliary Clearance of the Eustachian Tube by Leukotrienes C4 and D4

1995 ◽  
Vol 104 (3) ◽  
pp. 231-236 ◽  
Author(s):  
Tetsuya Ganbo ◽  
Tsukasa Nakajima ◽  
Ken-Ichi Hisamaisu ◽  
Hajime Inoue ◽  
Shin-Ichi Shimomura ◽  
...  
Keyword(s):  
Eustachian Tube ◽  
Mucociliary Clearance ◽  
Ciliary Activity ◽  
Leukotriene D4 ◽  
Auditory Brain Stem Response ◽  
Auditory Brain Stem ◽  
Brain Stem Response ◽  
Ear Effusion

The effect of leukotrienes C4 (LTC4) and D4 (LTD4) and prostaglandin E2 (PGE2) on mucociliary clearance of the eustachian tube was investigated in vitro and in vivo. Normal ciliated epithelium was obtained from the eustachian tube of guinea pigs and incubated separately with LTC4, LTD4, and PGE2 at concentrations of 10−8 mol/L and 10−6 mol/L. Ciliary activity was measured photoelectrically. Leukotriene D4 progressively inhibited ciliary activity, while PGE2 promoted it. Leukotriene C4 also induced ciliary inhibition. One milliliter each of 10−5 mol/L LTC4, LTD4, and PGE2 was directly injected into the tympanic bullae of chinchillas under anesthesia. The middle ears were examined by otomicroscopy, tympanometry, and auditory brain stem response over time. Clearance of middle ear effusion was delayed by LTC4 and LTD4, as compared with PGE2 and the control. These findings indicate that LTC4 and LTD4 inhibit mucociliary clearance of the eusiachian tube.

scholarly journals Epinephrine stimulation of anion secretion in the Calu-3 serous cell model

2014 ◽  
Vol 306 (10) ◽  
pp. L937-L946 ◽  
Author(s):  
Amiraj Banga ◽  
Stephanie Flaig ◽  
Shanta Lewis ◽  
Seth Winfree ◽  
Bonnie L. Blazer-Yost
Keyword(s):  
Adrenergic Receptor ◽  
Mucociliary Clearance ◽  
Cell Model ◽  
Transmembrane Protein ◽  
Intracellular Camp ◽  
Anion Secretion ◽  
Dual Channel ◽  
Submucosal Glands ◽  
Serous Cell ◽  
Well Differentiated

Calu-3 is a well-differentiated human bronchial cell line with the characteristics of the serous cells of airway submucosal glands. The submucosal glands play a major role in mucociliary clearance because they secrete electrolytes that facilitate airway hydration. Given the significance of both long- and short-term β-adrenergic receptor agonists in the treatment of respiratory diseases, it is important to determine the role of these receptors and their ligands in normal physiological function. The present studies were designed to characterize the effect of epinephrine, the naturally occurring β-adrenergic receptor agonist, on electrolyte transport of the airway serous cells. Interestingly, epinephrine stimulated two anion secretory channels, the cystic fibrosis transmembrane conductance regulator and a Ca2+-activated Cl− channel, with the characteristics of transmembrane protein 16A, thereby potentially altering mucociliary clearance via multiple channels. Consistent with the dual channel activation, epinephrine treatment resulted in increases in both intracellular cAMP and Ca2+. Furthermore, the present results extend previous reports indicating that the two anion channels are functionally linked.

Video image analysis of ciliary activity of human respiratory epithelium in culture

2003 ◽  
Author(s):  
E. Lamiot ◽  
J.-M. Zahm ◽  
D. Pierrot ◽  
M. Chevillard ◽  
J. Hinnrasky ◽  
...  
Keyword(s):  
Image Analysis ◽  
Respiratory Epithelium ◽  
Video Image ◽  
Ciliary Activity ◽  
Video Image Analysis

scholarly journals Transduction of Well-Differentiated Airway Epithelium by Recombinant Adeno-Associated Virus Is Limited by Vector Entry

1999 ◽  
Vol 73 (7) ◽  
pp. 6085-6088 ◽  
Author(s):  
Robert Bals ◽  
Weidong Xiao ◽  
Nianli Sang ◽  
Daniel J. Weiner ◽  
Rupalie L. Meegalla ◽  
...  
Keyword(s):  
Airway Epithelium ◽  
Respiratory Epithelium ◽  
Transduction Efficiency ◽  
Physical Barrier ◽  
Airway Epithelia ◽  
Adeno Associated Virus ◽  
Differentiated Cells ◽  
Undifferentiated Cells ◽  
Well Differentiated ◽  
Air Liquid Interface

ABSTRACT The limitations of adeno-associated virus (AAV)-mediated vectors for lung-directed gene transfer were investigated by using differentiated human respiratory epithelium in air-liquid interface cultures. Transduction efficiency was high in undifferentiated cells and was enhanced in well-differentiated cells after basolateral application of the vector or after apical application following disruption of tight junctions or pretreatment of the cultures with glycosidases. These results indicate that transduction of airway epithelia by AAV vectors is limited by entry and reinforce the importance of a physical barrier on the airway surface.

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