Novel Computed Tomography for Reconstructing Three-Dimensional Phase Contrast Images of Single Living Cells

2006 ◽  
Vol 18 (6) ◽  
pp. 827-830
Author(s):  
Yusuke Inoue ◽  
◽  
Ichirou Ishimaru ◽  
Toshiki Yasokawa ◽  
Katsumi Ishizaki ◽  
...  
Keyword(s):  
Refractive Index ◽  
Single Cells ◽  
Three Dimensional ◽  
High Refractive Index ◽  
Cross Sectional ◽  
3 Dimensional ◽  
Index Distribution ◽  
Single Living Cells ◽  
Cellular Components ◽  
Single Living

We proposed using spectroscopy-tomography of single cells as a diagnostic tool for improving early cancer detection and treatment. This technology potentially obtains the 3D distribution of cellular components at high spatial resolution. We used spectroscopy-tomography to generate and analyze a series of cross-sectional images of a borosilicate glass microsphere 10µm in diameter, easily determining the distribution of internal submicron defects in the microsphere. We also used this to estimate the 3-dimensional refractive index distribution in a single human breast cancer cell. The nucleus, which has a high refractive index, is clearly distinguishable.

scholarly journals Simultaneous quantification of multiple endogenous biothiols in single living cells by plasmonic Raman probes

2017 ◽  
Vol 8 (11) ◽  
pp. 7582-7587 ◽  
Author(s):  
Shan-Shan Li ◽  
Qi-Yuan Guan ◽  
Mengmeng Zheng ◽  
Yu-Qi Wang ◽  
Deju Ye ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Single Cells ◽  
Principal Component ◽  
Component Analysis ◽  
Living Cells ◽  
Simultaneous Quantification ◽  
Single Living Cells ◽  
Single Living

Three endogenous biothiols in single cells were simultaneously quantified by plasmonic Raman probes and quantitative principal component analysis (qPCA).

Template-Free Fabrication of Aligned Nanoarray for Quantifying the Nanosurface-Single Cells Interaction

2018 ◽  
Author(s):  
Biran Wang ◽  
Liming Wang ◽  
Shiren Wang
Keyword(s):  
Standard Deviation ◽  
High Reliability ◽  
Low Cost ◽  
Single Cells ◽  
Living Cells ◽  
Nanowire Arrays ◽  
Template Free ◽  
Sense And Respond ◽  
Single Living Cells ◽  
Single Living

In this paper, we for the first-time synthesized vertically aligned polyaniline (PANI) nanowire arrays on flat-end AFM tips via template-free solution methods. 4-Aminothiophenol was used for tailoring the nucleation size, chain propagation and orientation of the PANI nanowires. The microscopy characterization indicated that diameter was centered at a mean of 33.7 nm with a standard deviation of 6.5 nm, and length was centered at a mean of 50.3 nm with a standard deviation of 7.6 nm. PANI nanowire arrays are non-toxic, low-cost, and tunable, and thus PANI nanowire-grown tips could perfectly simulate different nanosurfaces. Via the force spectroscopy, we demonstrate the feasibility in quantifying the nanostructure-cell interactions at the single cell level in real time with high reliability and accuracy. This work will enable a new tool in precisely quantifying the interactions of single living cells and nanosurface, and thus opens a new door to understand how single living cells sense and respond to the specific nanostructures.

Three-Dimensional Anatomic Characterization of the Canine Laryngeal Abductor and Adductor Musculature

2000 ◽  
Vol 109 (5) ◽  
pp. 505-513 ◽  
Author(s):  
Corey W. Mineck ◽  
Roger Chan ◽  
Niro Tayama ◽  
Ingo R. Titze
Keyword(s):  
Vocal Fold ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Cross Sectional ◽  
3 Dimensional ◽  
Moment Arms ◽  
Intrinsic Laryngeal Muscle ◽  
Posterior Cricoarytenoid ◽  
Intrinsic Laryngeal Muscles

The biomechanics of vocal fold abduction and adduction during phonation, respiration, and airway protection are not completely understood. Specifically, the rotational and translational forces on the arytenoid cartilages that result from intrinsic laryngeal muscle contraction have not been fully described. Anatomic data on the lines of action and moment arms for the intrinsic laryngeal muscles are also lacking. This study was conducted to quantify the 3-dimensional orientations and the relative cross-sectional areas of the intrinsic abductor and adductor musculature of the canine larynx. Eight canine larynges were used to evaluate the 3 muscles primarily responsible for vocal fold abduction and adduction: the posterior cricoarytenoid, the lateral cricoarytenoid, and the interarytenoid muscles. Each muscle was exposed and divided into discrete fiber bundles whose coordinate positions were digitized in 3-dimensional space. The mass, length, relative cross-sectional area, and angle of orientation for each muscle bundle were obtained to allow for the calculations of average lines of action and moment arms for each muscle. This mapping of the canine laryngeal abductor and adductor musculature provides important anatomic data for use in laryngeal biomechanical modeling. These data may also be useful in surgical procedures such as arytenoid adduction.

Three-Dimensional Analysis of Scapular Kinematics During Arm Elevation in Baseball Players With Scapular Dyskinesis: Comparison of Dominant and Nondominant Arms

2020 ◽  
Vol 29 (1) ◽  
pp. 93-101 ◽  
Author(s):  
Jin-Young Park ◽  
Junhyun Kim ◽  
Beom Ho Seo ◽  
Ho Dong Yu ◽  
Ju Hyun Sim ◽  
...  
Keyword(s):  
Pathological Condition ◽  
Three Dimensional ◽  
Diagnostic Study ◽  
Scapular Dyskinesis ◽  
Level Of Evidence ◽  
Cross Sectional ◽  
Baseball Players ◽  
3 Dimensional ◽  
Scapular Kinematics ◽  
Arm Elevation

Background: The knowledge of 3-dimensional scapular kinematics is essential for understanding the pathological lesions of the shoulder and elbow in throwing athletes. Many studies about alterations of the resting scapular position, dynamic scapular motion, or scapular dyskinesis (SD) have been conducted, yet none of them have identified a consistent pattern of altered scapular kinematics in throwing athletes. Hypothesis/Purpose: To analyze the 3-dimensional scapular kinematics of dominant and nondominant arms in baseball players with a pathological condition of the shoulder or elbow. Study Design: Cross-sectional study. Methods: Bilateral scapular positions, consisting of upward rotation (UR), superior translation (ST), internal rotation (IR), protraction (PRO), and anterior tilting (AT) with an arm at rest and at 150° forward elevation, were measured among 319 baseball players with SD using 3-dimensional computed tomography. Angular values of scapula were compared between dominant and nondominant arms with statistical analysis. Level of Evidence: Level III, diagnostic study. Results: The scapular position of dominant arms showed significantly more AT, less ST at rest and more UR and IR and less ST, PRO, and AT at 150° full forward elevation compared with the nondominant arms. The magnitude of mean change of UR, IR, PRO, and AT during arm elevation increased significantly between the paired arms (P value: UR, ST, PRO, and AT: <.001 and IR: .001). Conclusion: When compared with the nondominant arms, UR, AT, and PRO with the arm at 150° forward elevation of dominant symptomatic arms in baseball players tilted toward positive compensation, whereas IR altered toward negative decompensation. In addition, the angular increment of the scapula increased significantly in dominant symptomatic arms compared with the nondominant arms.

Confocal microscopy of single living cells

1995 ◽  
Vol 53 ◽  
pp. 792-793
Author(s):  
John J. Lemasters
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Single Cells ◽  
Laser Scanning Confocal Microscopy ◽  
Living Cells ◽  
Repeated Measurements ◽  
Scanning Electron Micrographs ◽  
Scanning Confocal Microscopy ◽  
Single Living Cells ◽  
Single Living

The advent of laser scanning confocal microscopy solves the dilemma of studying thick specimens with optical microscopy by creating optical slices less than 1 μm in thickness. Increasingly, confocal microscopy is an essential analytical tool for studying the structure and physiology of living cells. Because confocal microscopy collects light from only a fraction of the specimen volume, greater illumination is required. Consequently, photodamage and photobleaching are greater considerations, especially for study for living cells where repeated measurements over time are desired. To minimize photodamage, laser intensity should be attenuated by 100-1000 fold, photomultiplier circuits should be operated at highest sensitivity, and stable fluorophores should be used. When these conditions are met, literally hundreds of high resolution confocal images can be obtained from single cells loaded with parameter sensitive fluorophores.The number of parameter-specific fluorophores useful for observing single living cells by confocal microscopy is large and increasing. By labeling with calcein and collecting serial images, the volume, shape and surface topography of single living cells are reconstructed with results rivaling scanning electron micrographs.

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