Template-Free Fabrication of Aligned Nanoarray for Quantifying the Nanosurface-Single Cells Interaction

2018 ◽  
Author(s):  
Biran Wang ◽  
Liming Wang ◽  
Shiren Wang
Keyword(s):  
Standard Deviation ◽  
High Reliability ◽  
Low Cost ◽  
Single Cells ◽  
Living Cells ◽  
Nanowire Arrays ◽  
Template Free ◽  
Sense And Respond ◽  
Single Living Cells ◽  
Single Living

In this paper, we for the first-time synthesized vertically aligned polyaniline (PANI) nanowire arrays on flat-end AFM tips via template-free solution methods. 4-Aminothiophenol was used for tailoring the nucleation size, chain propagation and orientation of the PANI nanowires. The microscopy characterization indicated that diameter was centered at a mean of 33.7 nm with a standard deviation of 6.5 nm, and length was centered at a mean of 50.3 nm with a standard deviation of 7.6 nm. PANI nanowire arrays are non-toxic, low-cost, and tunable, and thus PANI nanowire-grown tips could perfectly simulate different nanosurfaces. Via the force spectroscopy, we demonstrate the feasibility in quantifying the nanostructure-cell interactions at the single cell level in real time with high reliability and accuracy. This work will enable a new tool in precisely quantifying the interactions of single living cells and nanosurface, and thus opens a new door to understand how single living cells sense and respond to the specific nanostructures.

scholarly journals Simultaneous quantification of multiple endogenous biothiols in single living cells by plasmonic Raman probes

2017 ◽  
Vol 8 (11) ◽  
pp. 7582-7587 ◽  
Author(s):  
Shan-Shan Li ◽  
Qi-Yuan Guan ◽  
Mengmeng Zheng ◽  
Yu-Qi Wang ◽  
Deju Ye ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Single Cells ◽  
Principal Component ◽  
Component Analysis ◽  
Living Cells ◽  
Simultaneous Quantification ◽  
Single Living Cells ◽  
Single Living

Three endogenous biothiols in single cells were simultaneously quantified by plasmonic Raman probes and quantitative principal component analysis (qPCA).

Confocal microscopy of single living cells

1995 ◽  
Vol 53 ◽  
pp. 792-793
Author(s):  
John J. Lemasters
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Single Cells ◽  
Laser Scanning Confocal Microscopy ◽  
Living Cells ◽  
Repeated Measurements ◽  
Scanning Electron Micrographs ◽  
Scanning Confocal Microscopy ◽  
Single Living Cells ◽  
Single Living

The advent of laser scanning confocal microscopy solves the dilemma of studying thick specimens with optical microscopy by creating optical slices less than 1 μm in thickness. Increasingly, confocal microscopy is an essential analytical tool for studying the structure and physiology of living cells. Because confocal microscopy collects light from only a fraction of the specimen volume, greater illumination is required. Consequently, photodamage and photobleaching are greater considerations, especially for study for living cells where repeated measurements over time are desired. To minimize photodamage, laser intensity should be attenuated by 100-1000 fold, photomultiplier circuits should be operated at highest sensitivity, and stable fluorophores should be used. When these conditions are met, literally hundreds of high resolution confocal images can be obtained from single cells loaded with parameter sensitive fluorophores.The number of parameter-specific fluorophores useful for observing single living cells by confocal microscopy is large and increasing. By labeling with calcein and collecting serial images, the volume, shape and surface topography of single living cells are reconstructed with results rivaling scanning electron micrographs.

scholarly journals Optofluidics for handling and analysis of single living cells

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Gerardo Perozziello ◽  
Patrizio Candeloro ◽  
Maria Laura Coluccio ◽  
Enzo Di Fabrizio
Keyword(s):  
Analytical Chemistry ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Photonic Devices ◽  
Living Cells ◽  
Optical Elements ◽  
Integrated Optical ◽  
Single Living Cells ◽  
Integrated Optical Sensor ◽  
Single Living

AbstractOptofluidics is a field with important applications in areas such as biotechnology, chemical synthesis and analytical chemistry. Optofluidic devices combine optical elements into microfluidic devices in ways that increase portability and sensitivity of analysis for diagnostic or screening purposes .In fact in these devices fluids give fine adaptability, mobility and accessibility to nanoscale photonic devices which otherwise could not be realized using conventional devices. This review describes several cases inwhich optical or microfluidic approaches are used to trap single cells in proximity of integrated optical sensor for being analysed.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

Probing nucleus-enriched proteins in single living cells via subcellular-resolved plasmonic immunosandwich assay

The Analyst ◽  
2021 ◽  
Author(s):  
Jia Liu ◽  
Dan Xie ◽  
Zhen Liu
Keyword(s):  
Living Cells ◽  
Nuclear Proteins ◽  
Biological Functions ◽  
Abnormal Expression ◽  
Single Living Cells ◽  
Single Living ◽  
In Cells

Nuclear proteins are crucial in cells and are greatly linked to various biological functions. Abnormal expression of nuclear proteins is associated with many diseases ranging from inflammation to cancer. However,...

scholarly journals Measurement of Separase Proteolytic Activity in Single Living Cells by a Fluorogenic Flow Cytometry Assay

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0133769 ◽  
Author(s):  
Wiltrud Haaß ◽  
Helga Kleiner ◽  
Martin C. Müller ◽  
Wolf-Karsten Hofmann ◽  
Alice Fabarius ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Proteolytic Activity ◽  
Living Cells ◽  
Flow Cytometry Assay ◽  
Single Living Cells ◽  
Single Living
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