scholarly journals Simultaneous quantification of multiple endogenous biothiols in single living cells by plasmonic Raman probes

2017 ◽  
Vol 8 (11) ◽  
pp. 7582-7587 ◽  
Author(s):  
Shan-Shan Li ◽  
Qi-Yuan Guan ◽  
Mengmeng Zheng ◽  
Yu-Qi Wang ◽  
Deju Ye ◽  
...  
Keyword(s):  
Principal Component Analysis ◽  
Single Cells ◽  
Principal Component ◽  
Component Analysis ◽  
Living Cells ◽  
Simultaneous Quantification ◽  
Single Living Cells ◽  
Single Living

Three endogenous biothiols in single cells were simultaneously quantified by plasmonic Raman probes and quantitative principal component analysis (qPCA).

scholarly journals Demagnetization Analysis in Excel (DAIE). An open source workbook in Excel for viewing and analyzing demagnetization data from paleomagnetic discrete samples and u-channels

2013 ◽  
Vol 56 (1) ◽  
Author(s):  
Leonardo Sagnotti
Keyword(s):  
Principal Component Analysis ◽  
Open Source ◽  
Single Cells ◽  
Principal Component ◽  
Component Analysis ◽  
Modular Structure ◽  
Microsoft Excel ◽  
Excel File

<p>The Demagnetization Analysis in Excel (DAIE) software is a single Microsoft Excel file designed for viewing and analyzing stepwise demagnetization data of both discrete and u-channel samples in paleomagnetic studies. DAIE is an Excel workbook and has an open modular structure organized in 10 worksheets. It is designed for an easy use and an interactive operability; all the commands and choices can be entered by sliding menus associated to single cells. The standard demagnetization diagrams and various parameters of common use are shown on the same worksheet including selectable parameters and user's choices. The remanence characteristic components may be computed by principal component analysis (PCA) on a selected interval of demagnetization steps. Saving of the PCA data can be done both sample by sample, or in automatic by applying the selected choices to all the samples included in the file. The whole workbook is free both for use and editing and it is available for download on a dedicated website.</p>

Template-Free Fabrication of Aligned Nanoarray for Quantifying the Nanosurface-Single Cells Interaction

2018 ◽  
Author(s):  
Biran Wang ◽  
Liming Wang ◽  
Shiren Wang
Keyword(s):  
Standard Deviation ◽  
High Reliability ◽  
Low Cost ◽  
Single Cells ◽  
Living Cells ◽  
Nanowire Arrays ◽  
Template Free ◽  
Sense And Respond ◽  
Single Living Cells ◽  
Single Living

In this paper, we for the first-time synthesized vertically aligned polyaniline (PANI) nanowire arrays on flat-end AFM tips via template-free solution methods. 4-Aminothiophenol was used for tailoring the nucleation size, chain propagation and orientation of the PANI nanowires. The microscopy characterization indicated that diameter was centered at a mean of 33.7 nm with a standard deviation of 6.5 nm, and length was centered at a mean of 50.3 nm with a standard deviation of 7.6 nm. PANI nanowire arrays are non-toxic, low-cost, and tunable, and thus PANI nanowire-grown tips could perfectly simulate different nanosurfaces. Via the force spectroscopy, we demonstrate the feasibility in quantifying the nanostructure-cell interactions at the single cell level in real time with high reliability and accuracy. This work will enable a new tool in precisely quantifying the interactions of single living cells and nanosurface, and thus opens a new door to understand how single living cells sense and respond to the specific nanostructures.

Confocal microscopy of single living cells

1995 ◽  
Vol 53 ◽  
pp. 792-793
Author(s):  
John J. Lemasters
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Single Cells ◽  
Laser Scanning Confocal Microscopy ◽  
Living Cells ◽  
Repeated Measurements ◽  
Scanning Electron Micrographs ◽  
Scanning Confocal Microscopy ◽  
Single Living Cells ◽  
Single Living

The advent of laser scanning confocal microscopy solves the dilemma of studying thick specimens with optical microscopy by creating optical slices less than 1 μm in thickness. Increasingly, confocal microscopy is an essential analytical tool for studying the structure and physiology of living cells. Because confocal microscopy collects light from only a fraction of the specimen volume, greater illumination is required. Consequently, photodamage and photobleaching are greater considerations, especially for study for living cells where repeated measurements over time are desired. To minimize photodamage, laser intensity should be attenuated by 100-1000 fold, photomultiplier circuits should be operated at highest sensitivity, and stable fluorophores should be used. When these conditions are met, literally hundreds of high resolution confocal images can be obtained from single cells loaded with parameter sensitive fluorophores.The number of parameter-specific fluorophores useful for observing single living cells by confocal microscopy is large and increasing. By labeling with calcein and collecting serial images, the volume, shape and surface topography of single living cells are reconstructed with results rivaling scanning electron micrographs.

scholarly journals Optofluidics for handling and analysis of single living cells

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Gerardo Perozziello ◽  
Patrizio Candeloro ◽  
Maria Laura Coluccio ◽  
Enzo Di Fabrizio
Keyword(s):  
Analytical Chemistry ◽  
Single Cells ◽  
Microfluidic Devices ◽  
Photonic Devices ◽  
Living Cells ◽  
Optical Elements ◽  
Integrated Optical ◽  
Single Living Cells ◽  
Integrated Optical Sensor ◽  
Single Living

AbstractOptofluidics is a field with important applications in areas such as biotechnology, chemical synthesis and analytical chemistry. Optofluidic devices combine optical elements into microfluidic devices in ways that increase portability and sensitivity of analysis for diagnostic or screening purposes .In fact in these devices fluids give fine adaptability, mobility and accessibility to nanoscale photonic devices which otherwise could not be realized using conventional devices. This review describes several cases inwhich optical or microfluidic approaches are used to trap single cells in proximity of integrated optical sensor for being analysed.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

A German version of the Intermittent Claudication Questionnaire (ICQ): cultural adaptation and validation

VASA ◽  
2012 ◽  
Vol 41 (5) ◽  
pp. 333-342 ◽  
Author(s):  
Kirchberger ◽  
Finger ◽  
Müller-Bühl
Keyword(s):  
Principal Component Analysis ◽  
Intermittent Claudication ◽  
Completion Time ◽  
Short Form ◽  
Principal Component ◽  
Component Analysis ◽  
German Version ◽  
Average Completion Time ◽  
Sf 36 ◽  
Related Quality

Background: The Intermittent Claudication Questionnaire (ICQ) is a short questionnaire for the assessment of health-related quality of life (HRQOL) in patients with intermittent claudication (IC). The objective of this study was to translate the ICQ into German and to investigate the psychometric properties of the German ICQ version in patients with IC. Patients and methods: The original English version was translated using a forward-backward method. The resulting German version was reviewed by the author of the original version and an experienced clinician. Finally, it was tested for clarity with 5 German patients with IC. A sample of 81 patients were administered the German ICQ. The sample consisted of 58.0 % male patients with a median age of 71 years and a median IC duration of 36 months. Test of feasibility included completeness of questionnaires, completion time, and ratings of clarity, length and relevance. Reliability was assessed through a retest in 13 patients at 14 days, and analysis of Cronbach’s alpha for internal consistency. Construct validity was investigated using principal component analysis. Concurrent validity was assessed by correlating the ICQ scores with the Short Form 36 Health Survey (SF-36) as well as clinical measures. Results: The ICQ was completely filled in by 73 subjects (90.1 %) with an average completion time of 6.3 minutes. Cronbach’s alpha coefficient reached 0.75. Intra-class correlation for test-retest reliability was r = 0.88. Principal component analysis resulted in a 3 factor solution. The first factor explained 51.5 of the total variation and all items had loadings of at least 0.65 on it. The ICQ was significantly associated with the SF-36 and treadmill-walking distances whereas no association was found for resting ABPI. Conclusions: The German version of the ICQ demonstrated good feasibility, satisfactory reliability and good validity. Responsiveness should be investigated in further validation studies.

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