scholarly journals Comparative analysis of the Accelerated Aged seed transcriptome profiles of maize CSSLs (I178 and X178)

2019 ◽  
Author(s):  
Li Li ◽  
Feng Wang ◽  
Xuhui Li ◽  
Yixuan Peng ◽  
Hongwei Zhang ◽  
...  
Keyword(s):  
Seed Quality ◽  
Seed Longevity ◽  
Accelerated Ageing ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Carbohydrate Derivative ◽  
Significant Difference ◽  
Seed Ageing ◽  
Aged Seed ◽  
Segment Substitution

AbstractSeed longevity is one of the most essential characters of seed quality. Two Chromosome segment substitution lines (CSSL) I178 and X178 with significant difference on seed longevity were subjected to transcriptome sequencing before (0d-AA) and after five days of accelerated ageing (5d-AA) treatments. Compared to the non-accelerated ageing treatment (0d-AA), 286 and 220 differential expressed genes (DEGs) were identified in I178 and X178, respectively Among those, 98 DEGs were detected in both I178 and X178 after 5d-AA, Enriched GO terms included cellular components of cell part, intracellular part, organelle and membrane etc., including carbohydrate derivative catabolic process, carbohydrate synthesis, sugar isomerase (SIS) family protein etc. Transcriptome analysis of I178 and X178 showed that Alternative splicing (AS) occurs in 63.6% of the expressed genes in all samples. Only 381 genes specifically occurred AS in I178 and X178 after 5d-AA, mostly enriched in nucleotide and nucleoside binding. Combined with the reported QTL mapping result, the DEG and the AS information, 13 DEGs in the mapping intervals and 7 AS-DEGs were potential candidates may directly or indirectly associated to seed ageing.

scholarly journals Genetic Dissection of Seed Dormancy using Chromosome Segment Substitution Lines in Rice (Oryza sativa L.)

2020 ◽  
Vol 21 (4) ◽  
pp. 1344 ◽  
Author(s):  
Shaowen Yuan ◽  
Yuntong Wang ◽  
Chaopu Zhang ◽  
Hanzi He ◽  
Sibin Yu
Keyword(s):  
Seed Dormancy ◽  
Genetic Architecture ◽  
Oryza Sativa L ◽  
Expression Profiles ◽  
Chromosome Segment ◽  
Isogenic Line ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Segment Substitution ◽  
Seed Dormancy And Germination

Timing of germination determines whether a new plant life cycle can be initiated; therefore, appropriate dormancy and rapid germination under diverse environmental conditions are the most important features for a seed. However, the genetic architecture of seed dormancy and germination behavior remains largely elusive. In the present study, a linkage analysis for seed dormancy and germination behavior was conducted using a set of 146 chromosome segment substitution lines (CSSLs), of which each carries a single or a few chromosomal segments of Nipponbare (NIP) in the background of Zhenshan 97 (ZS97). A total of 36 quantitative trait loci (QTLs) for six germination parameters were identified. Among them, qDOM3.1 was validated as a major QTL for seed dormancy in a segregation population derived from the qDOM3.1 near-isogenic line, and further delimited into a genomic region of 90 kb on chromosome 3. Based on genetic analysis and gene expression profiles, the candidate genes were restricted to eight genes, of which four were responsive to the addition of abscisic acid (ABA). Among them, LOC_Os03g01540 was involved in the ABA signaling pathway to regulate seed dormancy. The results will facilitate cloning the major QTLs and understanding the genetic architecture for seed dormancy and germination in rice and other crops.

scholarly journals Discerning combining ability loci for divergent environments using chromosome segment substitution lines (CSSLs) in pearl millet

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0218916 ◽  
Author(s):  
Ramana Kumari Basava ◽  
Charles Thomas Hash ◽  
Mahesh D. Mahendrakar ◽  
Kavi Kishor P. B. ◽  
C. Tara Satyavathi ◽  
...  
Keyword(s):  
Pearl Millet ◽  
Combining Ability ◽  
Chromosome Segment ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines ◽  
Segment Substitution
Sign in / Sign up

Export Citation Format

Share Document