scholarly journals Discovery of fifteen new geroprotective plant extracts and identification of cellular processes they affect to prolong the chronological lifespan of budding yeast

Oncotarget ◽  
2020 ◽  
Vol 11 (23) ◽  
pp. 2182-2203
Author(s):  
Pamela Dakik ◽  
Monica Enith Lozano Rodriguez ◽  
Jennifer Anne Baratang Junio ◽  
Darya Mitrofanova ◽  
Younes Medkour ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Budding Yeast ◽  
Chronological Lifespan ◽  
Cellular Processes

scholarly journals Construction and use of a microfluidic dissection platform for long-term imaging of cellular processes in budding yeast

2013 ◽  
Vol 8 (6) ◽  
pp. 1019-1027 ◽  
Author(s):  
Daphne H E W Huberts ◽  
Sung Sik Lee ◽  
Javier González ◽  
Georges E Janssens ◽  
Ima Avalos Vizcarra ◽  
...  
Keyword(s):  
Budding Yeast ◽  
Cellular Processes

scholarly journals DNA Replication Stress Is a Determinant of Chronological Lifespan in Budding Yeast

PLoS ONE ◽  
2007 ◽  
Vol 2 (8) ◽  
pp. e748 ◽  
Author(s):  
Martin Weinberger ◽  
Li Feng ◽  
Anita Paul ◽  
Daniel L. Smith ◽  
Robert D. Hontz ◽  
...  
Keyword(s):  
Dna Replication ◽  
Budding Yeast ◽  
Replication Stress ◽  
Chronological Lifespan ◽  
Dna Replication Stress

scholarly journals Spontaneous mutations in CYC8 and MIG1 suppress the short chronological lifespan of budding yeast lacking SNF1/AMPK

2018 ◽  
Vol 5 (5) ◽  
pp. 233-248 ◽  
Author(s):  
Nazif Maqani ◽  
Ryan D. Fine ◽  
Mehreen Shahid ◽  
Mingguang Li ◽  
Elisa Enriquez-Hesles ◽  
...  
Keyword(s):  
Budding Yeast ◽  
Spontaneous Mutations ◽  
Chronological Lifespan

scholarly journals Identification of Tropical Plant Extracts That Extend Yeast Chronological Life Span

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2718
Author(s):  
Mandy Mun Yee Kwong ◽  
Jee Whu Lee ◽  
Mohammed Razip Samian ◽  
Habibah A. Wahab ◽  
Nobumoto Watanabe ◽  
...  
Keyword(s):  
Life Span ◽  
Plant Extracts ◽  
Stress Resistance ◽  
Manihot Esculenta ◽  
Dependent Manner ◽  
Leaf Extracts ◽  
Cellular Processes ◽  
Tropical Plant ◽  
Chronological Life Span ◽  
Dose Dependent

Certain plant extracts (PEs) contain bioactive compounds that have antioxidant and lifespan-extending activities on organisms. These PEs play different roles in cellular processes, such as enhancing stress resistance and modulating longevity-defined signaling pathways that contribute to longevity. Here, we report the discovery of PEs that extended chronological life span (CLS) in budding yeast from a screen of 222 PEs. We identified two PEs, the leaf extracts of Manihot esculenta and Wodyetia bifurcata that extended CLS in a dose-dependent manner. The CLS-extending PEs also conferred oxidative stress tolerance, suggesting that these PEs might extend yeast CLS through the upregulation of stress response pathways.

scholarly journals Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation

2006 ◽  
Vol 174 (5) ◽  
pp. 653-663 ◽  
Author(s):  
Ben Montpetit ◽  
Tony R. Hazbun ◽  
Stanley Fields ◽  
Philip Hieter
Keyword(s):  
Protein Function ◽  
Budding Yeast ◽  
Chromosome Instability ◽  
Critical Role ◽  
Mitotic Checkpoint ◽  
Kinetochore Protein ◽  
Cellular Processes ◽  
Potential Link ◽  
Important Regulator ◽  
Spindle Elongation

Posttranslational modification by the ubiquitin-like protein SUMO (small ubiquitin-like modifier) is emerging as an important regulator in many cellular processes, including genome integrity. In this study, we show that the kinetochore proteins Ndc10, Bir1, Ndc80, and Cep3, which mediate the attachment of chromosomes to spindle microtubules, are sumoylated substrates in budding yeast. Furthermore, we show that Ndc10, Bir1, and Cep3 but not Ndc80 are desumoylated upon exposure to nocodazole, highlighting the possibility of distinct roles for sumoylation in modulating kinetochore protein function and of a potential link between the sumoylation of kinetochore proteins and mitotic checkpoint function. We find that lysine to arginine mutations that eliminate the sumoylation of Ndc10 cause chromosome instability, mislocalization of Ndc10 from the mitotic spindle, abnormal anaphase spindles, and a loss of Bir1 sumoylation. These data suggest that sumoylation of Ndc10 and other kinetochore proteins play a critical role during the mitotic process.

scholarly journals A rapid, high-throughput method for determining chronological lifespan in budding yeast

2018 ◽  
Vol 5 (4) ◽  
pp. 106 ◽  
Author(s):  
Zachery R. Belak ◽  
Troy Harkness ◽  
Christopher H. Eskiw
Keyword(s):  
High Throughput ◽  
Budding Yeast ◽  
Chronological Lifespan ◽  
High Throughput Method

scholarly journals Single-cell phenomics in budding yeast

2015 ◽  
Vol 26 (22) ◽  
pp. 3920-3925 ◽  
Author(s):  
Yoshikazu Ohya ◽  
Yoshitaka Kimori ◽  
Hiroki Okada ◽  
Shinsuke Ohnuki
Keyword(s):  
Cell Imaging ◽  
Budding Yeast ◽  
Model Organism ◽  
Eukaryotic Cells ◽  
High Dimensional ◽  
Biological Knowledge ◽  
Yeast Saccharomyces Cerevisiae ◽  
Cellular Processes ◽  
Designed Experiment ◽  
High Throughput Phenotyping

The demand for phenomics, a high-dimensional and high-throughput phenotyping method, has been increasing in many fields of biology. The budding yeast Saccharomyces cerevisiae, a unicellular model organism, provides an invaluable system for dissecting complex cellular processes using high-resolution phenotyping. Moreover, the addition of spatial and temporal attributes to subcellular structures based on microscopic images has rendered this cell phenotyping system more reliable and amenable to analysis. A well-designed experiment followed by appropriate multivariate analysis can yield a wealth of biological knowledge. Here we review recent advances in cell imaging and illustrate their broad applicability to eukaryotic cells by showing how these techniques have advanced our understanding of budding yeast.

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