scholarly journals Caloric Restriction-Induced Extension of Chronological Lifespan Requires Intact Respiration in Budding Yeast

2017 ◽  
Vol 40 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Young-Yon Kwon ◽  
Sung-Keun Lee ◽  
Cheol-Koo Lee
Keyword(s):  
Caloric Restriction ◽  
Budding Yeast ◽  
Chronological Lifespan

COCOA (Theobroma cacao) POLYPHENOL-RICH EXTRACT INCREASES THE CHRONOLOGICAL LIFESPAN OF Saccharomyces cerevisiae

2016 ◽  
pp. 1-5
Author(s):  
I. BAIGES ◽  
L. AROLA
Keyword(s):  
Stationary Phase ◽  
Caloric Restriction ◽  
High Throughput ◽  
Animal Studies ◽  
Model Organism ◽  
Dependent Manner ◽  
Aging Effects ◽  
Screening Assay ◽  
Chronological Lifespan ◽  
Synthetic Complete

Background:Saccharomyces cerevisiae is a model organism with conserved aging pathways. Yeast chronological lifespan experiments mimic the processes involved in human non-dividing tissues, such as the nervous system or skeletal muscle, and can speed up the search for biomolecules with potential anti-aging effects before proceeding to animal studies. Objective: To test the effectiveness of a cocoa polyphenol-rich extract (CPE) in expanding the S. cerevisiae chronological lifespan in two conditions: in the stationary phase reached after glucose depletion and under severe caloric restriction. Measurements: Using a high-throughput method, wild-type S. cerevisiae and its mitochondrial manganese-dependent superoxide dismutase null mutant (sod2Δ) were cultured in synthetic complete dextrose medium. After 2 days, 0, 5 and 20 mg/ml of CPE were added, and viability was measured throughout the stationary phase. The effects of the major components of CPE were also evaluated. To determine yeast lifespan under severe caloric restriction conditions, cultures were washed with water 24 h after the addition of 0 and 20 mg/ml of CPE, and viability was followed over time. Results: CPE increased the chronological lifespan of S. cerevisiae during the stationary phase in a dose-dependent manner. A similar increase was also observed in (sod2Δ). None of the major CPE components (theobromine, caffeine, maltodextrin, (-)-epicatechin, (+)-catechin and procyanidin B2) was able to increase the yeast lifespan. CPE further increased the yeast lifespan under severe caloric restriction. Conclusion: CPE increases the chronological lifespan of S. cerevisiae through a SOD2-independent mechanism. The extract also extends yeast lifespan under severe caloric restriction conditions. The high-throughput assay used makes it possible to simply and rapidly test the efficacy of a large number of compounds on yeast aging, requiring only small amounts, and is thus a convenient screening assay to accelerate the search for biomolecules with potential anti-aging effects.

scholarly journals DNA Replication Stress Is a Determinant of Chronological Lifespan in Budding Yeast

PLoS ONE ◽  
2007 ◽  
Vol 2 (8) ◽  
pp. e748 ◽  
Author(s):  
Martin Weinberger ◽  
Li Feng ◽  
Anita Paul ◽  
Daniel L. Smith ◽  
Robert D. Hontz ◽  
...  
Keyword(s):  
Dna Replication ◽  
Budding Yeast ◽  
Replication Stress ◽  
Chronological Lifespan ◽  
Dna Replication Stress

scholarly journals Spontaneous mutations in CYC8 and MIG1 suppress the short chronological lifespan of budding yeast lacking SNF1/AMPK

2018 ◽  
Vol 5 (5) ◽  
pp. 233-248 ◽  
Author(s):  
Nazif Maqani ◽  
Ryan D. Fine ◽  
Mehreen Shahid ◽  
Mingguang Li ◽  
Elisa Enriquez-Hesles ◽  
...  
Keyword(s):  
Budding Yeast ◽  
Spontaneous Mutations ◽  
Chronological Lifespan

scholarly journals Mechanisms that Link Chronological Aging to Cellular Quiescence in Budding Yeast

2020 ◽  
Vol 21 (13) ◽  
pp. 4717
Author(s):  
Karamat Mohammad ◽  
Jennifer Anne Baratang Junio ◽  
Tala Tafakori ◽  
Emmanuel Orfanos ◽  
Vladimir I. Titorenko
Keyword(s):  
Caloric Restriction ◽  
Budding Yeast ◽  
Yeast Cells ◽  
Yeast Culture ◽  
Chronological Aging ◽  
Quiescent Cells ◽  
Cellular Events ◽  
Age Related ◽  
Calorie Diet ◽  
Cellular Quiescence

After Saccharomyces cerevisiae cells cultured in a medium with glucose consume glucose, the sub-populations of quiescent and non-quiescent cells develop in the budding yeast culture. An age-related chronology of quiescent and non-quiescent yeast cells within this culture is discussed here. We also describe various hallmarks of quiescent and non-quiescent yeast cells. A complex aging-associated program underlies cellular quiescence in budding yeast. This quiescence program includes a cascade of consecutive cellular events orchestrated by an intricate signaling network. We examine here how caloric restriction, a low-calorie diet that extends lifespan and healthspan in yeast and other eukaryotes, influences the cellular quiescence program in S. cerevisiae. One of the main objectives of this review is to stimulate an exploration of the mechanisms that link cellular quiescence to chronological aging of budding yeast. Yeast chronological aging is defined by the length of time during which a yeast cell remains viable after its growth and division are arrested, and it becomes quiescent. We propose a hypothesis on how caloric restriction can slow chronological aging of S. cerevisiae by altering the chronology and properties of quiescent cells. Our hypothesis posits that caloric restriction delays yeast chronological aging by targeting four different processes within quiescent cells.

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