scholarly journals Mitochondrial oxidation in the internal organs of rats with cranial injury and diabetes mellitus

2013 ◽  
Vol 94 (5) ◽  
pp. 621-627
Author(s):  
V M Meretskyy
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Succinate Dehydrogenase ◽  
Cytochrome Oxidase ◽  
Experimental Diabetes ◽  
Oxidative Modification ◽  
Experimental Diabetes Mellitus ◽  
Mitochondrial Oxidation ◽  
Potassium Atpase ◽  
Cranial Injury

Aim. To study the relation between the processes of mitochondrial oxidation and the intensity of lipid peroxidation, pulmonary and cardiac proteins oxidative modification at cranial injury, diabetes mellitus and at both. Methods. Experiments were carried out on 100 male white rats distributed to the following groups: the first group (n=10) - control group consisted of 10 intact animals, the second group (n=40) - rats with simulated cranial injury, the third group (n=10) - rats with experimental diabetes mellitus, the fourth group (n=40) - rats with simulated cranial injury and experimental diabetes mellitus. Experimental diabetes was induced by a single intraperitoneal injection of streptozotocin solution. Animals were withdrawn from the experiment at 3, 24 hours, 5 and 14 days after the cranial injury. Cardiac and pulmonary energy supply was estimated by the activity of succinate dehydrogenase, cytochrome oxidase and hydrogen potassium ATPase, as well as by the level of adenine nucleotides. Intensity of free-radical protein and lipid peroxidation was assessed by measuring the levels of neutral and base aldehyde and ketone derivatives and active products reacting with thiobarbituric acid. Results. Decrease of succinate dehydrogenase, cytochrome oxidase activity, a significant increase of hydrogen potassium ATPase activity in heart and lungs mitochondria was observed in rats with cranial injury, diabetes mellitus and especially with both conditions. Adenosine triphosphate tissue reserves have significantly decreased, while adenosine diphosphate and monophosphate levels increased, lipid peroxidation and protein oxidation processes activated. Conclusion. In rats with cranial injury associated with diabetes mellitus, lipid and protein peroxidation intensity parameters, the intensity of oxidative stress and the levels of macroergic substanses were significantly worse compared to the same parameters in the injured animals with normal blood glucose level.

scholarly journals Influence of thePENTAPHYLLOIDES FRUTICOSA l.lInIment on tba-actIve products and antIoxIdant status In wound InjurIes assocIated wIth experImental dIabetes mellItus

2016 ◽  
Vol 1 (5) ◽  
pp. 126-128
Author(s):  
Бальхаев ◽  
Mikhail Balkhaev ◽  
Гармаев ◽  
Bato-Tsyren Garmaev ◽  
Хитрихеев ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Experimental Diabetes ◽  
White Male ◽  
Control Group ◽  
Experimental Diabetes Mellitus ◽  
Male Wistar Rats ◽  
Pentaphylloides Fruticosa ◽  
Experimental Group

The aim of the work was to evaluate the influence of the liniment from Pentaphylloides fruticosa L. on lipid peroxidation in rats with experimental diabetes mellitus accompanied by wound process.The liniment was developed with the use of NanoDeBee30 device. Experiments were carried out on white male Wistar rats. Experimental diabetes mellitus was simulated by a single intraperitoneal introduction of alloxan in a dose of 100mg/kg. The skin injury was simulated by inflicting planar wounds 2.0×2.0cm in size. 5mg of the 5%-liniment from Pentaphylloides fruticosa was put on the wounds of the first experimental group once a day for 14 days. The sec-ond experimental group was treated by the preparation of comparison – balsamic liniment. The wounds of the control group were treated with glycerin-lanolin ointment. On the 7th, 14th and 21st day the concentration of TBA-active products in the blood serum was determined to estimate the intensity of lipid peroxidation processes; the contents of reduced glutathione and catalase activity were determined to evaluate the antioxidant status. The results of the experiments verify the marked curative effect of the liniment from Pentaphylloides fruticosa L. on the healing of the wounds on the background of experimental diabetes mellitus in rats

scholarly journals The features of neuroendocrine changes in cranial injury associated with diabetes mellitus

2013 ◽  
Vol 94 (4) ◽  
pp. 560-565
Author(s):  
V M Meretskyy ◽  
M M Korda
Keyword(s):  
Diabetes Mellitus ◽  
Nervous System ◽  
Autonomic Nervous System ◽  
Data Analysis ◽  
Serum Concentration ◽  
Experimental Diabetes ◽  
Experimental Diabetes Mellitus ◽  
Autonomic Nervous ◽  
White Rats ◽  
Cranial Injury

Aim. To study the features of neuroendocrine regulation in cranial injury associated with diabetes mellitus. Methods. Experiments were carried out on 100 male white rats that were divided into the following groups: the first group (n=10) - control group consisted of 10 intact animals, the second group (n=40) - rats with simulated traumatic cranial injury, the third group (n=10) - rats with experimental diabetes mellitus, the fourth group (n=40) - rats with simulated traumatic cranial injury and experimental diabetes mellitus. Experimental diabetes mellitus was induced by a single streptozotocin solution intraperitoneal injection. Animals were withdrawn from the experiment at 3, 24 hours, 5 and 14 days after the traumatic cranial injury. The mathematical analysis of the cardiac rhythm variability was performed by ECG intervals data analysis. Corticosterone serum levels were measured using ELISA. Results. Based on a comprehensive ECG intervals data analysis, it was found that after experimental traumatic cranial injury the imbalance of autonomic nervous system with increased sympathetic autonomic nervous system function occurs, manifesting as a deregulation of cholinergic and adrenergic effects on sinoatrial node. In diabetes, an increase of the role of sympathetic effects on the sinus node functioning also occurred. Revealed autonomic nervous system deregulation after experimental traumatic cranial injury associated with experimental diabetes mellitus were associated with sympathetic autonomic nervous system over-function, parasympathetic autonomic nervous system exhausting and decreased recovery speed. There was a relevant increase in corticosterone serum concentration reaching its maximum 24 hours after traumatic cranial injury. Conclusion. A significant autonomic nervous system sympathetic shift and autonomic regulation intensifying characterizes the clinical course in traumatic cranial injury associated with diabetes mellitus, a positive correlation of corticosterone serum concentration and sympathetic autonomic nervous system over-function was revealed in animals with experimental traumatic cranial injury, particularly when associated with experimental diabetes mellitus.

Effect of Curcuma longa and Galega orientalis on renal function in rats with experimental diabetes mellitus and acute renal failure

2019 ◽  
pp. 88-95
Author(s):  
Р.И. Айзман ◽  
А.П. Козлова ◽  
Е.И. Гордеева ◽  
М.С. Головин ◽  
Г.А. Корощенко ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Renal Failure ◽  
Renal Function ◽  
Acute Renal Failure ◽  
Experimental Diabetes ◽  
Curcuma Longa ◽  
Diabetic Rats ◽  
Standard Diet ◽  
Experimental Diabetes Mellitus ◽  
Galega Orientalis

Цель - исследование влияния куркумы длинной и галеги восточной на осмо- и ионорегулирующую функции почек крыс при аллоксан-индуцированном сахарном диабете и острой почечной недостаточности в эксперименте. Методика. Эксперименты выполнены на самцах крыс Wistar (n=70) с моделью сахарного диабета (1-я серия) и острой почечной недостаточности (2-я серия). В обеих сериях животные были поделены на 3 группы: крыс 1-й группы содержали на стандартном корме, крысам остальных групп в корм добавляли куркуму (2-я группа) или галегу (3-я группа) (2% от массы корма). На 7-е сут эксперимента проводили исследование диуретической и ионоуретической функций почек натощак и после 5% водной нагрузки. Концентрацию ионов в моче и плазме определяли методом пламенной фотометрии; осмотическую концентрацию биологических жидкостей - методом криоскопии; биохимические показатели крови - колориметрическим методом. Результаты. У животных с сахарным диабетом фоновый диурез, а также экскреция натрия и калия были статистически значимо выше, чем у контрольных животных. При острой почечной недостаточности наблюдался более низкий уровень диуреза и ионоуреза, особенно после водной нагрузки. Прием куркумы и галеги вызывал улучшение осмо- и ионорегулирующей функции почек у крыс с сахарным диабетом, и практически не влиял на эти функции почек при острой почечной недостаточности. Заключение. При сахарном диабете оба фитопрепарата вызывали понижение концентрации глюкозы, креатинина, мочевины и улучшение ионно-осмотических показателей плазмы крови, при этом эффект куркумы был выражен отчетливее. При острой почечной недостаточности эти фитопрепараты не давали описанного эффекта. Aim. To study effects of the phytomedicines, Curcuma longa and Galega orientalis, on osmosis- and ion-regulating renal functions in rats with experimental diabetes mellitus (DM) and acute renal failure (ARF). Methods. Experiments were performed in two series on Wistar male rats (n=70) with modeled diabetes mellitus (series 1) and acute renal failure (series 2). In each series, the animals were divided into 3 groups, 1) rats of group 1 receiving a standard diet; 2) rats of groups 2 and 3 receiving a standard diet supplemented with turmeric or galega (2% of food weight), respectively. On the 7th day of the experiment, the diuretic and ionuretic renal function was studied in fasting state and after 5% water loading. Concentrations of ions in urine and plasma were determined by flame photometry; osmotic concentrations of biological fluids were measured by cryoscopy; blood biochemical parameters were measured by colorimetry. Results. In diabetic rats, background diuresis and sodium and potassium excretion were significantly higher than in the control animals. In rats with acute renal failure, diuresis and ionuresis were significantly lower, particularly after the water loading. Turmeric and galega supplementation improved the osmotic and ion-regulating renal function in diabetic rats and left practically unchanged these functions in rats with acute renal failure. Conclusion. In rats with diabetes mellitus, both herbal remedies reduced concentrations of glucose, creatinine, and urea and improved ion-osmotic parameters of blood plasma with a more pronounced effect of turmeric. In acute renal failure, these phytomedicines did not produce the described effects.

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