scholarly journals Influence of thePENTAPHYLLOIDES FRUTICOSA l.lInIment on tba-actIve products and antIoxIdant status In wound InjurIes assocIated wIth experImental dIabetes mellItus

2016 ◽  
Vol 1 (5) ◽  
pp. 126-128
Author(s):  
Бальхаев ◽  
Mikhail Balkhaev ◽  
Гармаев ◽  
Bato-Tsyren Garmaev ◽  
Хитрихеев ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Experimental Diabetes ◽  
White Male ◽  
Control Group ◽  
Experimental Diabetes Mellitus ◽  
Male Wistar Rats ◽  
Pentaphylloides Fruticosa ◽  
Experimental Group

The aim of the work was to evaluate the influence of the liniment from Pentaphylloides fruticosa L. on lipid peroxidation in rats with experimental diabetes mellitus accompanied by wound process.The liniment was developed with the use of NanoDeBee30 device. Experiments were carried out on white male Wistar rats. Experimental diabetes mellitus was simulated by a single intraperitoneal introduction of alloxan in a dose of 100mg/kg. The skin injury was simulated by inflicting planar wounds 2.0×2.0cm in size. 5mg of the 5%-liniment from Pentaphylloides fruticosa was put on the wounds of the first experimental group once a day for 14 days. The sec-ond experimental group was treated by the preparation of comparison – balsamic liniment. The wounds of the control group were treated with glycerin-lanolin ointment. On the 7th, 14th and 21st day the concentration of TBA-active products in the blood serum was determined to estimate the intensity of lipid peroxidation processes; the contents of reduced glutathione and catalase activity were determined to evaluate the antioxidant status. The results of the experiments verify the marked curative effect of the liniment from Pentaphylloides fruticosa L. on the healing of the wounds on the background of experimental diabetes mellitus in rats

scholarly journals Comparative characteristics of the manifestations of damage and reparative processes in the mucous membrane of the duodenum of rats under the conditions of skin burns and skin burns associated with diabetes

2020 ◽  
Vol 26 (2) ◽  
pp. 39-44
Author(s):  
I.A. Tymoshenko ◽  
L.M. Sokurenko ◽  
A.Ya. Yanchyshyn ◽  
V.A. Pastukhova
Keyword(s):  
Diabetes Mellitus ◽  
Mucous Membrane ◽  
Structural Changes ◽  
Burn Injury ◽  
Experimental Diabetes ◽  
Male Rats ◽  
Control Group ◽  
Experimental Diabetes Mellitus ◽  
Buffer Solution ◽  
Solution Ph

Currently, severe thermal injury is becoming one of the most important problems of practical medicine. Diabetes is also recognized as another global medical and social challenge of our century. The emergency situation for the treatment and prevention of the consequences of these pathologies is a consequence of the lack of a reliable theoretical basis for solving specific clinical problems regarding the course of burns, diabetes and their complications. The aim of the study is to establish the patterns of structural changes in the mucous membrane of the duodenum after burn injury of the skin of rats under conditions of experimental diabetes mellitus. The study was performed on 63 laboratory white adult male rats weighing 180-210 g, which were divided into 3 groups: intact animals, rats with skin burns and rats with skin burns on the background of diabetes. The model of experimental diabetes mellitus was reproduced by administering Streptozotocin to rats intraperitoneally once at a dose of 50 mg/kg, pre-dissolved in 0.1 M citrate buffer solution (pH=4.5). The control of the development of hyperglycemia in the experimental groups was the level of glucose in the blood 24.24±0.79 mmol/l. In the control group this index was 8.03±0.4 mmol/l. Rats with skin burns revealed destructive manifestations, which are accompanied by an active inflammatory reaction and corresponding necrotic changes, while rats with skin burns on the background of diabetes mellitus pathological processes are not just “summed up”, but in some way adaptively modified with the involvement of stress mechanisms of the endoplasmic reticulum and associated autophagy.

scholarly journals Terminal ileum submucous plexus: Study of the VIP-ergic neurons of diabetic rats treated with ascorbic acid

2002 ◽  
Vol 60 (1) ◽  
pp. 32-37 ◽  
Author(s):  
Jacqueline Nelisis Zanoni ◽  
Luzmarina Hernandes ◽  
Roberto Barbosa Bazotte ◽  
Marcílio Hubner de Miranda Neto
Keyword(s):  
Diabetes Mellitus ◽  
Ascorbic Acid ◽  
Glycated Hemoglobin ◽  
Experimental Diabetes ◽  
Diabetic Rats ◽  
The Other ◽  
Control Group ◽  
Small Reduction ◽  
Experimental Diabetes Mellitus ◽  
Submucous Plexus

The aim of this study was to evaluate the effect of the ascorbic acid (AA) supplementation on the neurons that produce the vasoactive intestinal peptide (VIP) in the submucous plexus of the ileum of rat, four months after the induction of experimental diabetes mellitus with streptozotocin. Three groups of rats were used: C - control, D - diabetic, DA - diabetic receiving AA. We have measured the immunoreactivity and area of 80 cellular bodies of VIP-ergic neurons from each studied group. In the diabetic animals, we have observed hyperphagia, polydipsia, and an increase of glycemia and glycated hemoglobin. The VIP-ergic neurons have presented an increase of their immunoreactivity and the highest profiles when compared to the other groups. In the diabetic animals supplemented with AA it has been observed a small reduction in the glycemia and the water and food intake. We have also noticed smaller immunoreactivity in their VIP-ergic neurons, similar to what we have observed in the control group animals (group C).

scholarly journals Mitochondrial oxidation in the internal organs of rats with cranial injury and diabetes mellitus

2013 ◽  
Vol 94 (5) ◽  
pp. 621-627
Author(s):  
V M Meretskyy
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Succinate Dehydrogenase ◽  
Cytochrome Oxidase ◽  
Experimental Diabetes ◽  
Oxidative Modification ◽  
Experimental Diabetes Mellitus ◽  
Mitochondrial Oxidation ◽  
Potassium Atpase ◽  
Cranial Injury

Aim. To study the relation between the processes of mitochondrial oxidation and the intensity of lipid peroxidation, pulmonary and cardiac proteins oxidative modification at cranial injury, diabetes mellitus and at both. Methods. Experiments were carried out on 100 male white rats distributed to the following groups: the first group (n=10) - control group consisted of 10 intact animals, the second group (n=40) - rats with simulated cranial injury, the third group (n=10) - rats with experimental diabetes mellitus, the fourth group (n=40) - rats with simulated cranial injury and experimental diabetes mellitus. Experimental diabetes was induced by a single intraperitoneal injection of streptozotocin solution. Animals were withdrawn from the experiment at 3, 24 hours, 5 and 14 days after the cranial injury. Cardiac and pulmonary energy supply was estimated by the activity of succinate dehydrogenase, cytochrome oxidase and hydrogen potassium ATPase, as well as by the level of adenine nucleotides. Intensity of free-radical protein and lipid peroxidation was assessed by measuring the levels of neutral and base aldehyde and ketone derivatives and active products reacting with thiobarbituric acid. Results. Decrease of succinate dehydrogenase, cytochrome oxidase activity, a significant increase of hydrogen potassium ATPase activity in heart and lungs mitochondria was observed in rats with cranial injury, diabetes mellitus and especially with both conditions. Adenosine triphosphate tissue reserves have significantly decreased, while adenosine diphosphate and monophosphate levels increased, lipid peroxidation and protein oxidation processes activated. Conclusion. In rats with cranial injury associated with diabetes mellitus, lipid and protein peroxidation intensity parameters, the intensity of oxidative stress and the levels of macroergic substanses were significantly worse compared to the same parameters in the injured animals with normal blood glucose level.

Effect of Sodium Metabisulfite on Oxidative Stress and Lipid Peroxidation Biomarkers

2020 ◽  
Vol 16 (1) ◽  
pp. 114-117
Author(s):  
Shahnaz Shekarforoush ◽  
Parisa Ebrahimi ◽  
Akbar Afkhami Fathabad ◽  
Elaheh Farzanfar
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Treated Group ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Sodium Metabisulfite ◽  
Male Wistar Rats ◽  
Pharmaceutical Agents ◽  
Cellular Components ◽  
Dose Dependent

Background: Sulfites are widely used as preservatives in the foods and pharmaceutical agents. It has been demonstrated that sulfites can react with a variety of cellular components and cause toxicity. Objective: The present study was designed to investigate the effects of ingested sodium metabisulfite (SMB) on serum antioxidant status in rats. Methods: Thirty-two male Wistar rats were randomly divided into control and treated groups. Treated groups received 10, 100, and 260 mg/kg body weight of SMB for 28 days. After 28 days, serum was assayed for measuring superoxide dismtase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) activities, glutathion (GSH) level and lipid peroxidation. Results: The results showed that the activities of GPx, GR, CAT and GSH levels were significantly decreased in 100 and 260 mg/kg SMB treated rats, while malondialdehyde (MDA) level was significantly increased in 260 mg/kg treated group when compared with the control group. Conclusion: It is concluded that SMB administration as dose-dependent is associated with decreased serum antioxidant enzyme activities and increased lipid peroxidation.

scholarly journals The Impact of Type II Diabetes on Tongue Dysplasia and p16-Related Aging Process: An Experimental Study

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Eren Altun ◽  
Hasmet Yazici ◽  
Erhan Arslan ◽  
Kamil Gokce Tulaci ◽  
Haydar Ali Erken
Keyword(s):  
Diabetes Mellitus ◽  
Aging Process ◽  
Experimental Diabetes ◽  
Control Group ◽  
Sprague Dawley ◽  
Experimental Diabetes Mellitus ◽  
Significant Difference ◽  
Filiform Papillae ◽  
The Impact ◽  
P16 Expression

Objective. To evaluate the effect of streptozotocin-induced experimental diabetes mellitus on p16, p53, Ki67, and Bcl2 expressions and histopathological changes in the tongue of the rats. Material and Methods. Twenty-two adult female Sprague-Dawley rats were used. The rats were randomly divided into 2 groups (n=14) as control (C) (n=8) and diabetic (DM) (n=6). The rats in the DM group were given streptozotocin as a single intraperitoneal dose for induction of diabetes. Histopathological and immunohistochemical evaluations of formalin-fixed and paraffin-embedded tissue sections of the tongue were used. Results. Significant differences were observed between the DM group and the control group in terms of epithelial thickness, length of filiform papillae, and width of filiform papillae (p=0.005, p=0.001, and p=0.006, respectively). There was no significant difference between the groups in terms of mononuclear inflammatory cell infiltration, capillary proliferation, and dysplasia (p=0.204, p=0.244, and p=0.204, respectively). As a result of immunohistochemical studies, no significant difference was found between the groups in terms of p53, Ki67, and Bcl-2 expressions (p=0.588, p=0.662, and p=0.686, respectively). A significant difference was found between the groups when p16 expression was evaluated (p=0.006). Conclusions. In our study, streptozotocin-induced experimental diabetes mellitus induced p16 expression but did not show any difference in p53, Bcl-2, and Ki67 levels. It should be considered in the studies that the pathological changes at the early stages of the relationship between DM and oral cancer may be related to p16 expression; however, it may also be linked with p16-related aging process.

scholarly journals Pathomorphological picture of diabetic nephropathy in experimental diabetes mellitus

2019 ◽  
Vol 100 (1) ◽  
pp. 147-152 ◽  
Author(s):  
S O Filinova ◽  
A Yu Zharikov ◽  
I P Bobrov ◽  
O N Mazko ◽  
O G Makarova
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Experimental Diabetes ◽  
Physiological Saline ◽  
Physiological Solution ◽  
Control Group ◽  
Renal Glomerulus ◽  
Experimental Diabetes Mellitus ◽  
Ether Anesthesia ◽  
Renal Glomeruli

Aim. To conduct a morphological study of the renal glomerulus in experimental diabetes mellitus and to study the pathomorphological features of the development of diabetic nephropathy. Methods. The study was carried out on 25 male Wistar rats. Modeling of diabetes mellitus was carried out by intraperitoneal injection of streptozotocin at a dose of 65 mg/kg. For more selective modeling of type 2 diabetes, the rats of the control group were intraperitoneally administered a solution of cytoflavin, calculated by the dosage of nicotinamide 115 mg/kg. In the comparison group 1 ml of physiological solution was administered in a similar manner. On day 28 of the experiment, the animals were euthanized under ether anesthesia, the kidneys were extracted, purified and washed with physiological saline. The material was fixed in a 10% neutral formalin solution. Using the morphometric method, the area of the renal glomeruli and the area of the capillary lumens were measured, and after special computer processing of digital photographs, the total area of the vascular bed in the glomerulus and the area of mesangium in the renal glomerulus were estimated. Results. After four weeks of experiment on rats with diabetes mellitus, some characteristic changes in the morphofunctional state of renal tissues developed: an increase of the area of renal glomeruli, thickening of the basement membrane of glomerular capillaries, an increase in the size of podocytes with a decrease of their number, and foci of nephrosclerosis. Conclusion. Based on the results of the study, characteristic pathomorphological phenomena in the kidneys of rats with experimental diabetes mellitus were recorded, which indicate the development of diabetic nephropathy.

scholarly journals Prevention of NAFLD development in rats with obesity via the improvement of pro/antioxidant state by cerium dioxide nanoparticles

2016 ◽  
Vol 89 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Nazarii Kobyliak ◽  
Ludovico Abenavoli ◽  
Tetyana Falalyeyeva ◽  
Oleksandr Virchenko ◽  
Belemets Natalia ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cerium Dioxide ◽  
Morphological Changes ◽  
Monosodium Glutamate ◽  
White Male ◽  
Antioxidant Properties ◽  
Saline Control ◽  
Control Group ◽  
Nonalcoholic Fatty Liver ◽  
Male Wistar Rats

Background. One of the pathogenic mechanisms of the nonalcoholic fatty liver disease (NAFLD) is the accumulation of reactive oxygen species, which in turn aggravates the disease progress. We have investigated novel cerium dioxide nanoparticles (nCeO2) due to their promising antioxidant auto-regenerative ability and low toxicity.Methods. 30 white male Wistar rats were divided into 3 groups: control, monosodium glutamate (MSG)-induced obesity and MSG treated with nCeO2 (MSG+nCeO2) groups. Newborn rats of control group were injected with saline (control). MSG- and MSG+nCeO2 groups were injected with MSG (4 mg/g concentration, 8 µl/g volume) between the 2nd and the 10th days of life subcutaneously [13]. At the age of 1 month, rats of group II were administered water 2.9 ml/kg orally, MSG+nCeO2 group received 1 mM solution of nCeO2 1 mg/kg orally. 4-months rats were sacrificed and the liver was harvested for histological and biochemical analysis. To assess the morphological changes in the liver we used NAS (NAFLD activity score). The content of lipid peroxidation products and enzymatic activity of superoxide dismutase (SOD) and catalase in the liver were studied by standard biochemical methods [Refs].Results. In 4-month rats we found significantly lower total score (1.3±0.26 vs 3.6±0.34, p<0.001), degree of steatosis (1.1±0.18 vs 2.1±0.18, p<0.001), manifestation of lobular inflammation (0.2±0.13 vs 1.2±0.2, p<0.001) and ballooning degeneration (0.0±0.0 vs 0.3±0.15, p=0.034) due to NAS in the nCeO2 group compared to the MSG-group. nCeO2 significantly decreased lipid peroxidation in the liver tissue, namely it reduced the conjugated dienes content by 27% (p<0.05), TBA-products – by 43% (p<0.05) and Schiff bases – by 21% (p<0.05).Conclusions. Due to its antioxidant properties nCeO2 significantly reduces the incidence of NASH and improves the main NAFLD histological features.

scholarly journals Influence of “Thireomagnile” and “Thyrioton” preparations on the antioxidant status of pregnant cows with symptoms of endotoxicosis

2020 ◽  
Vol 10 (1) ◽  
pp. 122-126 ◽  
Author(s):  
Y. Grymak ◽  
O. Skoromna ◽  
O. Stadnytska ◽  
O. Sobolev ◽  
B. Gutyj ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant System ◽  
Antioxidant Status ◽  
Clinical Signs ◽  
Feed Additives ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
National University ◽  
Pharmacology And Toxicology ◽  
Experimental Group

The purpose of this search was to investigate the influence of “Thireomagnile” and “Thyrioton” on the antioxidant status of cows in the development of endotoxicosis. The experimental part of the work was performed at the Department of Pharmacology and Toxicology of Stepan Gzhytskyj Lviv National University of Veterinary Medicine and Biotechnologies, in the laboratory of Pharmacology and Toxicology of the State Scientific Research Institute of Veterinary Preparation and Feed Additives (Lviv) and in the Ltd. “Barkom”, village Yampil of Pustomyty district, Lviv region. To solve the tasks of the research, three experimental groups of 10 pregnant cows of the Ukrainian Black-Spotted Dairy breed were formed in each: control and two experimental. Cows of the control group (K) had characteristic clinical signs of endotoxicosis. Animals of the first experimental group (E1) were given 10 mL per animal of “Thyroid magnet” at the eighth and ninth months of gestation. The animals of the second experimental group (E2) were given Thyrioton at a dose of 10 mL per animal in the eighth and ninth months of gestation. As a result of the conducted researches it is found out that in pregnant cows with clinical manifestation of endotoxicosis the inhibition of the activity of the antioxidant protection system and intensification of processes of lipid peroxidation occurs. The use of “Thireomagnile” and “Thyrioton” in cows by the development of endotoxicosis contributed to the increased activity of the antioxidant system, which is indicated by an increase in the activity of glutathione peroxidase, glutathione reductase and glucose-6-phosphate dehydrogenase. The use of of “Thireomagnile” and “Thyrioton” to cows in the development of endotoxicosis contributed to the suppression of lipid peroxidation processes, which is indicated by the low level of primary and final products of lipid peroxidation, namely: lipid hydroperoxides and TBA-active products. Better normalizing action on the equilibrium on the activity of the antioxidant system and the intensity of lipid peroxidation was shown by the introduction of “Thyroid magnet” at a dose of 10 cm3 per animal.

scholarly journals Effects of armadin on hepatic transaminases in rats with acute generalized peritonitis on the background of diabetes mellitus

2021 ◽  
Vol 11 (5) ◽  
pp. 333-338
Author(s):  
M. Regeda ◽  
B. Verveha ◽  
N. Melnyk
Keyword(s):  
Diabetes Mellitus ◽  
Abdominal Cavity ◽  
White Male ◽  
The Body ◽  
Laboratory Animals ◽  
Control Group ◽  
Male Adult ◽  
Generalized Peritonitis ◽  
Streptozotocin Induced Diabetes ◽  
Experimental Group

The article discusses the issues about the effect of the Armadin on the hepatic transaminase in rats with acute generalized peritonitis on the background of diabetes mellitus. The experiment was performed on 48 white male adult rats, which were divided into two groups. The experimental group – animals with simulated acute generalized peritonitis against the background of streptozotocin-induced diabetes with the drug “Armadin” (ethylmethylhydroxypyridine succinate) application (n=24). The control group – animals with simulated acute generalized peritonitis against the background of streptozotocin-induced diabetes without pharmacocorrection (n = 24). Diabetes mellitus was simulated by a single intraperitoneal injection of streptozotocin at the dose of 60 mg/kg. Acute generalized peritonitis was induced with 10% faecal suspension in a dose of 0.5 ml per 100 g of animal weight in the abdominal cavity of laboratory animals by puncture method. Every day for 6 days, rats of experimental group received the drug “Armadin” at the doses of 100 mg/kg. We found a probable decrease in aminotransferase activity on day 3 of the study in rats of the experimental group after administration of Armadin. Thus, ALT activity decreased by 26.7%, and AST activity – by 25.9% compared to the control group. On the 7th day of the study, the activity of ALT in the blood of rats of the experimental group decreased to 0.50 ± 0.03 mmol/l, which was 49.5% lower than the control group of rats in this period of the experiment. Similar changes are observed in the study of AST activity, where, accordingly, it decreased by 48 % relative to control. The positive effect of the drug "Armadin" on the body of rats, with AGP on the background of STZ-induced diabetes, is manifested by the restoration of the functional state of the liver.

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