scholarly journals Everyday Uncertainty Work: Making Sense of Biosynthetic Menthol

2018 ◽  
Vol 4 ◽  
pp. 458 ◽  
Author(s):  
Robert Meckin ◽  
Andrew Balmer

Although much public engagement with the field of “synthetic biology” has been conducted, there remains little work that develops an appreciation of how people make sense of this field and its concomitant promised futures from within their everyday lives. Using a case study, based on the compound “menthol” (a terpenoid from plants) which synthetic biologists have developed for production in E. coli, we explore how people make sense of uncertainties in promised or feared futures. Menthol is already an ingredient in many consumer products and the pre-existing use of such products may frame people’s everyday techniques for understanding biosynthetic menthol, with implications for their appreciation of synthetic biology more generally. We adopted a range of sensory methods, including “pop-up” stalls, sketch research, object elicitation interviews and home tours, to explore the everyday situations in which menthol already figures. Participants used a range of strategies, including deferring judgment, invoking other actors as mediators, using their own bodily experiences and using existing moral repertoires, to respond to biosynthetic possibilities. We deploy the concept of “everyday uncertainty work” and show that it is a useful one for understanding how people’s everyday epistemic cultures of uncertainty are routinely adapted to the anticipation of sociotechnical uncertainties, such as those that accompany promissory accounts of science. The implications for these findings for public engagement with technoscience and uncertainty are discussed.

2018 ◽  
Vol 28 (3) ◽  
pp. 290-304 ◽  
Author(s):  
Robert Meckin ◽  
Andrew Balmer

Public involvement in technological anticipation is a common feature of contemporary sociotechnical innovation. However, most engagements abstract sociotechnical futures, rather than situating them in the everyday practices in which people are routinely engaged. Recent developments in synthetic biology have established the potential for ‘drop in’ replacements for ingredients in consumer products, particularly in flavour and fragrance markets. This article explains how a sensory methodology can be used to explore citizens’ everyday experiences and how these can be used to ground anticipation of possible sociotechnical futures. The article uses a socio-historical approach to analyse and compare two practice domains – caring for families and hygiene and personal care – to show how biosynthetic futures can disrupt existing relations between people, objects and ideas. The implications for conceptualising publics in synthetic biology and for approaches to public engagement and participation are discussed more broadly.


Author(s):  
Peter Hopkins

The chapters in this collection explore the everyday lives, experiences, practices and attitudes of Muslims in Scotland. In order to set the context for these chapters, in this introduction I explore the early settlement of Muslims in Scotland and discuss some of the initial research projects that charted the settlement of Asians and Pakistanis in Scotland’s main cities. I then discuss the current situation for Muslims in Scotland through data from the 2011 Scottish Census. Following a short note about the significance of the Scottish context, in the final section, the main themes and issues that have been explored in research about Muslims in Scotland.


2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Simon J. Moore ◽  
Yonek B. Hleba ◽  
Sarah Bischoff ◽  
David Bell ◽  
Karen M. Polizzi ◽  
...  

Abstract Background  A key focus of synthetic biology is to develop microbial or cell-free based biobased routes to value-added chemicals such as fragrances. Originally, we developed the EcoFlex system, a Golden Gate toolkit, to study genes/pathways flexibly using Escherichia coli heterologous expression. In this current work, we sought to use EcoFlex to optimise a synthetic raspberry ketone biosynthetic pathway. Raspberry ketone is a high-value (~ £20,000 kg−1) fine chemical farmed from raspberry (Rubeus rubrum) fruit. Results  By applying a synthetic biology led design-build-test-learn cycle approach, we refactor the raspberry ketone pathway from a low level of productivity (0.2 mg/L), to achieve a 65-fold (12.9 mg/L) improvement in production. We perform this optimisation at the prototype level (using microtiter plate cultures) with E. coli DH10β, as a routine cloning host. The use of E. coli DH10β facilitates the Golden Gate cloning process for the screening of combinatorial libraries. In addition, we also newly establish a novel colour-based phenotypic screen to identify productive clones quickly from solid/liquid culture. Conclusions  Our findings provide a stable raspberry ketone pathway that relies upon a natural feedstock (L-tyrosine) and uses only constitutive promoters to control gene expression. In conclusion we demonstrate the capability of EcoFlex for fine-tuning a model fine chemical pathway and provide a range of newly characterised promoter tools gene expression in E. coli.


2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Stefano Vecchione ◽  
Georg Fritz

Abstract Background Synthetic biology heavily depends on rapid and simple techniques for DNA engineering, such as Ligase Cycling Reaction (LCR), Gibson assembly and Golden Gate assembly, all of which allow for fast, multi-fragment DNA assembly. A major enhancement of Golden Gate assembly is represented by the Modular Cloning (MoClo) system that allows for simple library propagation and combinatorial construction of genetic circuits from reusable parts. Yet, one limitation of the MoClo system is that all circuits are assembled in low- and medium copy plasmids, while a rapid route to chromosomal integration is lacking. To overcome this bottleneck, here we took advantage of the conditional-replication, integration, and modular (CRIM) plasmids, which can be integrated in single copies into the chromosome of Escherichia coli and related bacteria by site-specific recombination at different phage attachment (att) sites. Results By combining the modularity of the MoClo system with the CRIM plasmids features we created a set of 32 novel CRIMoClo plasmids and benchmarked their suitability for synthetic biology applications. Using CRIMoClo plasmids we assembled and integrated a given genetic circuit into four selected phage attachment sites. Analyzing the behavior of these circuits we found essentially identical expression levels, indicating orthogonality of the loci. Using CRIMoClo plasmids and four different reporter systems, we illustrated a framework that allows for a fast and reliable sequential integration at the four selected att sites. Taking advantage of four resistance cassettes the procedure did not require recombination events between each round of integration. Finally, we assembled and genomically integrated synthetic ECF σ factor/anti-σ switches with high efficiency, showing that the growth defects observed for circuits encoded on medium-copy plasmids were alleviated. Conclusions The CRIMoClo system enables the generation of genetic circuits from reusable, MoClo-compatible parts and their integration into 4 orthogonal att sites into the genome of E. coli. Utilizing four different resistance modules the CRIMoClo system allows for easy, fast, and reliable multiple integrations. Moreover, utilizing CRIMoClo plasmids and MoClo reusable parts, we efficiently integrated and alleviated the toxicity of plasmid-borne circuits. Finally, since CRIMoClo framework allows for high flexibility, it is possible to utilize plasmid-borne and chromosomally integrated circuits simultaneously. This increases our ability to permute multiple genetic modules and allows for an easier design of complex synthetic metabolic pathways in E. coli.


NanoEthics ◽  
2020 ◽  
Vol 14 (3) ◽  
pp. 271-283
Author(s):  
Christopher Nathan ◽  
Stuart Coles

AbstractIt has become a standard for researchers carrying out biotechnology projects to do a life cycle assessment (LCA). This is a process for assessing the environmental impact of a technology, product or policy. Doing so is no simple matter, and in the last decades, a rich set of methodologies has developed around LCA. However, the proper methods and meanings of the process remain contested. Preceding the development of the international standard that now governs LCA, there was a lively debate in the academic community about the inclusion of ‘values’ within the process. We revisit this debate and reconsider the way forward for LCA. We set out ways in which those outside of science can provide input into LCAs by informing the value assumptions at stake. At the same time, we will emphasize that the role of those within the scientific community need not (and sometimes, will inevitably not) involve value-free inquiry. We carry out this exploration through a case study of a particular technology project that sought ways to produce industrial and consumer products from algal oils.


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