Sperm motility evaluation following long-term storage (5 years) of cryopreserved sea bream (Sparus aurata L., 1758) semen

2015 ◽  
Vol 31 ◽  
pp. 104-107 ◽  
Author(s):  
A. Fabbrocini ◽  
R. D'Adamo ◽  
S. Pelosi ◽  
L. F. J. Oliveira ◽  
F. Del Prete ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sparus Aurata ◽  
Sea Bream ◽  
Long Term Storage ◽  
Term Storage

scholarly journals ANALYSIS OF CRYOСONSERVED SPERM OF SIRES OF THE LEBEDINIAN BREED AND ORIGINAL BROWN CATTLE OF GERMANY

2019 ◽  
Vol 58 ◽  
pp. 95-101
Author(s):  
V. I. Ladyka ◽  
Y. I. Sklyarenko ◽  
Y. M. Pavlenko ◽  
O. V. Sherbak
Keyword(s):  
Sperm Motility ◽  
Sperm Production ◽  
Long Term Storage ◽  
Brown Cattle ◽  
Cryopreserved Sperm ◽  
Analysis System ◽  
Quantitative Indicators ◽  
Term Storage

In recent years, in Ukraine, as well as around the world, some local breeds have been on the verge of extinction, resulting in irretrievable loss of valuable genes, reduction of genetic diversity. In order to avoid these problems, it is necessary to work on the conservation of animal genetic resources. The creation of banks for long-term storage of biological material is one of the methods of preserving the gene pool of local, small and indigenous breeds of animals. Sperm quality is one of the main factors that determines the success of insemination of heifers and cows. Comprehensive analysis of sperm includes a large number of qualitative and quantitative indicators, the accuracy of which is influenced by a number of objective and subjective factors. Currently, microscopic analysis of sperm motility and survival is used in production conditions. Such approaches are easy to implement, but their results can be subjective. Research using a computer-based sperm fertility analysis system is becoming a priority today. With the help of CASA-Sperm Vision it is possible to carry out a morphological analysis already during the study of sperm motility. The purpose of our research was to analyze the quality of deep-frozen sperm of sires of Lebedinian breed and original brown cattle of Germany in order to attract such genetic material in the conservation of local Lebedinian breed cattle. Materials and methods. In the study used cryopreserved sperm doses of five sires of Lebedinian breed and three sires of the original brown cattle of Germany. Researches of qualitative, quantitative and dynamic characteristics of sperm of bulls were carried out at the laboratory of biotechnology of reproduction of Institute of Animal Breeding and Genetics nd. a. M.V.Zubets of NAAS and production laboratory "Ukrainian genetic company" using computer analysis of sperm motility Sperm Analysis System Version 12 IVOS. It was determined that the average dose of cryopreserved sperm of bulls of the Lebedinian breed was 0.25 ml, and of the sires of the original brown cattle of Germany -0.21 ml. It was found that the sires of the original brown cattle of Germany had the best indicators of total sperm motility by 21.7% compared to the sires of the Lebedinian breed (55.7%). They also noted a greater number of sperm with straight-forward movement of 16.5%. (40.2% of the Lebedinian breed sires). The average concentration of sperm in one milliliter was 10.5 times higher in animals of the Lebedinian breed (compared to the sires of the original brown cattle of Germany (331 million/ml). Comparing the indicators of the average speed of the sperm on the average trajectory (VAP), it should be noted that the minimum value was 85.3 microns/sec, the maximum value for this indicator – 141.7 microns/sec. The results of the VSL study showed that the average value of this indicator of sperm in the studied sperm doses of sires of the Lebedinian breed was 102 microns/sec, of the original brown cattle of Germany – 75. Obtained opposite to the direction coefficients of the correlation depending on the origin, between total sperm motility and average sperm speed for the average trajectory, the average speed of the sperm on the real trajectory, the average deviation of the sperm, the degree of straightness of the directed movement of sperm. Summary. 1. The complex of researches provided an objective analysis of qualitative and quantitative indicators of cryopreserved sperm production of the bulls of original brown cattle of Germany and the Lebedinian breed. It was found that the quality of the studied sperm doses met the requirements of "Instructions for Artificial Insemination of Cows and Heifers" (Order of the Ministry of Agrarian Policy of Ukraine dated August 1, 2001 № 230). 2. The comparative analysis of sperm production of the original brown cattle of Germany and the Lebedinian breed for its long-term storage was carried out. It is established that the sperm of sires of the original brown cattle of Germany exhibit higher dynamic characteristics of movement, while the sperm of sires of the Lebedinian breed were inferior to them in these indicators. 3. The possibility of insemination of females with cryopreserved sperm of the studied bulls whose sperm production has been stored for more than 30 years has been proved.

Inter- and intra-breed comparative study of sperm motility and viability in Iberian and Duroc boar semen during long-term storage in MR-A and XCell extenders

2013 ◽  
Vol 139 (1-4) ◽  
pp. 109-114 ◽  
Author(s):  
D. Martín-Hidalgo ◽  
F.J. Barón ◽  
A. Robina ◽  
M.J. Bragado ◽  
A. Hurtado de Llera ◽  
...  
Keyword(s):  
Comparative Study ◽  
Sperm Motility ◽  
Long Term Storage ◽  
Boar Semen ◽  
Term Storage

Effect of long term storage in LN2 on bacterial load and preservability of semen in Murrah bulls

2015 ◽  
Author(s):  
G. S. Meena ◽  
V. S. Raina ◽  
M. Bhakat ◽  
T. K. Mohanty ◽  
A. K. Gupta ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Incubation Period ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Bacterial Load ◽  
Storage Period ◽  
Least Square ◽  
Long Term Storage ◽  
Term Storage

Successful preservation of semen for longer period can be achieved by maintaining the viability of spermatozoa from its collection till its use for insemination. Therefore, we perceived the idea to determine the effect of long term storage of cryopreserved buffalo semen on sperm quality, bacterial load and fertility. Twenty years semen samples (1981-2000) from twenty bulls and the data on conception rates of these bulls were collected from record room at ABRC, ICAR- NDRI, Karnal. The data was analyzed using least square analysis. The differences in individual motility percent in the semen between estimated initially at the time of freezing and estimates after storage (at the time of evaluation) were 6.49, 14.09, 13.13 and 6.02 percent, respectively. Even after long term storage (up to 20 years) there were little changes in the sperm motility percent. Changes in non-eosinophilic sperm count, sperm abnormalities, HOST and acrosome status, were less up to 20 years storage of semen in liquid nitrogen and the differences were non-significant between different years of storage semen in liquid nitrogen. In similar fashion the microbial load in semen was decreased with the increased storage period of semen in liquid nitrogen, but the differences were non significant. The differences in sperm motility percent between 370C and room temperature were not significant, but the temperature and incubation period significantly (P<0.01) influenced motility percent estimates. Whereas, temperature-incubation period interaction was not found to be significant. The conception rate and sperm motility was highly and positively correlated (r=0.67 and 0.55) with sperm oocyte attachment. It can be concluded that there was little change occurred in semen quality even after twenty years of storage in liquid nitrogen without affecting fertility of semen.

scholarly journals Queen reproductive tract secretions enhance sperm motility in ants

2016 ◽  
Vol 12 (11) ◽  
pp. 20160722 ◽  
Author(s):  
Joanito Liberti ◽  
Boris Baer ◽  
Jacobus J. Boomsma
Keyword(s):  
Sperm Motility ◽  
Reproductive Tract ◽  
Swimming Performance ◽  
Long Term Storage ◽  
Single Mating ◽  
Leaf Cutting ◽  
Acromyrmex Echinatior ◽  
Term Storage

Queens of Acromyrmex leaf-cutting ants store sperm of multiple males after a single mating flight, and never remate even though they may live for decades and lay tens of thousands of eggs. Sperm of different males are initially transferred to the bursa copulatrix and compete for access to the long-term storage organ of queens, but the factors determining storage success or failure have never been studied. We used in vitro experiments to show that reproductive tract secretions of Acromyrmex echinatior queens increase sperm swimming performance by at least 50% without discriminating between sperm of brothers and unrelated males. Indiscriminate female-induced sperm chemokinesis makes the likelihood of storage directly dependent on initial sperm viability and thus provides a simple mechanism to secure maximal possible reproductive success of queens, provided that initial sperm motility is an accurate predictor of viability during later egg fertilization.

Long-Term Storage In Liquid Nitrogen Modifies Boar Sperm Motility But Is Not Detrimental To Fertility

Cryobiology ◽  
2019 ◽  
Vol 91 ◽  
pp. 176
Author(s):  
Phil Purdy ◽  
Kara Stewart ◽  
Scott Spiller ◽  
Carrie S. Wilson ◽  
Drew Lugar ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Long Term Storage ◽  
Boar Sperm ◽  
Term Storage

scholarly journals Вплив тривалого зберігання кріоконсервованої сперми бугаїв у рідкому азоті на показники якості сперматозоїдів

2013 ◽  
pp. 162-164
Author(s):  
А. О. Ляшенко
Keyword(s):  
Survival Rate ◽  
Genetic Resources ◽  
Sperm Motility ◽  
Current Standard ◽  
Long Term Storage ◽  
Term Storage

Проведено дослідження показників прямолінійно-поступального руху та абсолютного показника ви-живаності деконсервованих сперматозоїдів бугаїврізних порід за умов довготривалого зберігання вБанку генетичних ресурсів. Встановлено високийпозитивний кореляційний зв'язок між показникамирухливості та абсолютним показником виживанос-ті сперматозоїдів (r=0,89) (p<0,001). У результатіпроведених досліджень встановлено, що показникирухливості та виживаності сперматозоїдів бугаївбілоголової української, лебединської, симентальськоїта сірої української порід виявилися вищими чинногоДСТУ в середньому на 15 %. The study of performance straight-forward motility and absolute survival rate of thawed sperm bullsof different breeds in long-term storage in the Bank of genetic resources. It is established positive highcorrelation between the indicators of motility and an absolute survival rate of sperm (r=0,89) (p<0,001).The studies found that the rates of sperm motility and survival of white-headed bull’s ukrainian, lebedinsky,simmental and gray ukrainian breeds were higher than the current standard on average by 15 %.

scholarly journals Effect of long-term storage on induced photon emission of boar spermatozoa

2011 ◽  
Vol 51 (No. 2) ◽  
pp. 61-65 ◽  
Author(s):  
P. Gogol ◽  
B. Szczęśniak-Fabiańczyk
Keyword(s):  
Oxidative Damage ◽  
Sperm Motility ◽  
Photon Emission ◽  
Stress Factors ◽  
Simple Method ◽  
Long Term Storage ◽  
Boar Spermatozoa ◽  
Term Storage

Ultraweak photon emission measurements were found to be a useful tool for investigating the influence of stress factors on cells. In the present study, induced photon emission of boar spermatozoa was investigated using a luminometer during a 12-day storage at 15&deg;C in Biosolwens extender. It was shown that the day of storage had a significant effect on some photon emission parameters. During storage, a significant increase in the Integral, Peak max. and Slope max. parameters and a decline in the T.-half (fall) parameter were observed. A significant correlation was observed between photon emission parameters and sperm motility. In conclusion, the results of the present study indicate that the measurement of induced photon emission can be an alternative, sensitive and relatively simple method for assessing the effect of preservation on oxidative damage to boar spermatozoa. &nbsp;

scholarly journals Effect of different extenders on ram sperm traits during storage

2012 ◽  
Vol 60 (6) ◽  
pp. 111-116 ◽  
Author(s):  
Zdeňka Hegedűšová ◽  
Ladislav Štolc ◽  
František Louda ◽  
Libor Čunát ◽  
Jan Vejnar
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Short Term ◽  
Long Term Storage ◽  
Semen Volume ◽  
Reproduction Season ◽  
Ram Sperm ◽  
Sperm Traits ◽  
Term Storage

The aim of the study was to test commercial extenders used for short-term and long-term sperm preservation. Semen was collected in the reproduction season, i.e. from June to December. The ejaculates were obtained from single services and the routine analysis of the semen was performed immediately after the collection. The examination included semen volume, colour and texture, sperm concentration and motility, ejaculate turbulence and percentage of sperm with abnormal morphology. The semen was diluted with an extender in the ratio of 1:4. The processed semen was transported in an insulated container at 16–18 °C to the laboratory and stored in a stationary thermostat under the same temperature. Sperm motility tests were performed 24, 48, 72 and 96 hours after the placement in to thermostat. Ejaculates diluted with Ovipro, Optidyl, Triladyl and Andromed CSS gave very good results of viability (81.23 %–83.41 %) after 24 hours of storage. After 48 hours, Ovipro, Andromed, Optidyl and Triladyl gave values above 75 %. The Triladyl extender proved to be a good stabilizing agent, showing consistent results during a long-term storage. It was chosen as a control one for overall assessment. Other preservation media did not show any improving or worsening effects. The extender Ovipro showed a high motility effect in the first 48 hours only, and hence it appears to be the best solution for the short-term preservation.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Sign in / Sign up

Export Citation Format

Share Document