Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa

Manuel ÁLVAREZ-RODRIGUEZ; Alejandro VICENTE-CARRILLO; Heriberto RODRIGUEZ-MARTINEZ

doi:10.1262/jrd.2017-141

Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa

Journal of Reproduction and Development ◽

10.1262/jrd.2017-141 ◽

2018 ◽

Vol 64 (4) ◽

pp. 351-360 ◽

Cited By ~ 6

Author(s):

Manuel ÁLVAREZ-RODRIGUEZ ◽

Alejandro VICENTE-CARRILLO ◽

Heriberto RODRIGUEZ-MARTINEZ

Keyword(s):

Long Term Storage ◽

Boar Spermatozoa ◽

Term Storage

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Cryopreservation of boar semen

Czech Journal of Animal Science ◽

10.17221/47/2020-cjas ◽

2020 ◽

Vol 65 (No. 4) ◽

pp. 115-123

Author(s):

Marija Jovičić ◽

Eva Chmelíková ◽

Markéta Sedmíková

Keyword(s):

Animal Species ◽

Semen Quality ◽

Egg Yolk ◽

High Rate ◽

Freezing And Thawing ◽

Long Term Storage ◽

Boar Semen ◽

Boar Spermatozoa ◽

Term Storage

Sperm cryopreservation is the best technology for long-term storage of the semen. However, the damage of boar spermatozoa by cryopreservation is more severe than in other animal species and a standardized freezing protocol for efficient cryopreservation has not been established yet. Semen quality and freezability vary greatly between breeds as well as between individual boars and even the season. Boar spermatozoa are sensitive to low temperatures; they sustain damage and a high rate of mortality and freezing/thawing the boar semen may strongly impair the sperm function and decrease the semen quality. The freezability of boar semen can be influenced by a cryopreservation procedure, and also by using various additives to freezing and thawing extenders such as antioxidants. In order to obtain acceptable results after thawing the boar semen, it is necessary to combine an optimal amount of additives (glycerol, egg yolk, sugars, antioxidants), cooling and warming velocities.

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Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

International Journal of Molecular Sciences ◽

10.3390/ijms21186474 ◽

2020 ◽

Vol 21 (18) ◽

pp. 6474

Author(s):

Lisa Höfner ◽

Anne-Marie Luther ◽

Alessandra Palladini ◽

Thomas Fröhlich ◽

Dagmar Waberski

Keyword(s):

Mass Spectrometry ◽

Seminal Plasma ◽

Semen Analysis ◽

Computer Assisted ◽

Protein Markers ◽

Long Term Storage ◽

Lipidome Analysis ◽

Boar Spermatozoa ◽

Term Storage

Long-term exposure of liquid preserved boar spermatozoa to seminal plasma (SP) can cause dramatic sperm injury. This study examined whether boar specificity exists in the sensitivity of spermatozoa to SP and whether correspondent biomarkers can be identified. Consecutive ejaculates (n = 4–5) collected from 19 boars were centrifuged, diluted with a pH-stablising extender with 10% (v/v) autologous SP and evaluated by computer-assisted semen analysis and flow cytometry. Up until 144 h storage, four boars showed consistently high sperm motility, viability and mitochondria activity, and one boar showed consistently low values. Intra-boar variability was high in the other boars. Screening of SP (n = 12 samples) for protein markers using mass spectrometry identified three protein candidates of which the granulin precursor, legumain and AWN were 0.5 to 0.9 log2-fold less abundant (p < 0.05) in SP-resistant compared to SP-sensitive samples. Lipidome analysis by mass spectrometry revealed 568 lipids showing no difference between the SP-groups. The most abundant lipids were cholesterol (42,442 pmol), followed by phosphatidylserine (20,956 pmol) and ether-linked phosphatidylethanolamine (13,039 pmol). In conclusion, three candidate proteins were identified which might be indicative of SP-tolerance of sperm during long-term storage. Noteworthy, a first lipidomic profile of boar SP is presented.

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Effect of long-term storage on induced photon emission of boar spermatozoa

Czech Journal of Animal Science ◽

10.17221/3910-cjas ◽

2011 ◽

Vol 51 (No. 2) ◽

pp. 61-65 ◽

Cited By ~ 3

Author(s):

P. Gogol ◽

B. Szczęśniak-Fabiańczyk

Keyword(s):

Oxidative Damage ◽

Sperm Motility ◽

Photon Emission ◽

Stress Factors ◽

Simple Method ◽

Long Term Storage ◽

Boar Spermatozoa ◽

Term Storage

Ultraweak photon emission measurements were found to be a useful tool for investigating the influence of stress factors on cells. In the present study, induced photon emission of boar spermatozoa was investigated using a luminometer during a 12-day storage at 15°C in Biosolwens extender. It was shown that the day of storage had a significant effect on some photon emission parameters. During storage, a significant increase in the Integral, Peak max. and Slope max. parameters and a decline in the T.-half (fall) parameter were observed. A significant correlation was observed between photon emission parameters and sperm motility. In conclusion, the results of the present study indicate that the measurement of induced photon emission can be an alternative, sensitive and relatively simple method for assessing the effect of preservation on oxidative damage to boar spermatozoa.  

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Modelling of Moisture Movement in Wood during Outdoor Storage

Nonlinear Analysis Modelling and Control ◽

10.15388/na.2001.6.1.15210 ◽

2001 ◽

Vol 6 (2) ◽

pp. 3-14 ◽

Cited By ~ 23

Author(s):

R. Baronas ◽

F. Ivanauskas ◽

I. Juodeikienė ◽

A. Kajalavičius

Keyword(s):

Experimental Data ◽

Finite Difference ◽

Climatic Conditions ◽

Two Dimensional ◽

Moisture Movement ◽

Finite Difference Technique ◽

Long Term Storage ◽

Space Formulation ◽

Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

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