EFFECTS OF HUMAN GROWTH HORMONE ON THE SECRETION OF RAT GROWTH HORMONE

1980 ◽  
Vol 86 (1) ◽  
pp. 165-169 ◽  
Author(s):  
J. O. WILLOUGHBY ◽  
MARGARET MENADUE ◽  
P. ZEEGERS ◽  
P. H. WISE ◽  
J. R. OLIVER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Male Rats ◽  
Mean Frequency ◽  
Rat Growth ◽  
The Mean

Human growth hormone (hGH) was administered to chronically cannulated male rats and its effect upon the physiological secretory patterns of rat growth hormone (rGH) and prolactin were observed. In comparison with injected control animals, a reduction in the size of spontaneous secretory bursts of rGH was apparent when hormone concentrations were compared 3–6 h after administration of hGH (136·27 ± 27·31 (s.e.m.) v. 76·22 ± 20·98 ng/ml respectively). However, the mean frequency of secretory episodes of rGH was unaltered. It is therefore postulated that endogenous rGH may modulate the amplitude but not the rhythmicity of secretory episodes of rGH.

RADIOAUTOGRAPHIC STUDIES ON THE LOCALIZATION OF 125I-LABELLED HUMAN LUTEINIZING AND GROWTH HORMONE IN IMMATURE MALE RATS

1969 ◽  
Vol 43 (1) ◽  
pp. 105-111 ◽  
Author(s):  
D. M. DE KRETSER ◽  
K. J. CATT ◽  
H. G. BURGER ◽  
G. C. SMITH
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Luteinizing Hormone ◽  
Human Growth Hormone ◽  
Light Microscope ◽  
Human Growth ◽  
Proximal Convoluted Tubule ◽  
Male Rats ◽  
Immature Male ◽  
Parenchymal Cells

SUMMARY Twenty-day-old male rats were injected intraperitoneally with either human luteinizing hormone (HLH) or human growth hormone (HGH) labelled with 125I. The localization of these hormones 1–2 hr. after injection was examined under the light microscope after radioautography. Major sites of localization of labelled LH were the interstitial cells of the testis and the proximal convoluted tubule of the kidney. Some hormone was also present in adipose tissue, hepatic parenchymal cells, the mesothelial lining of the peritoneum and underlying macrophages. HGH was localized principally in the proximal convoluted tubule of the kidney with some hormone present in liver, adipose tissue, and the suprarenal cortex.

Human growth hormone blunts Na2EDTA-induced hypocalcaemia in hyposomatotrophic children

1983 ◽  
Vol 102 (1) ◽  
pp. 11-15
Author(s):  
Allen W. Root ◽  
Gregory E. Duckett ◽  
Margaret Sweetland ◽  
Catherine Livingston ◽  
Edward O. Reiter
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Ionized Calcium ◽  
Total Calcium ◽  
The Mean ◽  
Pre Treatment ◽  
Basal Concentration ◽  
Immunoreactive Parathyroid Hormone ◽  
Ethylenediamine Tetraacetate

Abstract. An infusion of disodium ethylenediamine tetraacetate (Na2EDTA) (0.13 mmol/kg for 2 h) was administered to 10 hyposomatotrophic children prior to and after 6 and 12 months of treatment with human growth hormone (hGH). Total and ionized calcium and immunoreactive parathyroid hormone (iPTH) concentrations were determined. Mean basal total and ionized calcium concentrations did not change during the year of treatment with hGH. The nadir concentrations of total and ionized calcium increased progressively during hGH administration and after 12 months were significantly increased over pre-treatment values (total calcium: pre-treatment 1.85 ± 0.32 (sd) mmol/l, + 12 months 2.10 ± 0.15, P < 0.01; ionized calcium: pre-treatment 0.55 ± 0.31 mmol/l, + 12 months 0.78 ± 0.14, P < 0.05). The mean basal concentration of iPTH increased slightly after 12 months of hGH administration (pre-treatment 72 ± 18 pg/ml, + 12 months 106 ± 71, P < 0.05), but Na2EDTA-evoked secretion of iPTH was not significant altered by hGH.

EFFECT OF INTRAVENOUS ADMINISTRATION OF HUMAN GROWTH HORMONE ON SULPHATION FACTOR ACTIVITY IN SERUM OF HYPOPITUITARY SUBJECTS

1971 ◽  
Vol 66 (3) ◽  
pp. 491-497 ◽  
Author(s):  
Kerstin Hall
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Intravenous Administration ◽  
Human Growth ◽  
Significant Rise ◽  
Mean Difference ◽  
Factor Activity ◽  
The Mean

ABSTRACT Human growth hormone (HGH) administered as an iv injection of 2–4 mg to hypopituitary patients induced a rise in the levels of sulphation factor (SF) in serum. The low basal levels of SF were not changed during the first hour after HGH injection. Not until three hours after injection, when HGH values approached basal values, there was a significant rise in SF. The mean difference of SF at one and at three hours after HGH injection was 0.52 ± 0.11.

STIMULATION OF IMMUNOREACTIVE INSULIN AND HUMAN GROWTH HORMONE RELEASE BY ADMINISTRATION OF ARGININE IN PATIENTS WITH DIABETIC RETINOPATHY

1972 ◽  
Vol 70 (4) ◽  
pp. 719-730 ◽  
Author(s):  
W. Waldhäusl
Keyword(s):  
Growth Hormone ◽  
Diabetic Retinopathy ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Immunoreactive Insulin ◽  
Insulinogenic Index ◽  
Hormone Release ◽  
Arginine Hydrochloride ◽  
The Mean ◽  
Secretory Capacity

ABSTRACT The effect of arginine hydrochloride (30 g), given twice at 90 min intervals, on the release of immunoreactive insulin and of human growth hormone was studied in healthy subjects (HS), in diabetics without and with various degrees of retinopathy and in acromegalics. The insulinogenic index estimated by the ratio of μU IRI per ml/mg per 100 ml of blood glucose was maximal in HS after the first administration of arginine. It was highest in acromegalics and diminished in all the diabetic subjects. The release of human growth hormone as estimated by the mean sum of increments above the basal levels (x̄ + sem) during period I and II was 80.2±15 ng/ml and 59.0±18 ng/ml in HS (n = 7), 38±8.6 ng/ml and 36±16.3 ng/ml in diabetics without retinopathy (n = 9), 4.2±0.4 ng/ml and 19±7 ng/ml in insulin treated diabetics with retinopathy (n = 9), and 22.7±10.7 ng/ml and 46±10.5 ng/ml in orally treated diabetics with retinopathy (n = 7). Patients suffering from proliferative retinopathy (n = 9) exhibited values of 32.8±10.6 ng/ml during period I and 43.5±10.6 ng/ml during period II. The secretory response of HGH to arginine in acromegalics (n = 6) was not significant. The data reported suggest an impaired secretory capacity for the release of human growth hormone to the administration of arginine in patients with diabetic retinopathy. The observations do not support the hypothesis that an exaggerated release of HGH plays a role in the development of diabetic retinopathy.

Effects of oestrogen and human growth hormone on pregnancy-associated plasma proteins in the rat

1987 ◽  
Vol 116 (2) ◽  
pp. 299-304 ◽  
Author(s):  
L. Carlsson-Bostedt ◽  
N. Frölander ◽  
S. Edén ◽  
T. Stigbrand ◽  
B. von Schoultz
Keyword(s):  
Growth Hormone ◽  
Acute Phase ◽  
Human Growth Hormone ◽  
Plasma Proteins ◽  
Human Growth ◽  
Male Rats ◽  
Complement Factor ◽  
Pronounced Increase ◽  
Additive Influence ◽  
Protein Response

Abstract. The serum concentrations of pregnancy-associated murine protein-1 (PAMP-1), acute phase α2macroglobulin, albumin, transferrin, and complement factor 3(C3) were followed in male rats during continuous infusions of oestradiol-17β and human growth hormone. Three different patterns of protein response could be distinguished. A distinct acute phase response without any additive influence of the given hormones was recorded for α2-macroglobulin, whereas the levels of albumin, transferrin and C3 were virtually unaffected throughout the experiment. Growth hormone gave a rapid and pronounced increase of PAMP-1 levels, whereas the response to oestradiol of this 'steroid-sensitive' protein was significantly weaker and delayed. It is suggested that the apparent oestrogenic influence on certain pregnancy-associated plasma proteins is mediated via growth hormone.

OBSERVATIONS ON A HAEMAGGLUTINATION—INHIBITION TECHNIQUE FOR ESTIMATING HUMAN GROWTH HORMONE

1963 ◽  
Vol 26 (2) ◽  
pp. 219-231 ◽  
Author(s):  
P. J. O'CONNOR ◽  
L. G. SKINNER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Haemagglutination Inhibition ◽  
Human Growth ◽  
Mean Value ◽  
Normal Adult ◽  
Adult Human ◽  
Human Sera ◽  
The Mean ◽  
Inhibition Technique

SUMMARY The haemagglutination—inhibition technique has been examined as a method of estimating human growth hormone (HGH) and the need for rigid standardization of the procedures involved is stressed. Examination of antisera to a Raben type preparation by immunodiffusion and haemagglutination—inhibition procedures showed the presence of antibodies to albumin and γ-globulin as well as to HGH. The presence of these contaminating antibodies did not appear to interfere with the endpoints obtained in the haemagglutination—inhibition reactions. Within its limitations the technique has been found suitable for the assay of solutions of purified HGH. The mean level of HGH in six normal adult human sera was estimated as 261 ± 23·6 μg./l. (± s.e.) which is similar to the values obtained by other workers, but the validity of this mean value is questioned.

EFFECT OF GROWTH HORMONE AND NUTRITION ON THE LEVEL OF SOMATOMEDIN A IN THE RAT

1978 ◽  
Vol 87 (3) ◽  
pp. 485-494 ◽  
Author(s):  
Kazue Takano ◽  
Naomi Hizuka ◽  
Koichi Kawai ◽  
Kazuo Shizume
Keyword(s):  
Growth Hormone ◽  
Human Growth ◽  
Mean Value ◽  
Male Rats ◽  
Low Protein ◽  
Adequate Food ◽  
Hypophysectomized Rats ◽  
The Mean ◽  
Fat Diets ◽  
Dose Dependent Increase

ABSTRACT Somatomedin A was determined by radioreceptor assay of serum from rats with various conditions. The mean value of somatomedin A in 28 day old male rats was 6.2 ± 0.44 U/ml. Following hypophysectomy, serum somatomedin A decreased with a half-life of approximately 6 h. After a single ip injection of human growth hormone into hypophysectomized rats, the levels of somatomedin A increased within 4 h and reached the maximal level between 8 and 24 h. There was a dose-dependent increase of somatomedin A at 24 h after injection of hGH when the injected dose was between 3.2 and 80 μg. Serum somatomedin A was significantly decreased after 24 h of fasting to the same levels as after hypophysectomy. However the changes in serum rGH between fasting rats and fed rats did not show any significant differences. After different intakes of diets with different composition for 32 days, the mean levels of serum somatomedin A were 14.5 ± 2.2, 6.22 ± 0.4, 2.5 ± 0.3 and 13.0 ± 1.1 U/ml when control-, high protein-, high carbohydrate-low protein- and high fat diets were given respectively. There was a positive correlation between the per cent increase in weight and the serum level of somatomedin A. These results indicate that not only growth hormone but also adequate food intake is required for the generation of somatomedin.

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