EFFECT OF GROWTH HORMONE AND NUTRITION ON THE LEVEL OF SOMATOMEDIN A IN THE RAT

1978 ◽  
Vol 87 (3) ◽  
pp. 485-494 ◽  
Author(s):  
Kazue Takano ◽  
Naomi Hizuka ◽  
Koichi Kawai ◽  
Kazuo Shizume
Keyword(s):  
Growth Hormone ◽  
Human Growth ◽  
Mean Value ◽  
Male Rats ◽  
Low Protein ◽  
Adequate Food ◽  
Hypophysectomized Rats ◽  
The Mean ◽  
Fat Diets ◽  
Dose Dependent Increase

ABSTRACT Somatomedin A was determined by radioreceptor assay of serum from rats with various conditions. The mean value of somatomedin A in 28 day old male rats was 6.2 ± 0.44 U/ml. Following hypophysectomy, serum somatomedin A decreased with a half-life of approximately 6 h. After a single ip injection of human growth hormone into hypophysectomized rats, the levels of somatomedin A increased within 4 h and reached the maximal level between 8 and 24 h. There was a dose-dependent increase of somatomedin A at 24 h after injection of hGH when the injected dose was between 3.2 and 80 μg. Serum somatomedin A was significantly decreased after 24 h of fasting to the same levels as after hypophysectomy. However the changes in serum rGH between fasting rats and fed rats did not show any significant differences. After different intakes of diets with different composition for 32 days, the mean levels of serum somatomedin A were 14.5 ± 2.2, 6.22 ± 0.4, 2.5 ± 0.3 and 13.0 ± 1.1 U/ml when control-, high protein-, high carbohydrate-low protein- and high fat diets were given respectively. There was a positive correlation between the per cent increase in weight and the serum level of somatomedin A. These results indicate that not only growth hormone but also adequate food intake is required for the generation of somatomedin.

Effect of nutrition on growth and somatomedin A levels in the rat

1980 ◽  
Vol 94 (3) ◽  
pp. 321-326 ◽  
Author(s):  
Kazue Takano ◽  
Naomi Hizuka ◽  
Kazuo Shizume ◽  
Yoko Hasumi ◽  
Toshio Tsushima
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Normal Growth ◽  
Protein Diet ◽  
Low Protein Diet ◽  
High Fat ◽  
Low Protein ◽  
High Fat Diets ◽  
Hypophysectomized Rats ◽  
Fat Diets

Abstract. Serum somatomedin A was significantly reduced after 3 days of fasting in rats with a mean decrease of 23.6 ± 2.4% (N = 18) of initial values. Re-feeding for one day produced a definite increase in somatomedin A, with a rise in body weight. When re-fed isocalorically for 21 days with diets of different quality, a low protein diet led to smaller increases in both seum somatomedin A and body weight in comparison to those of control-, high-protein- and high fat-diets (P < 0.001). There is a positive correlation between the increase in body weight and serum somatomedin A levels (N = 70, r = 0.71, P< 0.001). The effect of growth hormone on somatomedin generation was abolished in hypophysectomized rats fed with low-protein diet. Our study suggests that protein in the diet is important for the generation of somatomedin A, which is necessary for normal growth.

EFFECTS OF HUMAN GROWTH HORMONE ON THE SECRETION OF RAT GROWTH HORMONE

1980 ◽  
Vol 86 (1) ◽  
pp. 165-169 ◽  
Author(s):  
J. O. WILLOUGHBY ◽  
MARGARET MENADUE ◽  
P. ZEEGERS ◽  
P. H. WISE ◽  
J. R. OLIVER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Male Rats ◽  
Mean Frequency ◽  
Rat Growth ◽  
The Mean

Human growth hormone (hGH) was administered to chronically cannulated male rats and its effect upon the physiological secretory patterns of rat growth hormone (rGH) and prolactin were observed. In comparison with injected control animals, a reduction in the size of spontaneous secretory bursts of rGH was apparent when hormone concentrations were compared 3–6 h after administration of hGH (136·27 ± 27·31 (s.e.m.) v. 76·22 ± 20·98 ng/ml respectively). However, the mean frequency of secretory episodes of rGH was unaltered. It is therefore postulated that endogenous rGH may modulate the amplitude but not the rhythmicity of secretory episodes of rGH.

OBSERVATIONS ON A HAEMAGGLUTINATION—INHIBITION TECHNIQUE FOR ESTIMATING HUMAN GROWTH HORMONE

1963 ◽  
Vol 26 (2) ◽  
pp. 219-231 ◽  
Author(s):  
P. J. O'CONNOR ◽  
L. G. SKINNER
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Haemagglutination Inhibition ◽  
Human Growth ◽  
Mean Value ◽  
Normal Adult ◽  
Adult Human ◽  
Human Sera ◽  
The Mean ◽  
Inhibition Technique

SUMMARY The haemagglutination—inhibition technique has been examined as a method of estimating human growth hormone (HGH) and the need for rigid standardization of the procedures involved is stressed. Examination of antisera to a Raben type preparation by immunodiffusion and haemagglutination—inhibition procedures showed the presence of antibodies to albumin and γ-globulin as well as to HGH. The presence of these contaminating antibodies did not appear to interfere with the endpoints obtained in the haemagglutination—inhibition reactions. Within its limitations the technique has been found suitable for the assay of solutions of purified HGH. The mean level of HGH in six normal adult human sera was estimated as 261 ± 23·6 μg./l. (± s.e.) which is similar to the values obtained by other workers, but the validity of this mean value is questioned.

GROWTH RETARDATION: USE OF SULFATION FACTOR AS A BIOASSAY FOR GROWTH HORMONE

PEDIATRICS ◽  
1963 ◽  
Vol 31 (4) ◽  
pp. 538-551
Author(s):  
Maurice D. Kogut ◽  
S. A. Kaplan ◽  
C. S. N. Shimizu
Keyword(s):  
Growth Hormone ◽  
Human Growth ◽  
Hormone Activity ◽  
Normal Children ◽  
Immature Rat ◽  
Pituitary Diseases ◽  
Hypophysectomized Rats ◽  
Normal Human ◽  
The Mean

A bioassay for measurement of human growth hormone based upon the observation that normal human serum, in vitro, will favor the incorporation of S-35 into immature rat cartilage is described. The factor in serum is called "sulfation factor." Sulfation factor determinations were performed on 27 normal children, 15 with hypopituitarism, 26 dwarfs without demonstrable endocrine or other diseases, 11 children with a variety of non-pituitary diseases causing dwarfism, and 1 adult with acromegaly. The mean activity (± S. E.) of sulfation factor was 0.84 ± 0.057 S. F. units in the normal children, 0.45 ± 0.024 S. F. units in idiopathic hypopituitary patients (p &lt; 0.01), 0.95 ± 0.054 S. F. units in dwarfs without evidence of endocrine or other recognizable diseases, 0.82 ± 0.057 S. F. units in dwarfs with well recognized nonpituitary disease, and 2.2 S. F. units in one adult with acromegaly. There was an excellent correlation between sulfation factor and other laboratory determinations of pituitary function. Hypophysectomized rats receiving bovine growth hormone showed substantial increase of sulfation factor as compared to control hypophysectomized rats. Sulfation factor activity appears to be a valid and useful indicator of growth hormone activity in serum.

Reduced and S-carboxymethylated human growth hormone: a probe for diabetogenic action

1984 ◽  
Vol 247 (5) ◽  
pp. E639-E644
Author(s):  
C. M. Cameron ◽  
J. L. Kostyo ◽  
J. A. Rillema ◽  
S. E. Gennick
Keyword(s):  
Growth Hormone ◽  
Amino Acid ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Mouse Mammary Gland ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Hypophysectomized Rats

The biological activity profile of reduced and S-carboxymethylated human growth hormone (RCM-hGH) was determined to establish its suitability for study of the diabetogenic property of hGH. RCM-hGH was found to have greatly attenuated in vivo growth-promoting activity in the 9-day weight-gain test in hypophysectomized rats (approximately 1%) and to have a similar low order of in vitro activity in stimulating amino acid incorporation into the protein of the isolated rat diaphragm. RCM-hGH also only had approximately 1% of the in vitro insulin-like activity of the native hormone on isolated adipose tissue from hypophysectomized rats. In contrast, RCM-hGH retained substantial in vivo diabetogenic activity in the ob/ob mouse, appearing to have approximately 50% of the activity of the native hormone. RCM-hGH was also found to retain significant, although attenuated (25%), in vitro lactogenic activity when tested for the ability to stimulate amino acid incorporation into a casein-rich protein fraction in mouse mammary gland explants. Because RCM-hGH exhibits a high degree of diabetogenic activity, although lacking significant anabolic or insulin-like activities, it will be useful as a "monovalent" probe for the study of the molecular mechanism of the diabetogenic action of GH.

RADIOAUTOGRAPHIC STUDIES ON THE LOCALIZATION OF 125I-LABELLED HUMAN LUTEINIZING AND GROWTH HORMONE IN IMMATURE MALE RATS

1969 ◽  
Vol 43 (1) ◽  
pp. 105-111 ◽  
Author(s):  
D. M. DE KRETSER ◽  
K. J. CATT ◽  
H. G. BURGER ◽  
G. C. SMITH
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Luteinizing Hormone ◽  
Human Growth Hormone ◽  
Light Microscope ◽  
Human Growth ◽  
Proximal Convoluted Tubule ◽  
Male Rats ◽  
Immature Male ◽  
Parenchymal Cells

SUMMARY Twenty-day-old male rats were injected intraperitoneally with either human luteinizing hormone (HLH) or human growth hormone (HGH) labelled with 125I. The localization of these hormones 1–2 hr. after injection was examined under the light microscope after radioautography. Major sites of localization of labelled LH were the interstitial cells of the testis and the proximal convoluted tubule of the kidney. Some hormone was also present in adipose tissue, hepatic parenchymal cells, the mesothelial lining of the peritoneum and underlying macrophages. HGH was localized principally in the proximal convoluted tubule of the kidney with some hormone present in liver, adipose tissue, and the suprarenal cortex.

scholarly journals Human Growth Hormone Induces SOCS3 and CIS mRNA Increase in the Hypothalamic Neurons of Hypophysectomized Rats

2004 ◽  
Vol 51 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Yoko KASAGI ◽  
Reiko TOKITA ◽  
Tomoko NAKATA ◽  
Toshihiro IMAKI ◽  
Shiro MINAMI
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Hypothalamic Neurons ◽  
Hypophysectomized Rats

PREPARATION OF HUMAN GROWTH HORMONE

1961 ◽  
Vol 23 (3) ◽  
pp. 285-NP ◽  
Author(s):  
A. L. C. WALLACE ◽  
K. A. FERGUSON
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
High Yield ◽  
Pituitary Hormone ◽  
Simple Method ◽  
Anterior Pituitary Hormone ◽  
Epiphysial Cartilage ◽  
Diethylaminoethyl Cellulose ◽  
Hypophysectomized Rats

SUMMARY A simple method for the preparation of human growth hormone using chromatography on diethylaminoethyl-cellulose is described. Material prepared in this way, when assayed by growth of the tibial epiphysial cartilage in hypophysectomized rats, is at least as active as material prepared by published methods and is obtained in high yield. The only other anterior pituitary hormone activity present in any concentration is prolactin.

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