Estimation of total phenolic content and Studies on antioxidant activity of different extracts of Cadaba farinose Forsk by hydroxy radical and iron chelating techniques

Cadaba farinosa (family Capparidaceae) is generally known as “Indian cadaba” in the traditional ayurvedic system. The current study, aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Cadaba farinose was evaluated for its in-vitro antioxidant potential by hydroxy radical taking ascorbate as a standard. The iron-chelating activity is taking Ethylenediamine tetraacetate as standard and estimation of total phenol content as equivalent to mg/g of Gallic acid. The methanolic concentrates of Cadaba farinose & ascorbic acid exhibited antioxidant potential possessing IC50 205µg/ml & 65µg/ml (Hydroxy radical) , methanolic concentrates of Cadaba farinose & Ethylenediamine tetraacetate exhibited antioxidant potential possessing IC50 240µg/ml & 70µg/ml (iron-chelating activity). The methanolic and EA concentrates of Cadaba farinose contain total phenolic content 9.86 ± 0.62 and 3.98 ± 0.54, respectively. The IC50 value was originated that methanolic concentrates of Cadaba farinose more efficient in hydroxy radical, iron chelating activity compared EA & PE concentrates. The methanolic extract of Cadaba farinose having more free radical activity due to the presence of phenolic content as a bioactive compound. This result indicates that aerial parts of methanolic concentrate Cadaba farinose could serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

Study of in-vitro antioxidant activity of various extracts of aerial parts of Olax scandens

Olax scandensRoxb. (family Olacaceae) Available in throughout tropical India. The current study, aerial parts of different concentrates of Olax scandenswas evaluated for its in-vitro antioxidant potential by Diphenylpicrylhydrazyl activity, superoxide scavenging activity, iron chelating activity taking ascorbate, quercetin & Ethylenediamine tetraacetate as the standard correspondingly. An IC50 value was originated that methanolic concentrates of Olax scandensis more efficient in Diphenylpicrylhydrazyl radical, superoxide radical activity, Iron chelating capacity compared EA & PE concentrates. The methanolic concentrates of Olax scandens & ascorbate exhibited antioxidant potential possessing IC50 226µg/ml & 66µg/ml (DPPH). 185µg/ml & 60µg/ml (Superoxide) , 287µg/ml & 65µg/ml (iron-chelating Activity) respectively. Invitro antioxidant studies obviously show methanolic concentrates of Olax scandens have better antioxidant activity. These results indicate that aerial parts of methanolic concentrates Olax scandenscould serve as a natural antioxidant, which may be useful in preventing free radical-induced diseases.

Synthesis of Zeolite from Blast Furnace Slag Using Alkali Fusion with Addition of EDTA

A large amount of blast furnace slag, a by-product of the iron-making process, is generated annually. We attempted to convert blast furnace slag (BFS) into zeolitic materials using alkali fusion with ethylenediamine tetraacetate dihydrate (EDTA) chelation. Without addition of EDTA, a mixture of hydroxysodalite and calcite was synthesized. With EDTA addition, Ca2+ ions were trapped by chelation in solution during agitation, then the chelation was promoted by heating at synthesis, and zeolite-A, zeolite-X and hydroxysodalite were synthesized.

DFT studies of complexes between ethylenediamine tetraacetate and alkali and alkaline earth cations

Density functional theory (DFT) calculations for M–(C10H12N2O84–) and M–(H2O)6, where M = Na+, K+, Rb+, Mg2+, Ca2+, and Sr2+, and (C10H12N2O84–) = EDTA4–, were carried out at both all-electron and pseudopotential levels. Results from these four methods were compared, and it was found that the ions bind to EDTA4– with selectivity of Mg2+ > Ca2+ > Sr2+ > Na+ > K+ > Rb+.