scholarly journals ВПЛИВ ДЖЕРЕЛ ВУГЛЕЦЕВОГО ТА АЗОТНОГО ЖИВЛЕННЯ НА БІОДЕГРАДАЦІЮ КСЕНОБІОТИКІВ КУЛЬТУРАМИ КСИЛОТРОФІВ

2013 ◽  
Vol 3 (03) ◽  
pp. 109
Author(s):  
A. V. Chaika ◽  
O. V. Fedotov
Keyword(s):  
Methyl Orange ◽  
Model Compound ◽  
Nitrogen Nutrition ◽  
Submerged Cultures ◽  
Weighting Method ◽  
Trametes Hirsuta ◽  
Carbon And Nitrogen ◽  
Xylotrophic Basidiomycetes ◽  
Peptone Medium ◽  
Biodegradation Efficiency

<p>The effect of different carbon and nitrogen nutrition sources on the efficiency of xenobiotic biodegradation was investigated by means of submerged xylotrophic basidiomycetes cultures. We cultivated the prospective strains of xylotrophic basidiomycetes like <em>Flammulina velutipes</em> F-1105, <em>Pleurotus eryngii</em> P-er, <em>Trametes hirsuta</em> Th-11, and <em>Daedalea quercina</em> Dq-08 on the lab shaker on modified glucose-peptone medium. Some 24 of the carbon and 15 of the nitrogen nutrition sources were investigated. Absolutely dry biomass of the mycelium was determined by weighting method. The pollutants biodegradation efficiency of submerged cultures was determined by a modified method on a model compound – <em>Methyl Orange</em>. The individual carbon- and nitrogen-containing components of the modified glucose-peptone medium, the cultivation on which provides induction of model compound <em>Methyl Orange</em> biodegradation efficiency of the strains was also determined. They mostly were the carbon sources like glucose, fructose, xylose, starch, glycerol, PEG -1500, and the nitrogen sources like urea and peptone. It is suggested that the biodegradation efficiency of induction could caused either by inductive action of the nutrient component on metabolism of culture or by the manifestation of an adaptive response to adverse conditions.</p> <p><em>Keywords: xylotrophic basidiomycetes, biodegradation, Methyl Orange.</em></p>

scholarly journals ДЕСТРУКЦІЯ КСЕНОБІОТИКІВ З ВИКОРИСТАННЯМ КУЛЬТУРАЛЬНОГО ФІЛЬТРАТУ КСИЛОТРОФІВ

2015 ◽  
Vol 5 (03) ◽  
pp. 55-72
Author(s):  
O. V. Fedotov
Keyword(s):  
Methyl Orange ◽  
Sodium Acetate ◽  
Model Compound ◽  
Oxidative Degradation ◽  
Tween 80 ◽  
Degradation Efficiency ◽  
Submerged Cultivation ◽  
Sodium Acetate Buffer ◽  
Xylotrophic Basidiomycetes ◽  
Peptone Medium

<p>The article deals with the efficiency of pollutants biodegradation by xylotrophic basidiomycetes submerged cultures grown on standard glucose-peptone medium (GPM). The efficiency of pollutants biodegradation was determined by the model compound – dye <em>Methyl Orange</em>. The purpose of the work is screening of 19 species 81 strains xylotrophic basidiomycetes cultures on the indicator of the dye oxidative degradation efficiency and exploring the possibility of induction of this indicator by modifying the culture medium. The biodegradation efficiency was determined by following method. Assigned amount of culture filtrate (experiment) or medium (control) was added to the 0.001% solution of <em>Methyl Orange</em> in sodium acetate buffer. pH of the reaction mixture was 4.4 units. Samples were incubated at +40°C for 48 hours. Then pH of the reaction mixture was set up at 3.1 units using sodium acetate buffer and the optical density of solutions at a wavelength of 506 nm was measured. The efficiency of biodegradation was calculated by the difference of the optical density of control and experiment as a percentage. The most promising strains – <em>F. velutipes</em> F-1105, <em>P. eryngii</em> P-er, <em>T. hirsuta</em> Th-11 and <em>D. quercina</em> Dq-08 were selected. The composition of the glucose-peptone medium was modified for these strains by the introduction in the medium lignosulfonate, Tween 80, Kirk’s minerals solution and selecting the concentration of these components. According to the study for the purpose of pollutants degradation it is advisable to cultivate  <em>F. velutipes</em> F-1105 strain on modified GPM, which further comprises at 1 l: lignosulfonate – 3.5 g; Tween 80 – 1.0 g, Kirk’s minerals solution – 70 ml; <em>P. eryngii</em> P-er strain – 5.0 g, 1.0 g, 70 ml; <em>T. hirsuta</em> Th-11 strain – 5.0 g, 1.0 g, 105 ml; and <em>D. quercina</em> Dq-08 strain – 6.5 g, 1.0 g, 105 ml, respectively. This allowed to increase the model compound degradation efficiency by the culture filtrate of strain <em>F. velutipes</em> F-1105 in 9,3; <em>D. quercina</em> Dq-08 – in 9,6; <em>P. eryngii </em>P-er – in 13,3 and T. hirsuta Th-11 – 19,2 times. Thus, GPS modifications were designed that enhance the model compound oxidative degradation efficiency and are the basis for further optimization of the selected xylotrophic basidiomycetes strains submerged cultivation conditions to increase<strong> </strong>biodegradation of xenobiotics.</p> <p><em>Keywords:</em> xylotrophic basidiomycetes, submerged cultivation, biodegradation, <em>Methyl Orange</em>.</p>

scholarly journals КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНІ ХАРАКТЕРИСТИКИ КСИЛОТРОФНИХ БАЗИДІОМІЦЕТІВ У ГЛИБИННІЙ КУЛЬТУРІ

2013 ◽  
Vol 3 (01) ◽  
pp. 128
Author(s):  
A. V. Chaika ◽  
O. V. Fedotov
Keyword(s):  
Submerged Fermentation ◽  
Morphological Characteristics ◽  
Its Region ◽  
Submerged Cultivation ◽  
Submerged Cultures ◽  
Biosynthetic Activity ◽  
Xylotrophic Basidiomycetes ◽  
Weighing Method ◽  
Peptone Medium ◽  
Biomass Increase

<p>The aim of the present study was to evaluate the growth specificities of 79 strains of 18 species xylotrophic basidiomycetes under submerged fermentation on glucose-peptone medium. Most of the strains (85%) were isolated from the fruiting bodies collected in different localities of Donetsk and its region. The macro- and microscopic structure of pellets was studied, pH of the culture filtrate was determined by potentiometric method, ovendry biomass was determined by weighing method and biomass increase and specific growth rate were calculated. The results obtained allowed to ascertain the strain and species character of culture-morphological characteristics of the studied basidiomycetes. On the basis of the research the higher basidiomycetes submerged cultivation method was optimized making the period of fermentation significantly reduced. The growth peculiarities revealed can be used for further studies of biosynthetic activity of the xylotrophic basidiomycetes submerged cultures.</p> <p><em>Key words: xylotrophic basidiomycetes, submerged fermentation, growth and morphological characteristics</em></p>

The Influence of Carbon and Nitrogen Nutrition on Growth and Sterolinduced Sexuality of Pythium sp. PRL 2142

Mycologia ◽  
1969 ◽  
Vol 61 (6) ◽  
pp. 1096 ◽  
Author(s):  
J. J. Child ◽  
G. Defago ◽  
R. H. Haskins
Keyword(s):  
Nitrogen Nutrition ◽  
Carbon And Nitrogen

STUDIES ON THE PARASITISM OF CLADOSPORIUM FULVUM

1967 ◽  
Vol 45 (11) ◽  
pp. 2125-2135
Author(s):  
Thomas Curren
Keyword(s):  
Nitrogen Nutrition ◽  
Chromatographic Study ◽  
Cladosporium Fulvum ◽  
Tomato Plants ◽  
Carbon And Nitrogen ◽  
Amino Compounds ◽  
Race 1 ◽  
Partial Deficiency ◽  
Susceptible Tomato ◽  
Reduced Light

A study was made of the carbon and nitrogen nutrition of races 7, 8, and 10 of Cladosporium fulvum, and an attempt was made to relate the findings to the differential pathogenicity of the races.Cysteine was found to exert a differential effect on the growth of two races of the pathogen. Race 1 was stimulated by concentrations inhibitory to race 6. C. fulvum was found to have a partial deficiency for thiamine. The sporulation of race 10 on susceptible tomato plants was heavier with alanine-grown cultures than with any other nutritional treatment. Altering the nitrogen nutrition of varieties Potentate (S) and V-121 (R) did not affect their differential reactions to race 1. A reduction in nitrogen feeding to Stirling Castle (R) increased its resistance to race 1.A chromatographic study was made of the changes in amino compounds and sugars in Potentate and V-121 after infection by races 1 and 6. The relation of the findings to the resistance of V-121 to race 1 is discussed.The resistance of Stirling Castle was investigated by comparing the amino compounds and sugars in leaves of healthy plants and plants infected by race 1 under optimal conditions and under reduced light, where the resistance breaks down. Chromatographic analyses showed a noticeable depletion of certain metabolites in plants infected under reduced light as compared with the amount of depletion, if any, in the controls.

scholarly journals Conversion of Shrimp Head Waste for Production of a Thermotolerant, Detergent-Stable, Alkaline Protease by Paenibacillus sp.

Catalysts ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 798 ◽  
Author(s):  
Chien Thang Doan ◽  
Thi Ngoc Tran ◽  
I-Hong Wen ◽  
Van Bon Nguyen ◽  
Anh Dzung Nguyen ◽  
...  
Keyword(s):  
Alkaline Protease ◽  
Nitrogen Nutrition ◽  
Crab Shell ◽  
Carbon And Nitrogen ◽  
Optimal Activity ◽  
Shrimp Shell ◽  
Paenibacillus Sp ◽  
Shrimp Head ◽  
Ph Range ◽  
By Products

Fishery processing by-products have been of great interest to researchers due to their beneficial applications in many fields. In this study, five types of marine by-products, including demineralized crab shell, demineralized shrimp shell, shrimp head, shrimp shell, and squid pen, provided sources of carbon and nitrogen nutrition by producing a protease from Paenibacillus sp. TKU047. Strain TKU047 demonstrated the highest protease productivity (2.98 U/mL) when cultured for two days on a medium containing 0.5% of shrimp head powder (SHP). The mass of TKU047 protease was determined to be 32 kDa (approximately). TKU047 protease displayed optimal activity at 70–80 °C and pH 9, with a pH range of stability from 6 to 11. TKU047 protease also showed stability in solutions containing surfactants and detergents. Based on its excellent properties, Paenibacillus sp. TKU047 protease may be a feasible candidate for inclusion in laundry detergents.

Carbon and nitrogen nutrition of Trichoderma

1973 ◽  
Vol 5 (5) ◽  
pp. 505-515 ◽  
Author(s):  
R.M. Danielson ◽  
C.B. Davey
Keyword(s):  
Nitrogen Nutrition ◽  
Carbon And Nitrogen

The Carbon and Nitrogen Nutrition of Fusarium roseum

Mycologia ◽  
1965 ◽  
Vol 57 (6) ◽  
pp. 897 ◽  
Author(s):  
M. E. Lopez ◽  
C. L. Fergus
Keyword(s):  
Nitrogen Nutrition ◽  
Carbon And Nitrogen
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