Anaerobic Co-Digestion of Cow Manure and Palm Oil Mill Effluent (POME): Assessment of Methane Production and Biodegradation Efficiency

The performance of anaerobic co-digestion of cow manure and POME was evaluated. The anaerobic composting process was carried out by using semi-continuous reactors under the mesophilic condition (35 ± 1℃). The addition of POME to the on-going anaerobic composting of cow manure was applied stepwise within a cycle of HRT (20 days). Results showed that the anaerobic co-digestion reactor could produce methane at about six times higher (7.2 L CH4) than the control reactor (1.3 L CH4). An increasing of POME loaded to the on-going anaerobic composting cow manure culture (4% to 64%) did not affect pH of the culture in which pH was still stable between 7.11 and 7.5. Assessment of biodegradation efficiency revealed that nitrogen removal of the anaerobic co-digestion reactor was six times higher (21%) than the nitrogen removal of the control reactor (3.4%). This suggested that the anaerobic co-digestion reactor performed sufficiently well in which no organic acid as well as ammonia accumulated in the reactor that could be effective to decompose the organic matters.

Effects of Adding Laccase to Bacterial Consortia Degrading Heavy Oil

High-efficiency bioremediation technology for heavy oil pollution has been a popular research topic in recent years. Laccase is very promising for the remediation of heavy oil pollution because it can not only convert bio-refractory hydrocarbons into less toxic or completely harmless compounds, but also accelerate the biodegradation efficiency of heavy oil. However, there are few reports on the use of laccase to enhance the biodegradation of heavy oil. In this study, we investigated the effect of laccase on the bacterial consortia degradation of heavy oil. The degradation efficiencies of bacterial consortia and the laccase-bacterial consortia were 60.6 ± 0.1% and 68.2 ± 0.6%, respectively, and the corresponding heavy oil degradation rate constants were 0.112 day−1 and 0.198 day−1, respectively. The addition of laccase increased the heavy oil biodegradation efficiency (p < 0.05) and biodegradation rate of the bacterial consortia. Moreover, gas chromatography–mass spectrometry analysis showed that the biodegradation efficiencies of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 82.5 ± 0.7% and 76.2 ± 0.9%, respectively, which were 16.0 ± 0.3% and 13.0 ± 1.8% higher than those of the bacterial consortia, respectively. In addition, the degradation rate constants of the laccase-bacterial consortia for saturated hydrocarbons and aromatic hydrocarbons were 0.267 day−1 and 0.226 day−1, respectively, which were 1.07 and 1.15 times higher than those of the bacterial consortia, respectively. The degradation of C15 to C35 n-alkanes and 2 to 5-ring polycyclic aromatic hydrocarbons by laccase-bacterial consortia was higher than individual bacterial consortia. It is further seen that the addition of laccase significantly improved the biodegradation of long-chain n-alkanes of C22–C35 (p < 0.05). Overall, this study shows that the combination of laccase and bacterial consortia is an effective remediation technology for heavy oil pollution. Adding laccase can significantly improve the heavy oil biodegradation efficiency and biodegradation rate of the bacterial consortia.