Isolation and characterization of plasmid from the Bacillus brevis var. G.-B. cells

1978 ◽  
Vol 164 (2) ◽  
pp. 195-204 ◽  
Author(s):  
A. P. Dobritsa ◽  
Svetlana V. Dobritsa ◽  
V. I. Tanyashin
Keyword(s):  
B Cells ◽  
Bacillus Brevis ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Single Anti-U1A-specific B Cells from Autoimmune Patients

1997 ◽  
Vol 815 (1 B-Lymphocytes) ◽  
pp. 440-442 ◽  
Author(s):  
RUUD M. T. DE WILDT ◽  
FRANK H. J. VAN DEN HOOGEN ◽  
WALTHER J. VENROOIJ ◽  
RENÉ M. A. HOET
Keyword(s):  
B Cells ◽  
Isolation And Characterization

Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48)

1991 ◽  
Vol 11 (3) ◽  
pp. 1614-1623
Author(s):  
R C Fisher ◽  
D A Thorley-Lawson
Keyword(s):  
B Cells ◽  
Epstein Barr Virus ◽  
Leukocyte Adhesion ◽  
Human Leukocyte ◽  
Lymphoid Cells ◽  
Immunoglobulin Superfamily ◽  
Barr Virus ◽  
Isolation And Characterization ◽  
Epstein Barr

BLAST-1 (CD48) (previously referred to as BCM-1 by the Human Gene Nomenclature Committee) is an early-activation-associated membrane glycoprotein expressed on the surface of human leukocytes and induced to a high level following infection of B cells by the Epstein-Barr virus. It is a member of the immunoglobulin superfamily, mediates cell adhesion, and has significant sequence homology to two other adhesion molecules, CD2 and LFA3. Here we report the isolation and characterization of the BLAST-1 gene. The gene is at least 28.6 kb in length, is split into 4 exons, and contains a restriction fragment-length polymorphism. The overall genomic organization is consistent with other members of the immunoglobulin superfamily, in which extracellular immunoglobulinlike domains are encoded by discrete exons. Transcription is initiated at a series of major and minor sites in both normal and tumor-derived lymphoid cells. Appropriately located TATA and CCAAT box sequences were not detected. These characteristics have also been demonstrated for the recently described B-cell-specific genes B29 and CD20. The expression of these genes in B cells may involve the use of multiple promoters and novel transcription initiator-binding proteins. A 1.58-kb genomic DNA fragment, consisting of the 5'-flanking region located immediately upstream of the ATG initiation codon, was able to drive the expression of a reporter gene in an orientation-dependent and tissue-restricted manner.

scholarly journals Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48).

1991 ◽  
Vol 11 (3) ◽  
pp. 1614-1623 ◽  
Author(s):  
R C Fisher ◽  
D A Thorley-Lawson
Keyword(s):  
B Cells ◽  
Epstein Barr Virus ◽  
Leukocyte Adhesion ◽  
Human Leukocyte ◽  
Lymphoid Cells ◽  
Immunoglobulin Superfamily ◽  
Barr Virus ◽  
Isolation And Characterization ◽  
Epstein Barr

BLAST-1 (CD48) (previously referred to as BCM-1 by the Human Gene Nomenclature Committee) is an early-activation-associated membrane glycoprotein expressed on the surface of human leukocytes and induced to a high level following infection of B cells by the Epstein-Barr virus. It is a member of the immunoglobulin superfamily, mediates cell adhesion, and has significant sequence homology to two other adhesion molecules, CD2 and LFA3. Here we report the isolation and characterization of the BLAST-1 gene. The gene is at least 28.6 kb in length, is split into 4 exons, and contains a restriction fragment-length polymorphism. The overall genomic organization is consistent with other members of the immunoglobulin superfamily, in which extracellular immunoglobulinlike domains are encoded by discrete exons. Transcription is initiated at a series of major and minor sites in both normal and tumor-derived lymphoid cells. Appropriately located TATA and CCAAT box sequences were not detected. These characteristics have also been demonstrated for the recently described B-cell-specific genes B29 and CD20. The expression of these genes in B cells may involve the use of multiple promoters and novel transcription initiator-binding proteins. A 1.58-kb genomic DNA fragment, consisting of the 5'-flanking region located immediately upstream of the ATG initiation codon, was able to drive the expression of a reporter gene in an orientation-dependent and tissue-restricted manner.

ISOLATION AND CHARACTERIZATION OF DIFFERENT TYPES OF PANCREATIC ISLET CELLS IN GUINEA-PIGS

1966 ◽  
Vol 53 (3) ◽  
pp. 480-488 ◽  
Author(s):  
Birger Petersson
Keyword(s):  
B Cells ◽  
Islet Cells ◽  
Dry Mass ◽  
Endocrine Activity ◽  
Isolation And Characterization ◽  
Different Types ◽  
Gentle Pressure ◽  
Staining Methods ◽  
Isolated Islet Cells

ABSTRACT By means of gentle pressure on isolated guinea-pig islets, cells in an isolated state were obtained. The free islet cells could be classified as A1, A2 and B cells with the current staining methods. Determinations of the dry mass with a scanning interference microphotometer showed a significantly higher value in the A2 cells (1.95 × 10−10 g) as compared with the B cells (1.12 × 10−10 g). Various possibilities for utilizing the isolated islet cells for further analyses of the endocrine activity of the pancreas are discussed.

Isolation and characterization of polyadenylate-containing RNA from Bacillus brevis

Biochemistry ◽  
1978 ◽  
Vol 17 (17) ◽  
pp. 3468-3474 ◽  
Author(s):  
Nilima Sarkar ◽  
Donna Langley ◽  
Henry Paulus
Keyword(s):  
Bacillus Brevis ◽  
Isolation And Characterization

Isolation and characterization of human B lymphocyte enriched populations. I. Purification of B cells by immune rosette depletion

1983 ◽  
Vol 61 (3) ◽  
pp. 283-292 ◽  
Author(s):  
Kenneth C. Anderson ◽  
James D. Griffin ◽  
Michael P. Bates ◽  
Bruce L. Slaughenhoupt ◽  
Stuart F. Schlossman ◽  
...  
Keyword(s):  
B Cells ◽  
B Lymphocyte ◽  
Isolation And Characterization

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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