Effects of hemolysis and storage condition on neuron-specific enolase (NSE) in cerebrospinal fluid and serum: implications in clinical practice

2005 ◽  
Vol 43 (11) ◽  
Author(s):  
Laurent Ramont ◽  
Henri Thoannes ◽  
Ariel Volondat ◽  
François Chastang ◽  
Marie-Christine Millet ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Clinical Practice ◽  
Neurodegenerative Diseases ◽  
Neuron Specific Enolase ◽  
Storage Condition ◽  
Storage Conditions ◽  
Serum Samples ◽  
Duration Of Storage ◽  
And Storage

AbstractThe concentration of neuron-specific enolase (NSE) in serum and cerebrospinal fluid (CSF) has been used as a biomarker in some cancers and, more recently, in neurodegenerative diseases. Pre-analytical conditions are very important for the quality of returned results. In this study, we evaluated the effects of storage conditions (temperature and duration of storage) and hemolysis on the concentration of NSE in serum and CSF. Our results demonstrate that samples for NSE measurement may be stored at −80°C for no more than 6 months in the case of CSF and 9months in the case of serum samples. Even invisible hemolysis may increase NSE levels in samples. Consequently, an index of hemolysis should be determined before deciding whether or not to perform NSE measurement.

scholarly journals The Optimum Storage Conditions on the Quality of the Stingless bee Honey

2021 ◽  
Vol 2049 (1) ◽  
pp. 012003
Author(s):  
Imron Meechai ◽  
Isma-ae Chelong ◽  
Romlee Chedoloh
Keyword(s):  
Moisture Content ◽  
Physicochemical Properties ◽  
Sugar Content ◽  
Storage Condition ◽  
Storage Conditions ◽  
Stingless Bee ◽  
Significant Difference ◽  
Storage Times ◽  
And Storage

Abstract Honey of stingless bee has a higher moisture content than bees. Long-term storage may cause fermentation processes to change the physicochemical properties and taste. Thus, the aim of this research was evaluation of the optimum storage condition on the quality of stingless bee honey. Stingless bee honey (Tetragonular larviceps) was contained in plastic bottle and kept at ambient temperature (30-35°C) and low temperature (4-8°C) for 0-45 days. Before and after storage honey were analyzed the physicochemical properties and sensory investigation for comparison of quality. The results showed that temperatures and storage times have affected on the reducing sugar content, pH, conductivity, color, moisture content with significant difference (p<0.05). While, temperatures and storage times have unaffected on the °Brix value (p>0.05). Additionally, the physicochemical properties of honey were according with previously quality report. The sensory investigation indicated that the smell natural flavor, consistency, taste and sourness were not significant difference (p>0.05). In contrast, the color and overall preference were significant difference (p<0.05). For honey quality, Thus, the honey might keep at 4-35°C for ≤45 day of this study.

scholarly journals Impact of Chemicals and Storage Conditions on Post-harvest Quality of Litchi (Litchi chinensis Sonn.) cv. China

2021 ◽  
pp. 17-25
Author(s):  
Mary Sumi ◽  
Animesh Sarkar ◽  
C. S. Maiti ◽  
Akali Sema ◽  
S. P. Kanaujia
Keyword(s):  
Room Temperature ◽  
Calcium Nitrate ◽  
Storage Condition ◽  
Storage Conditions ◽  
Hot Water ◽  
Soluble Solids ◽  
Anthocyanin Content ◽  
Litchi Fruit ◽  
And Storage

The efficacy of chemical dips and different storage condition, alone and in combination were imposed in freshly harvested litchi fruit to investigate the changes of bio-chemical composition of fruits at regular interval for a certain period of storage. The pre-cooled litchi fruits after disinfectant were dipped for 60 seconds in lecithin (0.5% & 1%), Hot water, HCl (3%) with hot water, CaCl2 (1% & 2%), CaSO4 (1% & 2%), wax emulsion (5% & 10%), CaNO3 (0.5% & 1%), Chitosan (1% & 2%) and Kaolin (1% & 2%). The treated fruits were stored at room temperature as well as at 4°C & 90% R.H and the quality attributes were assessed at 2, 4, 6 and 8 days interval. Calcium nitrate @ 1% under refrigerated conditions recorded maximum ascorbic acid (54.50 mg/100g pulp), fruit firmness (1.75 kg cm-2) and the lowest acidity (0.42%). Total soluble solids (18.17°B), total sugar (11.27%) and polymeric colour retention with high anthocyanin content (48.94 mg/100g pulp) in peel was found in treated fruits with calcium chloride @1% at 4°C & 90% R.H even after 8 days of storage.

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

scholarly journals Effects of packaging and storage conditions on the quality of amoxicillin-clavulanic acid – an analysis of Cambodian samples

2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Mohiuddin Hussain Khan ◽  
Kirara Hatanaka ◽  
Tey Sovannarith ◽  
Nam Nivanna ◽  
Lidia Cecilia Cadena Casas ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Storage Conditions ◽  
Amoxicillin Clavulanic Acid ◽  
And Storage

scholarly journals A review on potato microtuber storability and dormancy

2016 ◽  
Vol 8 (4) ◽  
pp. 2319-2324 ◽  
Author(s):  
Murlidhar J. Sadawarti ◽  
K. K. Pandey ◽  
B. P. Singh ◽  
R. K. Samadiya
Keyword(s):  
Significant Role ◽  
Carbon Disulphide ◽  
Potato Production ◽  
Storage Condition ◽  
Storage Conditions ◽  
Dormancy Breaking ◽  
Tuber Dormancy ◽  
Seed Potato Production ◽  
Sprouting Ability ◽  
And Storage

Potato microtubers plays important role in seed potato production technology as it has great advantage of storage, transport and mechanization due to their little size and reduced weight. Dormancy in potato microtubers is very important and storage conditions as well as size of microtubers influences the dormancy in microtubers. Increasing size of the micro-tuber resulted in significant increase in the viability and sprouting ability of microtubers with reduced durations of dormancy and weight loss at the end of storage. Small microtubers are more vulnerable to storage damage. The larger microtubers lost moisture content more slowly and retained firmness longer when stored at 40C. Development of dormancy during storage strongly affected by the storage condition especially the temperature regime, the presence of light and the relative humidity. The dormancy duration was linearly and inversely correlated with the length of storage. Storage containers and conditions are also important for microtuber storage. Endogenous hormones ABA, ethylene, cyokinin and gibberllic acid play a significant role in tuber dormancy regulation.Microtubers with thick diameter which have passed more times in dormancy and have better functionality than small microtubers with less time in dormancy. Growth regulators like gibberellic acid, thiourea, gibberllic acid + thiourea, randite and carbon disulphide plays significant role in dormancy breaking of microtubers.

scholarly journals Nanocomposite coatings for hatching eggs and table eggs

2021 ◽  
Vol 6 (1) ◽  
pp. 573-586
Author(s):  
Oleksandr Chekh ◽  
Olga Bordunova ◽  
Vadym Chivanov ◽  
Evgenia Yadgorova ◽  
Larisa Bondarchuk
Keyword(s):  
Pathogenic Bacteria ◽  
Protective Coatings ◽  
Storage Conditions ◽  
Hatching Rate ◽  
Green Technologies ◽  
Egg Hatching ◽  
Poultry Products ◽  
Hatching Eggs ◽  
And Storage

Abstract Theoretical and applied aspects of the concept of using biomimetic protective coatings GREEN ARTICLE (ARTIficial cutiCLE) in the poultry industry, namely in the production of table and hatching eggs, are developed. The basic matrix component of the protective coatings GREEN ARTICLE is chitosan, an environmentally friendly, inexpensive, and harmless material. It is experimentally proved that electrochemical and ultrasonic technologies for modifying a solution of chitosan in peroxide compounds (peracetic acid and hydrogen peroxide) with nanoparticles of oxides like titanium, iron, zinc, and metals, such as titanium, copper, and calcite, allow to create protective coatings of double action in accordance with the technologies: (a) extending the shelf life of table eggs, food green article (FGA) and (b) preventing contamination of hatching eggs with pathogenic microflora, increasing the hatchability of eggs, and the quality of chicken, hatching green article (HGA). In the technology of storing table eggs, artificial cuticles based on chitosan FGA are characterized by the following characteristics: increased thickness of 6–10 µm, low gas and moisture permeability, and high biocidal activity against pathogenic bacteria and viruses. It is shown that the use of FGA technology in the production of table eggs can extend the period of transportation and storage of products by 33–35 days at a temperature of 24°C and maintained a grade A (Haugh unit = 71–60) through the entire 35 days period. The technology HGA reduces the rate of contamination of hatching eggs with pathogenic microflora by 99.29–99.7%, while increasing the egg hatching rate by 2.3–11.6% compared to the control, depending on the cross of the poultry and the storage conditions of the hatching eggs. It is important that these technologies have a great prospect for application in countries that develop green technologies for the production of poultry products.

scholarly journals An Investigation of Moroccan Vinegars: Their Physicochemical Properties and Antioxidant and Antibacterial Activities

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Driss Ousaaid ◽  
Hamada Imtara ◽  
Hassan Laaroussi ◽  
Badiaa Lyoussi ◽  
Ilham Elarabi
Keyword(s):  
Physicochemical Properties ◽  
Bioactive Compounds ◽  
Antibacterial Activities ◽  
Storage Conditions ◽  
Production Methods ◽  
Maximum Value ◽  
Processing And Storage ◽  
And Storage ◽  
Excellent Source

Vinegar is a natural product rich in bioactive compounds such as phenols, flavonoids, and organic acids. Several factors affect the quality of vinegars such as apple origin, environmental conditions, production methods, processing, and storage conditions. We investigated the quality of apple vinegars as well as their physicochemical properties and the antioxidant and antibacterial activities of vinegars collected from different areas in Morocco. For physicochemical properties, the outcomes showed the following values: pH (3.18–3.83), electrical conductivity (2.11–2.90), acidity (0.24–5.6), Brix (3.25–6), and density (1.0123–1.0233). The polyphenols content of samples was 68.91 mg AG/100 mL in sample S6 as the minimum value and 147.54 mg AG/100 mL in sample S2 as the maximum value. The best ability to scavenge the DPPH radical was observed in sample S3 (IC50 = 0.45 ± 0.013 µL/mL). Sample S2 showed moderate antibacterial effect against microorganisms tested with MICs ranging from 0.78 µL/mL to 1.125 µL/mL and with a diameter of inhibition ranging from 15.65 mm to 27.65 mm. In addition, a strong correlation was observed between the antibacterial activity of vinegars and physicochemical parameters (pH and total acidity). These outcomes have shown that our vinegar samples are an excellent source of bioactive compounds with potent antioxidant and antibacterial potentials.

Effect of centre of production, maturity and storage treatment of seed tubers on the growth of early potatoes: I. Sprout development in storage

1969 ◽  
Vol 73 (2) ◽  
pp. 161-166 ◽  
Author(s):  
P. B. Goodwin ◽  
A. Brown ◽  
J. H. Lennard ◽  
F. L. Milthorpe
Keyword(s):  
Storage Conditions ◽  
Southern Region ◽  
Post Harvest ◽  
Long Period ◽  
Temperature Conditions ◽  
Seed Tubers ◽  
Storage Treatment ◽  
Sprout Length ◽  
Duration Of Storage ◽  
And Storage

SUMMARYThe effect of three centres of production, two times of lifting and two post-harvest treatments of apparently virus-free seed tubers on sprout development at planting was studied over two seasons. Sprout length and degree of development were determined mainly by the light and temperature conditions during storage. Under similar storage conditions tubers from the most southern region, lifted early and allowed to sprout from lifting produced longer sprouts than those from the more northerly regions, lifted late and sprouted from midwinter. At planting all tubers had sprouts longer than 2·5 cm. Desprouted tubers from the more southern regions had the highest number of sprouts at planting and tubers 'greened' immediately on lifting had the least.An index of sprout development at planting was defined. The conditions and duration of storage had most influence on the degree of development as measured by this index; sprouts were most developed on tubers sprouted over a long period and on those sprouted in a heated glasshouse. The effects of varying centres of production and times of lifting on sprout development at planting were small compared with those readily produced by varying the storage conditions.

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