Low doses of γ-radiation induce consistent protein expression changes in human leukocytes

2011 ◽  
Vol 8 (5/6) ◽  
pp. 374 ◽  
Author(s):  
Sara Skiöld ◽  
Susanne Becker ◽  
Ulf Hellman ◽  
Gert Auer ◽  
Ingemar Näslund ◽  
...  
Keyword(s):  
Protein Expression ◽  
Low Doses ◽  
Γ Radiation ◽  
Human Leukocytes

Low doses of α- and γ-radiation enhance DNA thermal stability

1999 ◽  
Vol 80 (2) ◽  
pp. 103-118 ◽  
Author(s):  
A.G Georgakilas ◽  
E.G Sideris ◽  
L Sakelliou ◽  
C.A Kalfas
Keyword(s):  
Thermal Stability ◽  
Low Doses ◽  
Γ Radiation

scholarly journals Effects of low doses of gamma rays on the stability of normal and diabetic erythrocytes.

2011 ◽  
Vol 58 (4) ◽  
Author(s):  
Magdalena Kaczmarska ◽  
Zofia Kopyściańska ◽  
Maria Fornal ◽  
Tomasz Grodzicki ◽  
Krzysztof Matlak ◽  
...  
Keyword(s):  
Diabetic Patients ◽  
Low Doses ◽  
Γ Radiation ◽  
Human Red Blood Cells ◽  
Healthy Donors ◽  
Protein Membrane ◽  
Applied Dose ◽  
The Stability ◽  
Membrane Components ◽  
Enzymatic Pathways

We studied the influence of low doses of γ radiation (from 0.04 to 1.8 mGy) on the stability of human red blood cells (RBC) from healthy donors and diabetic patients using absorption spectroscopy. Because of the alteration of many enzymatic pathways in diabetic RBCs resulting in strong modification of the lipid and protein membrane components one could expect that the ionizing γ-radiation should influence the stability of the healthy and diabetic cells in a different way. Indeed, distinct discontinuities and monotonic changes of hemolysis detected in the healthy and diabetic RBCs suggest that various enzymatic and chemical processes are activated in these membranes by γ radiation. Mössbauer measurements showed that only the highest applied dose of γ radiation caused modification of hemoglobin in both types of RBCs.

The response of mouse embryonic stem cells to low doses of γ-radiation: Evidence for an adaptive response

2014 ◽  
Vol 761 ◽  
pp. 44-47 ◽  
Author(s):  
Hamid Kalantari ◽  
Nasrin Motamed ◽  
Anahita Mohseni Meybodi ◽  
Ali Jabbari Arfaie ◽  
Hossein Baharvand ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Adaptive Response ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Low Doses ◽  
Γ Radiation

Explanation of protective effects of low doses of γ -radiation with a mechanistic radiobiological model

2002 ◽  
Vol 78 (12) ◽  
pp. 1159-1173 ◽  
Author(s):  
H. Schöllnberger ◽  
R. E. J. Mitchel ◽  
E. I. Azzam ◽  
D. J. Crawford-Brown ◽  
W. Hofmann
Keyword(s):  
Low Doses ◽  
Protective Effects ◽  
Γ Radiation ◽  
Radiobiological Model

scholarly journals Erectile Dysfunction Drugs Changed the Protein Expressions and Activities of Drug-Metabolising Enzymes in the Liver of Male Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Salah A. Sheweita ◽  
Mona Wally ◽  
Mostafa Hassan
Keyword(s):  
Erectile Dysfunction ◽  
Protein Expression ◽  
Phase I ◽  
The Other ◽  
Male Rats ◽  
Low Doses ◽  
Other Hand ◽  
Endogenous Compounds ◽  
High Doses ◽  
Drug Metabolising Enzymes

Erectile dysfunction (ED) is a major health problem and is mainly associated with the persistent inability of men to maintain sufficient erection for satisfactory sexual performance. Millions of men are using sildenafil, vardenafil, and/or tadalafil for ED treatment. Cytochrome P450s (CYPs) play a central role in the metabolism of a wide range of xenobiotics as well as endogenous compounds. Susceptibility of individuals to the adverse effects of different drugs is mainly dependent on the expression of CYPs proteins. Therefore, changes in activities of phase I drug-metabolising enzymes [arylhydrocarbon hydroxylase (AHH), dimethylnitrosamine N-demethylase (DMN-dI), 7-ethoxycoumarin-O-deethylase (ECOD), and ethoxyresorufin-O-deethylase ((EROD)] and the protein expression of different CYPs isozymes (CYP1A2, CYP2E1, CYP2B1/2, CYP3A4, CYP2C23, and CYP2C6) were determined after treatment of male rats with either low or high doses of sildenafil (Viagra), tadalafil (Cialis), and/or vardenafil (Levitra) for 3 weeks. The present study showed that low doses of tadalafil and vardenafil increased DMN-dI activity by 32 and 23%, respectively. On the other hand, high doses of tadalafil, vardenafil, and sildenafil decreased such activity by 50, 56, and 52%, respectively. In addition, low doses of tadalafil and vardenafil induced the protein expression of CYP2E1. On the other hand, high doses of either tadalafil or sildenafil were more potent inhibitors to CYP2E1 expression than vardenafil. Moreover, low doses of both vardenafil and sildenafil markedly increased AHH activity by 162 and 247%, respectively, whereas high doses of tadalafil, vardenafil, and sildenafil inhibited such activity by 36, 49, and 57% and inhibited the EROD activity by 39, 49, and 33%, respectively. Low and high doses of tadalafil, vardenafil, and sildenafil inhibited the activity of NADPH-cytochrome c reductase as well as its protein expression. In addition, such drugs inhibited the expression of CYP B1/2 along with its corresponding enzyme marker ECOD activity. It is concluded that changes in the expression and activity of phase I drug-metabolising enzymes could change the normal metabolic pathways and might enhance the deleterious effects of exogenous as well as endogenous compounds.

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