scholarly journals Recurrent ADCY5 Mutation in Mosaic Form with Nocturnal Paroxysmal Dyskinesias and Video Electroencephalography Documentation of Dramatic Response to Caffeine Treatment

2020 ◽  
Vol 13 (3) ◽  
pp. 238-240 ◽  
Author(s):  
Kuldeep Shetty ◽  
Asodu Sandeep Sarma ◽  
Meera Devan ◽  
Ashwin Dalal ◽  
Gopal Krishna Dash ◽  
...  
Keyword(s):  
Caffeine Treatment ◽  
Mosaic Form

46,XX/47,XXY mosaic form of klinfelter syndrome with ambiguous genitalia: a case report

2014 ◽  
Author(s):  
Fadila Chabour ◽  
Said Azzoug ◽  
Leila Rabehi ◽  
Farida Chentli
Keyword(s):  
Case Report ◽  
Ambiguous Genitalia ◽  
Mosaic Form

scholarly journals Attenuation of endoplasmic reticulum stress by caffeine ameliorates hyperoxia-induced lung injury

2017 ◽  
Vol 312 (5) ◽  
pp. L586-L598 ◽  
Author(s):  
Ru-Jeng Teng ◽  
Xigang Jing ◽  
Teresa Michalkiewicz ◽  
Adeleye J. Afolayan ◽  
Tzong-Jin Wu ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Lung Injury ◽  
Er Stress ◽  
Saline Control ◽  
Sprague Dawley ◽  
Chemical Chaperone ◽  
Stress Markers ◽  
Rat Pups ◽  
Caffeine Treatment ◽  
Downstream Effectors

Rodent pups exposed to hyperoxia develop lung changes similar to bronchopulmonary dysplasia (BPD) in extremely premature infants. Oxidative stress from hyperoxia can injure developing lungs through endoplasmic reticulum (ER) stress. Early caffeine treatment decreases the rate of BPD, but the mechanisms remain unclear. We hypothesized that caffeine attenuates hyperoxia-induced lung injury through its chemical chaperone property. Sprague-Dawley rat pups were raised either in 90 (hyperoxia) or 21% (normoxia) oxygen from postnatal day 1 (P1) to postnatal day 10 (P10) and then recovered in 21% oxygen until P21. Caffeine (20 mg/kg) or normal saline (control) was administered intraperitoneally daily starting from P2. Lungs were inflation-fixed for histology or snap-frozen for immunoblots. Blood caffeine levels were measured in treated pups at euthanasia and were found to be 18.4 ± 4.9 μg/ml. Hyperoxia impaired alveolar formation and increased ER stress markers and downstream effectors; caffeine treatment attenuated these changes at P10. Caffeine also attenuated the hyperoxia-induced activation of cyclooxygenase-2 and markers of apoptosis. In conclusion, hyperoxia-induced alveolar growth impairment is mediated, in part, by ER stress. Early caffeine treatment protects developing lungs from hyperoxia-induced injury by attenuating ER stress.

Effects of caffeine treatment on aged porcine oocytes: parthenogenetic activation ability, chromosome condensation and development to the blastocyst stage after somatic cell nuclear transfer

Zygote ◽  
2005 ◽  
Vol 13 (4) ◽  
pp. 335-345 ◽  
Author(s):  
Masaki Iwamoto ◽  
Akira Onishi ◽  
Dai-ichiro Fuchimoto ◽  
Tamas Somfai ◽  
Shun-ichi Suzuki ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Blastocyst Stage ◽  
The Other ◽  
Control Group ◽  
Blastocyst Formation ◽  
Cell Nuclei ◽  
Somatic Nucleus ◽  
Membrane Breakdown ◽  
Caffeine Treatment ◽  
M Phase

The possibility of using aged porcine oocytes treated with caffeine, which inhibits the decrease in M-phase promoting factor activity, for pig cloning was evaluated. Cumulus–oocyte complexes (COCs) were cultured initially for 36h and subsequently with or without 5mM caffeine for 24h (in total for 60h: 60CA+ or 60CA– group, respectively). As a control group, COCs were cultured for 48h without caffeine (48CA–). The pronuclear formation rates at 10h after electrical stimulation in the 60CA+ and 60CA– groups decreased significantly (p<0.05) compared with the 48CA– group. However, the fragmentation rate was significantly higher (p<0.05) in the 60CA– group than in the 60CA+ and 48CA– groups. When the stimulated oocytes were cultured for 6 days, the 60CA+ group showed significantly lower blastocyst formation and higher fragmentation or degeneration rates (p<0.05) than the 48CA– group. However, the number of total cells in blastocysts was not affected by maturation period or caffeine treatment. When somatic cell nuclei were injected into the non-enucleated oocytes and exposed to cytoplasm for a certain duration (1–11h) before the completion of maturation (48 or 60h), the rate of nuclear membrane breakdown after exposure to cytoplasm for 1–2h in the 60CA– oocytes was significantly lower (p;<0.05) than in the other experimental groups. The rate of scattered chromosome formation in the same 60CA– group tended to be lower (p=0.08) than in the other groups. After the enucleation and transfer of nuclei, blastocyst formation rates in the 60CA+ and 60CA– groups were significantly lower (p<0.05) than in the 48CA– group. Blastocyst quality did not differ among all the groups. These results suggest that chromosome decondensation of the transplanted somatic nucleus is affected by both the duration of exposure to cytoplasm and the age of the recipient porcine oocytes, and that caffeine treatment promotes nuclear remodelling but does not prevent the decrease in the developmental ability of cloned embryos caused by oocyte aging.

The Effect of Long Term Caffeine Treatment on Hypoxic-Ischemic Brain Damage in the Neonate

1995 ◽  
Vol 38 (3) ◽  
pp. 312-318 ◽  
Author(s):  
Elsa Bona ◽  
Ulrika Ådén ◽  
Bertil B Fredholm ◽  
Henrik Hagberg
Keyword(s):  
Brain Damage ◽  
Ischemic Brain Damage ◽  
Ischemic Brain ◽  
Caffeine Treatment

scholarly journals Pengaruh Kafein Terhadap Lengkung Ekor Spermatozoa Epididimis Sapi Bali

2019 ◽  
Vol 2 (2) ◽  
pp. 143
Author(s):  
Oktora Dwi Putranti
Keyword(s):  
Sperm Motility ◽  
Slaughter House ◽  
Caffeine Treatment ◽  
Tail Movement

This study aims to analyze the influence of caffeine on the distance of the tail movement of the spermatozoa epididymis cattle Bali. Methods in this study with a collection of sperm from Slaughter House (RPH) and then conducted the process of freezing with Tris yolk dilution. Treatment of caffeine with levels of 0 mg/ml, 2 mg/ml, 4 mg/ml and 6 mg/ml in spermatozoa that have been in thawing. The variables observed were DCL, DAP, DSL, ALH analyzed by CASA. Results showed no difference (P> 0.05) motility with caffeine treatment and short sperm tailwave distance of 37.27 μm showed the sperm motility velocity. Keywords: spermatozoa tail, caffeine

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